LOCUS       sequence-306814-       13532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Nup124.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 13532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-P41nmt1-Nup124-GFP-MTn
FEATURES             Location/Qualifiers
     source          1..13532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2148..2159)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5153..5866
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6082..6269
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6137..6161)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(7933..8727)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      9336..10119
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(10842..10859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10842..10858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10851..10873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11071..11526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11158..11177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11368..11389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11543..11562)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11662..11684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11722..11740)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11808..11912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11913..11981,11982..12773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12131..12150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12944..13532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13433..13452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagataa aggaagagga atcctggcat atcatcaatt gaataagttg
     1561 aattaattat ttcaatctca ttctcacttt ctgacttata gtcgctttgt taaagctagc
     1621 ggatccatgc ctcctgtttc aaaaaatact cgcacttcat caaagactgt taagaaaccc
     1681 tatgacccac ctcaggggag ctcaaggccc ttttttacgg tcttgaaacg ggcattttca
     1741 tctgttttgc acccattcac cagtggtcta gacgagaaag cttcaggaac ggctagcaag
     1801 gatagaaaga gtggaagagc aggtacaaaa agcctgctta ctccagagtt aacacctcat
     1861 tatttaggaa aatctccgta agttgacatt tttgtcgtga aacatattaa cattaaatag
     1921 aagaattatt cgcgttagta atcggagcca tgttagaaca attgatggaa tagaggaaaa
     1981 agttcatacg aatacatttg aacctaggaa gccaaagcaa aagcaagatt atactaattc
     2041 cccaacgctt tttaaacgcc atgacgagtt gtcgttaaaa tcacttaatt cgcttcatcc
     2101 ttcttctgct ttatccaaaa agttgggcag cacttctcaa catcaaattg caactcccaa
     2161 atcctctgct tcattgctca atatccttcg gtcacttcac gatgagcaga aaaatacctt
     2221 aaatatatca agtgtaaaac aagatcgtat taccgaagct aatcctactt gtgagaagcg
     2281 caagccttct agaagtccca gtcctatgct ttccaaaaag aaatctgttg ctcgtgcctc
     2341 tgaaaacgag ccctcagcta agcagaacaa aagcttttct ggaaatgatt cccataagag
     2401 tcttaccgac attagagata aggagaatgg cgaaacagag gtctcagcaa aaaatcacgt
     2461 tcctcaccgc tcctctcggc gacgtcgtag acaccagcgg cttatcccta tcatttatga
     2521 aactcttgaa cagatggatt taaggaagcc tgtattagtc aatgcagaag ttcagacaga
     2581 ttccaacccc ggtaatacaa tgtttattga taagcaagat atttatcacc ggttaagtac
     2641 acccacttcg cgtaagcgtc agactctcga gaaaggtcat attaaggctt tttctgcagt
     2701 tgatgaggat ttggatgaaa tctttgcttg cgaagatgac gttcattaca ccgcattacc
     2761 caaacaaaat ccaaaatctg agcgtatatt agaacctata atagcatctc ccaaggataa
     2821 tacaagtgac aagggtttat tgacgaagtc cgcccctacc tttgaagagc ttcaagcctc
     2881 tattacccct aagcctgtaa agacttctcc gaatgatacg gcacttactc tcgcgaacgc
     2941 tgaggataat aaaacctttg aacatcagcc attgtcaaag gatacagaag cgcccaaaag
     3001 ccaattttcc tcatcgccga caaaagaatc tacaactaga aagtccgaag ttgaaccgcc
     3061 atcaccttct aaggaaatca aatcttctca cttttcagta cccgaattta agtttgaacc
     3121 gaagactgaa gctaccacgg acaaaaaatt aaatgtaccc aaatttgagt ttaagccaac
     3181 tgctactgct gatgtgcaaa ctaatcgttt gaaggagaat gagcccaagc caacgttctt
     3241 tgcacaatta ccgtcaaaga ctcaagagac gccaagtata actgagaaca agccttcatt
     3301 tttttctcaa ttaagtccaa aacgagaaga aacagaaaag aaggacaacg ctccgtctgc
     3361 tcctgcttca acttctggtt tcagctttgg aggattcgca cctaaaaccc ttgaagagaa
     3421 ggaagaaacg aaagctccta cgttcaattt ctctttaaac aatgcttcta gtacacaaga
     3481 cacaacaaaa cctacgctac aatttaattt tggaagtagt tttggaaagc caacaagcaa
     3541 tattttcaat gacaaaaaaa cttctgaaaa tggtctagct agttctactg ttgcttctga
     3601 gtcaaaaccg tcagctcccg aatcaaaacc gtcctcgggt tttggaaata ccgcaggctc
     3661 atcacccttt tcattcaatt tgaccaagga gtccaaagaa gtcccaccta caaattcctt
     3721 ttcttttgct aaaaaaggga aagatgaagc gaatgacagt ttgtcagcta aagcttctac
     3781 tccattttcg tttgcaaaac ctaatactga aaatgtaaca acaactgctc ctcagttttc
     3841 atttaatttt accaagccaa atacggatgc caaaaccaat ttgctacctg aaaaaacttt
     3901 taatgaagaa gccgtaaagc aaaaggaaac tgaaaaagag gtccctccaa caggtccaaa
     3961 agcttctgaa ataaaggatt cagtatcttc taataacgct gtgccctctt caacctttaa
     4021 ttttgtatct ccctttgctg ctgtatctga aaaaacaaat gagaataaca ttcctaacga
     4081 tacaacgaag acgaatggca atgctactaa gagaactttg gaacaaacgg aggatgcaaa
     4141 gccttttgct ttctccttcg gttccactac agaacaggca aacaaaaaag cctcaacgtc
     4201 aaatgaaact acgaagcctc aattggatac atcttctaaa actgatggag taactgctaa
     4261 tgcgccgttt agttttgcat cagcttttaa tgcacctaaa ccctcgacta atactgctga
     4321 tggcaaagat tctgcttcga atcttaccac tccttcacct gctttttcat ttggaaacaa
     4381 tagtggtgtt aaagcatcca gtaacaacaa tccctctaca aattcgtcta cggcaccgtt
     4441 ttcttttggt acatcaaata aaccggcatt tagctttggg tctgcaactt caaagactac
     4501 ctctgaagga accgctccag ctgcttctgc ttctgctcct gctcccacta cgtctgcatt
     4561 ttcttttgga gcatcaaatt ctagtatgaa taaggaagag aacactccca tggctaagga
     4621 tgctggagat acggcacctg cgtctggctt taagtctggg ttttcgttcg gtgctaacaa
     4681 ttcgcctcag cctgcttcta tgtttggtac atcgacgccg gccccgtctt ccgcgtttgc
     4741 gtttggcaac cagtcaggca cgaacccagc agctcctgca gggtttggag ggattactaa
     4801 tactgccact aataaccctc ctagtacagg ttttacattc actccatcaa atgctggatc
     4861 aactgcggct cctatgtttg gcgctggaaa cactcccaac ccatccgggt ccatcaataa
     4921 tgcatcacaa gcattcgcct tcgggtcagg agaacctagt aatccagcta gtaatccacc
     4981 tagtaccgga ttttcgttcg gtgcggctac tccgtctgcc tttaatgcaa gtgcctcaca
     5041 atcaccagcg cccaatggta ttcaattcaa tttgggctct tctaactcac aaacaaatgc
     5101 gcccccgggc cgtaaaattg ctgtggcccg aagtcgaaga aaacgtggat ccatgtctaa
     5161 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     5221 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     5281 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     5341 tttcggttat ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt
     5401 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     5461 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     5521 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     5581 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
     5641 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
     5701 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     5761 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     5821 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaaagat ctatggaccc
     5881 cgcctgtgcg tgtgcagccg gcggcgcttg cgcttgcgca ggcgcttgcg cctgcgcagc
     5941 atgcgcatgt gccgcttgcg cagcaagctg ctgcgcctgc tgtcccgtgg gctgcgccgc
     6001 atgtgccgca ggctgtgtct gcgcaggcgc cgcggacgcg tgcgcgtgct gtgccagatc
     6061 tggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     6121 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     6181 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     6241 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatac
     6301 tagtaagatg attcttttat cgtcgataac gatgaggatg tagacgatta tgatgagagt
     6361 gattagcttc taaaatttgg tgttttcaaa cgctatttac agtacaatct gaaaaaattg
     6421 gatgaacatt gtaaatggta ggatacgagt tccaattaat gtatttttta agtattacac
     6481 ttagcttatt aattttatat atacattcta cctctgtggg tttaaagaca actcgtacac
     6541 atactcaaat tatttatgaa cattataatg catattcgta ctgtctatta aacatttttt
     6601 tgacattaaa aatttcgttt actaacgtag aaagcgctga taattgaggc tactatttta
     6661 atatctagaa acaaagattc attaattaat taagtaagct ccaggatact tgtatatttc
     6721 gttaaaaaag cgcaatttca acaattccta tgaacatcca tgatttcatc acaaaatcaa
     6781 acatatcagg atttaacgtg aattatttgc tttcttgtga ttttcatctg atttctggtc
     6841 atttacgtta ctgtaaacaa gctttttaaa aaaaggattt tttacgcaat attatcgata
     6901 tcccaatctg tagtataaaa agtgcaacag tgttgagttt cccgaaacca ggcatttttg
     6961 cggtagggtc tgaaactttc acgaaaccat ttcacaacaa aacttcttca acaacatgtg
     7021 tgcaaagaaa atcgtcgtct taccaggaga ccatattggc cctgaaattg ttgcttctgc
     7081 cttggaggtt ttgaaagtcg ttgagaagaa gcgacctgag ttaaaactcg agtttgaaga
     7141 acacaagatt ggaggtgcct ctattgatgc ctatggaacc cctttgactg acgagactgt
     7201 gaaggcttgt ttggaagctg acggtgttct tttgggtgcc gttggtggtc ctgaatggac
     7261 caaccccaat tgtcgtcctg agcaaggttt attgaagctt cgtaagagta tgggtgtttg
     7321 ggccaacctt cgaccttgca actttgccag caagtcttta gtcaagtaca gccctttgaa
     7381 gcctgaaatc gttgaaggtg tcgatttttg tgttgtacga gaacttactg gaggttgtta
     7441 ctttggtgag cgcactgagg acaacggatc gggttatgct atggacactt ggccttacag
     7501 tttggaagaa gtttctcgta ttgctcgttt ggcggccgcg ggcccaacac caatgtttat
     7561 aaccaagttt tatcttgttt gtctacatgg tattttacat tcatctacat acatctttca
     7621 ttggctttgt acatagttat cattacaagt ctaaaaaaat tcactctttt cttattcaat
     7681 gtcaatccaa gagaaaagat tgtggtaatg ttgtaggagc atgtttaata aattactata
     7741 gcaaattact ttttattccc aaggtgttta tctataatag ttaatatttt agtcgctaca
     7801 taaaatttta ccaaagagta cttgtatact aattctaaat gccttctgac ataaaacgcc
     7861 taggaaaaca aacgcaaaca aggcatcgac tttttcaata accaaccaaa aaaattttac
     7921 attagtcttt ttttaatgct gagaaagtct ttgctgatat gccttccaac cagcttctct
     7981 atatctcttg gcttcgacaa caggattacg accagctcca tagactccac gaccaacaat
     8041 gatgatgtcg ctaccgcagt ttacaatcac ttcttcagga gtacgatatt gctgtcccag
     8101 cccgtctcct ttaacatcca agccgatacc aggggacata gttatgtagt cgctttgaag
     8161 gttaggaaat cgacgaccag ctataaagcc aaagcaaaaa tcggtatgct tctcaaacca
     8221 ttctaaggtt ttctctgtgt aggaaccagt agccaaagag cctttggaag acatttcagc
     8281 caaaagcaag agaccacgtc ccaaaggtaa accaacttct ttgaggcctt gtataatacc
     8341 ctcgcctggc actgtatggc aatttgtgat atgagcccaa gaagcaattt tgtacacacc
     8401 agatgcatat tgtagcttga cggtatttcc aatgtctgcg aatttgcgat cctcaaagat
     8461 aagaaaacga tgctttttac ctaaggccac cagtttttct accatatcct ggtcgaaatc
     8521 ctcgacaacg tcaatatgtg tcttgataac acagacatag ggtccaattt tatctaccaa
     8581 ttctaagatt tcggatttct tcgtcaaatc gaccgcgact gacaagttgc tttgcttttc
     8641 ttccatcaaa gccaacaatt ccttggcaat gggatttttc atcccctcag ctctagctga
     8701 atagctttga aatactctag catccataac tttgctttta aacctttaat ttcgatccaa
     8761 gcaaaaaaga ggttcttggt aggacaatac ggtaagaaaa cacgacatgt gcagagatgc
     8821 cgacgaagca tagttaaact gggatggtaa aatcaattaa gaatttataa agacaaaatt
     8881 gtataagtct ctaaaacatc ttaattatac ctcacagaac tatctaaaat atattcacaa
     8941 agtgcaaaca ttatcatgaa aaagaaccat tttaatttaa agcaagggca ttaaggctta
     9001 tttacagaat ttcttacttt tgtaaagatt ataaggctga ttatcttttt caccatgcca
     9061 aaaattacac aagatagaat ggatgtttga aattaaacgt gagtatacaa acaaatacac
     9121 taggtaaatc gaaacatttt tttctccatt aagtaacaaa ttcctattta gagaaagaat
     9181 gctgagtaga ttaaataatc tatacaaact tttttaacac aaatgcatac atatagccag
     9241 tgggatttgt agctaagctt gcgaattcga gtctaactcc ttaaccactc ggacatagtg
     9301 acttatctga cactcattgt aaattaatat atatataggc attttgttta gttaaaggta
     9361 cttaagtaat tagtataaac gaaccaattt tataatcagg aagttaagtg aatggtagca
     9421 catgtcgtaa aaattgtgaa tttttattga ataatatttt aaatacaagc ctttctagac
     9481 taggtatact cataaacata tatgagcaaa aggatagagg agattacatt gcatcttcta
     9541 caaatttatt tattgccctt tactgaaaaa ttaaataatg agtactaaat gataaaaagc
     9601 gctcagtaca agaaagattg caaaaatatt gcattcttca tgaattaaag ttgcatataa
     9661 ggcatattga aagtaatagt actaaaacag cagttagcga aaattaatag aattatattc
     9721 gcaagacaat tgtgacatta aattaaaaaa ttgtagaatt tttactatcc tctttaacgc
     9781 catgagcctt tataaaaagg ttaaattagt tttaacattc tttttttgag taagagttta
     9841 caatttatca aaacctgtgt tattatattc attagtttca atttattagc atctagagaa
     9901 aaatcaattg gcagttacat tgttggaatt tatgaagaaa aagaatctac aacggagaat
     9961 aagttgctga tcgctttccc taaaattgta tattttgctg agcttatttt gacatttcgt
    10021 tgaagttttc tctaattcgc attcatttta agtaaaacaa tgagaaataa aattacaaaa
    10081 aatacaaatt aaaatacaat ttttagctat aattatagac gatgcccttg tatcccattc
    10141 tgtctcgctt gcccctactt tttatctttt atataccata atgaacgctg ccgctactaa
    10201 ccataccccg attttacatt tcggactccc aaggacgtac aaaatagaaa actatagaaa
    10261 aaaataatca gaaaatagca tgtcatctct ttgtaaaacg cgtttgcaag aagaaaggaa
    10321 acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa gaaatcacta
    10381 acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga tggaggactc
    10441 gatttaatga attggaaggt tgggcccgcg gccgcttagc tgaaacttcc aaccctcctg
    10501 ctcccgtcac attactcgac aaagctaatg ttttggcaac ttctcgtctt tggcgcaaga
    10561 ctgtagctaa gatttttaag gaggaatatc ctcaccttac cttaaaaaac cagctcattg
    10621 actccgcagc catgcttttg gtcaagagcc ctcgtacact taacggtgtt gttttgactg
    10681 acaacttgtt tggtgacatt atttcagatg aggcttctgt cattcctggt agcttgggcc
    10741 ttttgccttc tgcctccctt tccggtgtgg taggaaaatc agaagaaaag gttcattgtt
    10801 tggttgagcc cattcacggt agcgctcccg agctcgaatt cactggccgt cgttttacaa
    10861 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    10921 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10981 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    11041 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
    11101 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
    11161 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11221 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11281 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11341 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    11401 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
    11461 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
    11521 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
    11581 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    11641 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    11701 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    11761 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    11821 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11881 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11941 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    12001 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    12061 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    12121 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    12181 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    12241 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    12301 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    12361 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    12421 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    12481 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    12541 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    12601 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    12661 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    12721 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    12781 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    13021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    13081 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    13141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    13201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    13261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    13321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    13381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    13441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    13501 gatgctcgtc aggggggcgg agcctatgga aa
//
