LOCUS       sequence-306848-       13233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to 
            produce core RNAP complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Newing TP, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, 
            Tolun G, Lewis PJ
  TITLE     Molecular basis for RNA polymerase-dependent transcription complex 
            recycling by the helicase-like motor protein HelD.
  JOURNAL   Nat Commun. 2020 Dec 18;11(1):6420. doi: 10.1038/s41467-020-20157-5.
  PUBMED    33339820
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-18"; volume: "11(1):6420. doi"; pages: " 
            10.1038/s41467-020-20157-5"
COMMENT     Sequence Label: pNG1256
FEATURES             Location/Qualifiers
     source          1..13233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2665..2684
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2835..2843
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        7519..7537
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11432..11458
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(11528..11546)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11542..11589
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(11953..11958,11959..11975,11976..11981))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(12012..12030)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12098..12202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12203..12271,12272..13063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12421..12440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag taataatttt
     2821 gtttaatcgg atcataagga ggttaatcat atgatcgaga ttgaaaaacc aaaaatcgaa
     2881 acggttgaaa tcagcgacga tgccaaattt ggtaagtttg tcgtagagcc acttgagcgt
     2941 ggatatggta caactctggg taactcctta cgtcgtatcc tcttatcctc actccctggt
     3001 gccgctgtaa catcaatcca gatagatggt gtactgcacg aattctcgac aattgaaggc
     3061 gttgtggaag atgttacaac gattatctta cacattaaaa agcttgcatt gaaaatctac
     3121 tctgatgaag agaagacgct agaaattgat gtacagggtg aaggaactgt aacggcagct
     3181 gatattacac acgatagtga tgtagagatc ttaaatcctg atcttcatat cgcgactctt
     3241 ggtgagaatg cgagtttccg agttcgcctt actgctcaaa gaggacgtgg gtatacgcct
     3301 gctgacgcaa acaagagaga cgatcagcca atcggcgtga ttccgatcga ttctatctat
     3361 acgccagttt ctcgtgtatc ttatcaggta gagaacactc gtgtaggcca agttgcaaac
     3421 tatgataaac ttacacttga tgtttggact gatggaagca ctggaccgaa agaagcaatt
     3481 gcgcttggtt caaagatttt aactgaacac cttaatatat tcgttggttt aactgacgaa
     3541 gctcaacatg ctgaaatcat ggttgaaaaa gaagaagatc aaaaagagaa agttcttgaa
     3601 atgacaattg aagaattgga tctttctgtt cgttcttaca actgcttaaa gcgtgcgggt
     3661 attaacacgg ttcaagagct cgcgaacaag acggaagaag atatgatgaa agttcgaaat
     3721 ctaggacgca aatcacttga agaagtgaaa gcgaaactag aagaacttgg actcggactt
     3781 cgcaaagacg attgactaga tgtggttttc ttaattagaa accctttttt cttttgtctt
     3841 gtaaaagtat tttctttctt tggaagaaat gcttattaca catataatac gcatgatttg
     3901 aggggtgaat cagttgacag gtcaactagt tcagtatgga cgacaccgcc agcgcagaag
     3961 ctatgctcgc attagcgaag tgttagaatt accaaatctc attgaaattc aaacctcttc
     4021 ttatcagtgg tttcttgatg agggtcttag agagatgttt caagacatat caccaattga
     4081 ggatttcact ggtaacctct ctcttgagtt cattgattat agtttaggtg agcctaaata
     4141 tcctgtagag gaatcaaaag aacgtgatgt gacttactca gctccgctaa gagtgaaggt
     4201 tcgtttaatt aacaaagaaa ctggagaggt aaaagaccaa gatgtcttca tgggtgattt
     4261 ccctattatg acagatacag gtacttttat cattaacggt gcggaacgtg ttatcgtttc
     4321 ccagcttgtt cggtctccaa gtgtatattt cagtggtaaa gtagacaaaa acggtaaaaa
     4381 aggttttacc gcaactgtca ttccaaaccg tggcgcatgg ttagaatacg aaactgatgc
     4441 gaaagatgtt gtttatgtcc gcattgatcg cacacgtaag ttgccggtta cggttctttt
     4501 gcgtgctctc ggcttcggct ccgatcaaga gattcttgat ctcataggag aaaacgaata
     4561 cctgcgaaat acgcttgata aagataacac agaaaacagc gacaaagcgt tgctggaaat
     4621 ttacgagcgt ctccgtcctg gagagccgcc tacagtagaa aatgcgaaaa gcttgcttga
     4681 ttctcgtttc tttgatccga aacgatacga tcttgccaat gtaggacgct ataaaattaa
     4741 taaaaaactt catattaaga atcgcctctt caatcagaga cttgctgaaa cgcttgttga
     4801 tcctgaaaca ggagaaatcc ttgctgaaaa aggccagatt cttgatagaa gaacgcttga
     4861 taaagtactg ccatacttag aaaacggaat cggttttaga aagctgtatc cgaatggcgg
     4921 cgttgttgaa gatgaagtga ctcttcaatc aattaaaatc tttgctccga ctgatcaaga
     4981 aggagaacag gttatcaatg taatcggcaa tgcttacatc gaagaagaga ttaaaaacat
     5041 cacgcctgct gatattattt cttcaatcag ctacttcttc aacctgctgc acggagtagg
     5101 cgacacagat gatatcgatc atcttggaaa ccgccgttta cgttctgtag gcgagcttct
     5161 ccagaaccaa ttccgtatcg gtttaagccg tatggagcgt gtggttcgtg agagaatgtc
     5221 aattcaagat acgaatacaa ttacgcctca gcagctgatc aatattcgtc ctgttattgc
     5281 gtccattaaa gagttctttg gaagctcaca gctttctcaa ttcatggatc agacgaaccc
     5341 gcttgctgaa ttaacgcaca agcgtcgtct gtcagcatta ggaccgggcg gattgacacg
     5401 tgagcgtgcc ggaatggaag tgcgtgacgt tcactactcc cactatggcc gtatgtgtcc
     5461 gattgaaacg cctgagggcc cgaacatcgg tttgatcaac tcactatcat cttatgcaaa
     5521 agtaaaccgt tttggcttta ttgaaacgcc atatcgccgc gttgaccctg aaacagggaa
     5581 ggtaacgggc agaatcgatt acttaactgc tgatgaagag gataactatg ttgtcgctca
     5641 agcgaatgct cgtcttgatg acgaaggcgc ctttattgat gacagcatcg tagctcgttt
     5701 ccgcggggag aacaccgttg tttccagaaa tcgtgtagac tacatggatg tatcgcctaa
     5761 gcaggttgta tctgctgcga cagcatgtat cccgttctta gaaaacgatg actccaaccg
     5821 tgccctcatg ggagcgaaca tgcagcgtca ggctgtgcct ttgatgcagc cggaagcgcc
     5881 atttgttgga actggtatgg aatacgtatc aggaaaagac tctggtgccg ctgttatttg
     5941 taaacaccct ggtatcgttg aacgcgtaga agccaaaaac gtttgggttc gccgttatga
     6001 agaagtagac ggtcaaaaag taaaaggaaa cctggataaa tacagcctgc tgaaatttgt
     6061 ccgctctaac caaggtacgt gctacaacca gcgtccgatc gtaagtgtcg gcgatgaagt
     6121 ggtaaaagga gaaatccttg ctgacggtcc ttctatggag cttggtgaac ttgcacttgg
     6181 ccgtaacgta atggtcggct tcatgacgtg ggatggctac aactatgagg atgccatcat
     6241 catgagtgaa cgcctagtga aggatgatgt ttatacatct atccacattg aagaatacga
     6301 atcagaagca cgtgatacga aacttggacc tgaagaaatc actcgcgata ttccaaacgt
     6361 cggtgaagat gcgcttcgca atcttgatga ccgcggaatc atccgtattg gggcagaagt
     6421 aaaagacgga gatcttcttg ttggtaaagt aacgcctaaa ggcgtaactg aactgactgc
     6481 cgaagaacgc cttcttcacg ccatctttgg cgagaaagcc cgcgaggttc gtgatacttc
     6541 tcttcgtgtg ccacatggcg gcggcggaat tatccatgac gttaaagtct tcaaccgtga
     6601 agacggagac gaacttcctc caggtgttaa ccagttagta cgcgtatata tcgttcagaa
     6661 acgtaagatt tctgaagggg ataaaatggc cggtcgtcac ggtaacaaag gtgttatctc
     6721 taagattctt cctgaagagg atatgcctta ccttcctgac ggcacaccaa ttgatatcat
     6781 gcttaacccg ctgggcgtac catcacgtat gaacatcggg caggtattgg aacttcacat
     6841 gggtatggcc gctcgttacc ttggcattca cattgcatct cctgtatttg acggagcgcg
     6901 agaagaggat gtctgggaaa cacttgaaga agccggcatg tctcgtgacg ccaaaacagt
     6961 gctttacgac ggacgtactg gagagccgtt tgataaccgt gtatctgtcg gtatcatgta
     7021 catgatcaaa ctggctcaca tggttgacga taaacttcat gcacgctcta caggccctta
     7081 ctcacttgtt acgcagcagc ctcttggcgg taaagcgcaa tttggcggac agcgttttgg
     7141 tgagatggag gtttgggcac ttgaagctta cggtgcggct tacactcttc aagaaattct
     7201 gactgttaag tctgatgacg tggttggacg tgtgaaaaca tacgaagcca tcgttaaagg
     7261 cgacaatgtt cctgaaccag gtgttccgga atcattcaaa gtattaatca aagaacttca
     7321 aagcttaggt atggatgtca aaatcctttc tggtgatgaa gaagaaatag aaatgagaga
     7381 tttagaagac gaagaagatg cgaaacaagc tgacggcctg gcattatcag gtgatgaaga
     7441 gccggaagaa acagcatctg cagacgttga acgcgatgta gtaacaaaag aataatctct
     7501 acttatagcg agcactcgta atacgactca ctataggtat tgcccatggt ttagcatacc
     7561 tctagaccaa aggagcctat gatgttagat ccgtcaattg attctttaat gaataaatta
     7621 gattcaaaat atacgctggt gactgtttct gcgagacgtg cccgtgaaat gcaaatcaaa
     7681 aaagaccaaa tgattgaaca tacgatttca cacaaatatg taggcaaagc tttagaagaa
     7741 attgatgcag gcctgctttc gtttgaaaag gaagaccgcg aataggctga catcggttaa
     7801 tccgaagata aaaagggagg taggcccctt gctagatgtg aacaattttg agtatatgaa
     7861 catcggtctt gcttcaccag ataaaatccg ttcatggtcc tttggtgaag tgaaaaagcc
     7921 tgaaacgata aactatcgta cgttaaaacc tgaaaaggac ggtctattct gcgaacgcat
     7981 tttcggaccg actaaggact gggaatgtca ttgcgggaag tacaagcgag ttcgttataa
     8041 aggcgtagtt tgtgaccgct gcggagtcga agtaacacgg gctaaagtcc gtcgtgagag
     8101 aatggggcac attgaactgg ctgccccagt ttcccacatt tggtatttca aaggtattcc
     8161 aagccgtatg ggtcttgtgc tggatatgtc acctcgtgct ttagaagaag tcatttactt
     8221 tgcttcttac gttgtaactg atccggcgaa tacaccgctt gaaaagaaac agcttctgtc
     8281 tgagaaagaa taccgtgctt atcttgataa atacggtaat aaattccaag catctatggg
     8341 tgctgaagca attcataaac ttcttcaaga tatcgatctt gtaaaagaag ttgatatgtt
     8401 aaaagaagag ctgaaaactt cacaaggaca acgccgtact cgtgcgatca aacgtcttga
     8461 agttttagaa gccttccgta actcaggaaa caagccttct tggatgatcc ttgatgtgct
     8521 tcctgttatt cctccggagc tcagaccgat ggttcagcta gatggcggac gttttgcgac
     8581 ttctgatttg aatgaccttt atcgtcgtgt catcaaccgt aacaatcgtt tgaaacgcct
     8641 tttggacctt ggtgcgccta gcatcatcgt tcaaaacgaa aagcgtatgc ttcaagaggc
     8701 tgtcgatgcc ctaattgaca acggccgccg cggacgccct gtaacaggtc ctggaaacag
     8761 accgttaaaa tctctttctc acatgctgaa agggaagcaa ggccgtttcc gtcaaaacct
     8821 tcttggtaaa cgtgtcgatt actccggacg ttctgtaatc gttgttggtc ctcatttgaa
     8881 aatgtaccaa tgcggattac cgaaggaaat ggcacttgaa cttttcaaac ctttcgttat
     8941 gaaagaactt gttgaaaaag gtcttgctca caacattaag agtgcgaaac gcaaaattga
     9001 gcgcgtacag cctgaagttt gggatgtact agaatcagtt attaaggagc atccggtact
     9061 gctgaaccgt gcccctacac ttcacagatt aggtatccag gcgtttgaac caacgcttgt
     9121 tgaaggacgc gcaatccgtc ttcacccgct cgtatgtaca gcttacaacg ctgactttga
     9181 cggtgaccaa atggcggttc acgtaccatt atctgctgaa gcacaagctg aagcacgcat
     9241 cttgatgctt gctgctcaaa acatcttgaa ccctaaagat ggtaaaccgg ttgtaacacc
     9301 gtctcaggat atggtactag gtaactatta cctgacactt gagcgtgccg gtgctgtcgg
     9361 tgaaggtatg gtcttcaaga atacagacga agcgcttctt gcttatcaaa acggatatgt
     9421 tcaccttcat acgagagtag ctgttgcagc taactcactt aagaatgtga catttaccga
     9481 agaacagcgc tcaaaattgt taattacaac tgtcggtaag cttgtcttca atgaaattct
     9541 tccggaatca ttcccttaca tgaatgaacc aacgaagagc aacattgaag agaaaacacc
     9601 tgaccgtttc ttcttagaaa aaggtgctga tgttaaagct gttatcgcac agcagccaat
     9661 caatgcgccg tttaaaaaag gcattctggg taaaatcatc gcggaaatct ttaaacgatt
     9721 ccatattacg gaaacgtcta aaatgcttga ccgcatgaaa aacctaggtt tcaaatattc
     9781 aactaaagct ggtattacag ttggggtttc tgacatcgtc gtactcgatg ataaacaaga
     9841 aattcttgag gaagcgcaaa gcaaagttga caacgttatg aagcaattcc gccgcggtct
     9901 tatcactgaa gaagaacgct atgagagagt catctctatc tggagtgctg caaaagacgt
     9961 tatccaaggc aaactgatga aatcacttga tgaactcaac ccgatctaca tgatgagtga
    10021 ctctggtgcc cgtggtaacg catctaactt tacgcagctt gccggaatgc gcggcctgat
    10081 ggccaacccg gctggacgta tcattgagtt gccgatcaaa tcaagtttcc gtgaaggtct
    10141 gacagtattg gagtacttta tctccacaca cggtgcgcgt aaaggtcttg ccgataccgc
    10201 tcttaaaact gctgactcag gttaccttac acgccgtctc gttgacgttg ctcaggatgt
    10261 tatcatccgt gaaactgatt gcggaactga ccgaggcatt cttgctaagc ctcttaaaga
    10321 aggaactgag acaattgagc gcttagaaga acgcttaatc ggacgttttg caagaaaaca
    10381 agtgaagcac cctgaaacag gtgaagtgct tgtgaatgaa aacgaactga tcgatgaaga
    10441 taaagcactg gagattgtcg aagccggcat tgaagaagtg tggatccgtt ctgccttcac
    10501 atgtaatacg cctcatggtg tatgtaaacg atgctatggc cgaaatcttg caactggctc
    10561 cgatgttgaa gtcggtgaag ctgtcggaat cattgctgcc caatcaatcg gtgagcctgg
    10621 tacacagtta acaatgcgta cattccatac aggcggggtt gccggagacg atatcacaca
    10681 gggtcttccg cgtatccaag agcttttcga agcgcgtaat ccgaaaggtc aggcaacaat
    10741 tacagaaatc gacggtacag tcgttgagat caatgaagtt cgtgataagc aacaggaaat
    10801 tgtggttcaa ggcgcagtgg aaacacgttc ttatacggca ccttacaact cccgcctgaa
    10861 agtagcggaa ggagataaaa ttactcgagg ccaagtactg acagaaggtt caatcgatcc
    10921 gaaagaactt cttaaagtga ctgacctgac gactgttcaa gagtatcttc tccatgaggt
    10981 tcaaaaggtt taccgtatgc agggtgttga aatcggtgat aaacacgtag aagtaatggt
    11041 tcgccagatg cttcgcaaag tacgcgtgat tgatgccggt gacactgatg tgcttccagg
    11101 tacattgctt gatattcacc aatttactga agcgaacaaa aaggtattgc ttgaaggcaa
    11161 ccgaccagct acaggccgtc ctgtcttact cggtattaca aaagcatctc ttgaaactga
    11221 ttcattctta tctgctgctt ccttccagga aacaacacgc gtccttacag atgcagcgat
    11281 caaaggtaag cgtgacgagc ttctcggctt gaaagagaat gttatcatcg gtaagcttgt
    11341 tccagcaggt acaggaatga tgaaataccg taaagtaaaa ccagtatcaa atgttcagcc
    11401 gactgacgat atggtcccgg ttgaaggtac ccaccatcac catcaccatc accatcacta
    11461 acatggcatg catcgataga tccggctgct aacaaagccc gaaaggaagc tgagttggct
    11521 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
    11581 ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat
    11641 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
    11701 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
    11761 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
    11821 cggcagtacc ggcataacca agcctatcta cagcatccag ggtgacggtg ccgaggatga
    11881 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
    11941 ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttaattcttg
    12001 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
    12061 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    12121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    12181 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    12241 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    12301 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    12361 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    12421 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
    12481 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    12541 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    12601 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    12661 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    12721 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
    12781 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    12841 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    12901 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    12961 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    13021 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    13081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    13141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    13201 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
