LOCUS       sequence-30685-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Pera EM, De Robertis EM
  TITLE     A direct screen for secreted proteins in Xenopus embryos identifies 
            distinct activities for the Wnt antagonists Crescent and Frzb-1.
  JOURNAL   Mech Dev. 2000 Sep . 96(2):183-95.
  PUBMED    10960783
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev. 
            2000 Sep . 96(2):183-95."
COMMENT     Sequence Label: pCS2 xCrescent-HA
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    135..269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(172..191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     226..245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(302..328)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cattaacctc actaaaggga acaaaagcct ggggataccg ggcccaatgc attggcgccg
       61 cggccgcgaa tnaantnacc gtcccacacc tccccctgaa cctgaaacat aaaatgaatg
      121 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      241 tcatcaatgt atcttatcat gtctggatct acgtaatacg actcactata gttctagatt
      301 atgcgtagtc ggggacgtcg taggggtatg gtcgacactt gtgattcttc catttccgta
      361 cagcgaaatg caggtttttg ttctttttct gccatgcgta ggcattgttc accactacct
      421 tcccctcttg cacttctgca caaatcacat agacgcctgt gcgggtccca cgcatgatat
      481 tctgcacaca ggtagcatcc agatccagcc actgctgaag cctaggaagg acgtctgttt
      541 tgggcaacga gccgtgtttc aggatctcaa gtttggaatc caaatcaaag tcgtaaaggt
      601 tagcggaagt gatgttcttt ttggtgatac ggaccttggc aacaaaatca ttatggcaga
      661 atgtatccag tatctccttg gaagtgctgc cttcttcaag ttcacagtct ctacagct
//
