LOCUS       sequence-30686-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Gupta RK, Arany Z, Seale P, Mepani RJ, Ye L, Conroe HM, Roby YA, 
            Kulaga H, Reed RR, Spiegelman BM
  TITLE     Transcriptional control of preadipocyte determination by Zfp423.
  JOURNAL   Nature. 2010 Mar 25;464(7288):619-23. doi: 10.1038/nature08816. Epub
            2010 Mar 3.
  PUBMED    20200519
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08816"; journalName: "Nature"; date: "2010-03-25- 
            25"; volume: "464"; issue: "7288"; pages: "619-23"
COMMENT     Sequence Label: pMSCVFLAG-ZFP423
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..67
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccttctct ggcgccggaa ttagatctct cgaggccacc atggactaca aagacgatga
       61 cgacaagatg tccaggcgga agcaggcgaa gccgcgatcg gtgaaagttg aagaggggga
      121 ggcctcggac ttctcgctgg cctgggattc ctctgtggca gcagcaggag gcctggaagg
      181 agagccagag tgtgatcgga aaaccagccg tgcgctggaa gacaggaaca gcgtgacaag
      241 tcaagaggag agaaatgagg acgatgaaga cgtggaagat gagtcaattt acacctgcga
      301 tcactgtcag caggacttcg agtctctggc agacctgacg gaccaccggg cccaccgctg
      361 tcctggagat ggtgatgacg acccacagct ctcctgggtg gcttcatctc cctccagcaa
      421 ggatgttgcg tcacctacgc agatgatagg agatggttgt gacctcggcc tcggcgagga
      481 ggaaggcggc accggcctgc cctacccttg ccagttctgc gacaagtcct tcatccgcct
      541 gagctacttg aagaggcatg aacagatcca cagcgacaag ctgccgttca agtgcacctt
      601 ctgcagccgc ctcttcaaac acaagaggag ccgagaccgg cacatcaagc tgcacacggg
      661 cgacaagaag taccactgtc acgagtgcga ggcagctttc tcccgcagcg accacctcaa
      721 gatccacctg aagacccaca gctccagcaa gccgttcaag tgcagcgtgt gcaagcgcgg
      781 cttctcctcc accagct
//
