LOCUS       sequence-306862-        2106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DENV4 47 peptides from NS2B cofactor linked to NS3 full length by 
            GGGGSGGGG linker S135A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Phoo WW, El Sahili A, Zhang Z, Chen MW, Liew CW, Lescar J, Vasudevan
            SG, Luo D
  TITLE     Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic 
            interaction between NS2B and NS3.
  JOURNAL   Antiviral Res. 2020 Oct;182:104900. doi: 
            10.1016/j.antiviral.2020.104900. Epub 2020 Aug 5.
  PUBMED    32763315
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2020-10"; volume: "182:104900. doi"; pages: " 
            10.1016/j.antiviral.2020.104900. Epub 2020 Aug 5"
COMMENT     Sequence Label: gNS2B47NS3 S135A
FEATURES             Location/Qualifiers
     source          1..2106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggg atccgcagac ctgtcactag agaaggccgc caatgtgcag
      121 tgggatgaga tggcagacat aacaggctca agcccaatca tagaagtgaa gcaggatgaa
      181 gatggctctt tctccatacg ggacatcgag gaaaccaata tgataggagg aggaggatca
      241 ggaggaggag gatcaggagc tctgtgggac gtcccctcac ccgctgccac tcagaaagcc
      301 acactgtctg aaggagtgta taggatcatg caaagagggt tgtttgggaa aactcaggtt
      361 ggagtaggga tacacatgga aggtgtattc cacacaatgt ggcacgtgac aagaggatca
      421 gtgatctgcc atgagacagg gagattagag ccatcttggg ctgacgtcag gaatgacatg
      481 atatcatacg gtgggggatg gaggctcgga gacaaatggg acaaagaaga agatgttcag
      541 gtcctagcca tagaaccagg aaaaaatcct aaacatgtcc aaacgaaacc aggccttttc
      601 aagaccctaa ctggagaaat tggagcagta actctggatt tcaaacccgg aacggcgggc
      661 tctcctatca ttaacaagaa agggaaagtt attggactct atggaaatgg agtagttacc
      721 aaatcaggtg attacgtcag tgccataacg caagctgaaa gaattggtga gccagattat
      781 gaagtggatg aggacatttt tcgaaagaaa agattaacca taatggactt acaccccgga
      841 gctggaaaga caaaaagaat tctcccatca atagtcagag aagctttaaa aaggaggctg
      901 cgaactttga ttttggctcc tacgagagtg gtggcagccg agatggaaga ggccctacgc
      961 ggactgccaa tccgttatca gaccccagct gtgaaatcag atcacacagg aagagagatt
     1021 gtggacctca tgtgtcatgc aaccttcaca acgagacttt tgtcatcaac cagggttcca
     1081 aattataacc tcatagtgat ggatgaagca catttcactg acccttgtag tgtcgcggct
     1141 agaggataca tttcaaccag ggtggaaatg ggagaggcag cagccatctt catgactgca
     1201 acccctcctg gatcgataga tcccttcccc cagagcaaca gcccgataga agacatcgaa
     1261 agggaaatcc cagaaaggtc atggaacaca gggttcgact ggataacaga ctaccaaggg
     1321 aaaactgtgt ggtttgttcc cagcataaaa gctggaaatg acattgcaaa ttgtttgcga
     1381 aagtcgggaa agagagtgat ccagttgagc aggaaaacct ttgacacaga gtatccaaaa
     1441 acgaaactca cggactggga ttttgtggta accacagaca tatctgaaat gggggccaat
     1501 ttcagagctg ggagagtgat agaccccagg agatgcctca agccagttat cctaacagat
     1561 gggccagaga gagttattct agcaggtcca atcccagtaa ctccagcaag tgccgctcag
     1621 agaagagggc gaataggtag gaatccagca caagaagatg accaatacgt tttctccgga
     1681 gacccactaa agaatgatga agatcatgcc cactggacag aagcgaagat gttgcttgac
     1741 aatatctaca cccctgaagg gataattcca acattgtttg gtccggaaag ggaaaaaacc
     1801 caagccattg atggagagtt tcgcctcaga ggggaacaaa ggaagacttt tgtggaatta
     1861 atgaggagag gagaccttcc ggtgtggctg agctacaagg tagcttctgc cggtatctct
     1921 tacaaagacc gagaatggtg cttcacaggg gaaaggaaca accaaatttt agaagaaaac
     1981 atggaggttg aaatctggac tagagaggga gaaaagaaaa aactaaggcc aaaatggtta
     2041 gatgcacgtg tgtacgctga ccccatggct ttgaaggatt tcaaggagtt tgccagtgga
     2101 agaaag
//
