LOCUS       sequence-306864-        2100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DENV4 47 peptides from NS2B cofactor linked to NS3 full length by 
            VKTQR linker L30SF31S mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Phoo WW, El Sahili A, Zhang Z, Chen MW, Liew CW, Lescar J, Vasudevan
            SG, Luo D
  TITLE     Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic 
            interaction between NS2B and NS3.
  JOURNAL   Antiviral Res. 2020 Oct;182:104900. doi: 
            10.1016/j.antiviral.2020.104900. Epub 2020 Aug 5.
  PUBMED    32763315
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2020-10"; volume: "182:104900. doi"; pages: " 
            10.1016/j.antiviral.2020.104900. Epub 2020 Aug 5"
COMMENT     Sequence Label: eNS2B47NS3 L30SF31S
FEATURES             Location/Qualifiers
     source          1..2100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggg atccgcagac ctgtcactag agaaggccgc caatgtgcag
      121 tgggatgaga tggcagacat aacaggctca agcccaatca tagaagtgaa gcaggatgaa
      181 gatggctctt tctccatacg ggacatcgag gaaaccaaca tgatagcaca agtgaaaaca
      241 caaagatcag gagctctgtg ggacgtcccc tcacccgctg ccactcagaa agccacactg
      301 tctgaaggag tgtataggat catgcaaaga gggtcttctg ggaaaactca ggttggagta
      361 gggatacaca tggaaggtgt attccacaca atgtggcacg tgacaagagg atcagtgatc
      421 tgccatgaga cagggagatt agagccatct tgggctgacg tcaggaatga catgatatca
      481 tacggtgggg gatggaggct cggagacaaa tgggacaaag aagaagatgt tcaggtccta
      541 gccatagaac caggaaaaaa tcctaaacat gtccaaacga aaccaggcct tttcaagacc
      601 ctaactggag aaattggagc agtaactctg gatttcaaac ccggaacgtc tggctctcct
      661 atcattaaca agaaagggaa agttattgga ctctatggaa atggagtagt taccaaatca
      721 ggtgattacg tcagtgccat aacgcaagct gaaagaattg gtgagccaga ttatgaagtg
      781 gatgaggaca tttttcgaaa gaaaagatta accataatgg acttacaccc cggagctgga
      841 aagacaaaaa gaattctccc atcaatagtc agagaagctt taaaaaggag gctgcgaact
      901 ttgattttgg ctcctacgag agtggtggca gccgagatgg aagaggccct acgcggactg
      961 ccaatccgtt atcagacccc agctgtgaaa tcagatcaca caggaagaga gattgtggac
     1021 ctcatgtgtc atgcaacctt cacaacgaga cttttgtcat caaccagggt tccaaattat
     1081 aacctcatag tgatggatga agcacatttc actgaccctt gtagtgtcgc ggctagagga
     1141 tacatttcaa ccagggtgga aatgggagag gcagcagcca tcttcatgac tgcaacccct
     1201 cctggatcga tagatccctt cccccagagc aacagcccga tagaagacat cgaaagggaa
     1261 atcccagaaa ggtcatggaa cacagggttc gactggataa cagactacca agggaaaact
     1321 gtgtggtttg ttcccagcat aaaagctgga aatgacattg caaattgttt gcgaaagtcg
     1381 ggaaagagag tgatccagtt gagcaggaaa acctttgaca cagagtatcc aaaaacgaaa
     1441 ctcacggact gggattttgt ggtaaccaca gacatatctg aaatgggggc caatttcaga
     1501 gctgggagag tgatagaccc caggagatgc ctcaagccag ttatcctaac agatgggcca
     1561 gagagagtta ttctagcagg tccaatccca gtaactccag caagtgccgc tcagagaaga
     1621 gggcgaatag gtaggaatcc agcacaagaa gatgaccaat acgttttctc cggagaccca
     1681 ctaaagaatg atgaagatca tgcccactgg acagaagcga agatgttgct tgacaatatc
     1741 tacacccctg aagggataat tccaacattg tttggtccgg aaagggaaaa aacccaagcc
     1801 attgatggag agtttcgcct cagaggggaa caaaggaaga cttttgtgga attaatgagg
     1861 agaggagacc ttccggtgtg gctgagctac aaggtagctt ctgccggtat ctcttacaaa
     1921 gaccgagaat ggtgcttcac aggggaaagg aacaaccaaa ttttagaaga aaacatggag
     1981 gttgaaatct ggactagaga gggagaaaag aaaaaactaa ggccaaaatg gttagatgca
     2041 cgtgtgtacg ctgaccccat ggctttgaag gatttcaagg agtttgccag tggaagaaag
//
