LOCUS       sequence-30687-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS2
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(311..316,317..333,334..339))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(366..384)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgtagttcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg
       61 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc
      121 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc
      181 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
      241 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
      301 aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
      361 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      421 gacgtcaggt ggcacttttc ggggaaatgt gcgcgcccgc gttcctgctg gcgctgggcc
      481 tgtttctggc gctggacttc ccgctgttcc gtcagcagct tttcgcccac ggccttgatg
      541 atcgcggcgg ccttggcctg catatcccga ttcaacggcc ccagggcgtc cagaacgggc
      601 ttcaggcgct cccgaaggtc tcgggccgtc tcttgggctt gatcggcctt cttgcgcatc
      661 tcacgcgctc ctgcggcggc ctgtagggca ggctcatacc cctgccgaac cgcttttgtc
      721 agccggtcgg ccacggcttc cggcgtctca acgcgctttg agattcccag cttttcggcc
      781 aatccctgcg gtgcata
//
