LOCUS       sequence-306871-        6596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase reporter containing the downstream Human IGF-1 P2
            Promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry
            EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla 
            RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL
  TITLE     CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers 
            vulnerability to PPARgamma inhibition.
  JOURNAL   Cell Rep. 2021 Feb 23;34(8):108768. doi: 
            10.1016/j.celrep.2021.108768.
  PUBMED    33626346
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-02-23"; volume: "34(8):108768. doi"; pages: " 
            10.1016/j.celrep.2021.108768"
COMMENT     Sequence Label: pIGF1_P2 Promoter-FLuc
FEATURES             Location/Qualifiers
     source          1..6596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..201
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(353..370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(524..1112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(604..623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1283..2074,2075..2143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1906..1925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2144..2248)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2275..2730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2362..2381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2572..2593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2861..2909
                     /note="synthetic polyadenylation signal"
     misc_feature    2923..3014
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2963..2982
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4635..4644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4641..6293
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4707..4725)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6334..6455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6345..6364)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6399..6418
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
       61 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
      121 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
      181 accctaactg acacacattc cacagcggat ccgtcgaccg atgcccttga gagccttcaa
      241 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
      301 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
      361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     1561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     1621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     1681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     1741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     1801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     1861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     1921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     1981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     2281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2701 attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt
     2761 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag
     2821 taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc
     2881 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
     2941 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
     3001 cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagcccg ggctcgagat
     3061 ctccactgat atagagccac taaattgaga gtccatggag ggcaaggagg gtagtcagaa
     3121 aataaatgac tgtttctcca aaagtgatgg ggttgaatgg acctgccact actctgaggt
     3181 tcaaaaaaaa tgctcttctt ataattatag cttccagcat gactccacaa cccttttaat
     3241 agcacccctc acctaacacc ctgtcaggga ctcttcccat gatgctgaaa gtagatgcat
     3301 ctgagagaag tgaagccact caccttgaga ggcttcttgg aaaaagccac atctttgtgc
     3361 cacaagtaac aatatggaaa aacttaatga tcatcttagc atggtttcta ctacttagag
     3421 tgaggatgaa gacttgagcc acaggactgc tgcttttggc aaggctgccg tttggcagtc
     3481 tatcattgac taagtcatgc caaccagggg tcccttggta tattttgcta taaaatagat
     3541 acagtgacac ataagtccct ccaagagatg aagccataat tcctgtgact aaaaataaaa
     3601 taaaagattt ttgccagttt gagcccagtg ccctatacac agaagggatt ctcaacacgt
     3661 gttgatttga tattggactt gaggccaggg actttagaat acaggagtgg tttctatttg
     3721 agccaatttc attcttcgat tctcatattt ttttctgttt ctgttatttt cttctatttg
     3781 tggttgtgtc aactgctgat atgccccagg taaggaatca gtctctctct ctctctctct
     3841 ctctctctct catattgagg aaacaaaaca aaaacaaaac cctgtgatga tatatagtat
     3901 tgtcaaaagc cattttttgg ccatagtaaa ttatttttag tcaaaaatag ggaatgatgt
     3961 tttggttaaa aatattctgc tcaataatga atgcatatac cacctatgga tggccaatat
     4021 tgagagaatt taaattaaca aaacttattt ggccccaaat ctgtactgag cacaatttaa
     4081 cctttgataa ttcaaaagtt ctaccagggt ctcagagtga tatgggatcc cactgtaata
     4141 atagcatcct tcatttccgt agtaaacgtt tctagatatt ttgtctcaat tcattgaaat
     4201 aggaacccat aaagaaaggg ttcagggagg actcctccaa agatctacag tagccagggg
     4261 aataaacaca ggttgttgga tgccgagaca cgctccatcc acaactccct ctgggttctc
     4321 atgtactcta ttggcttctg tgctgggtag tcctgattaa tgacagtcgt ggaatcgtgg
     4381 gagtcaatgc acttctgtcc caccccactc cccttgcaag gatcaaggag gaaacctgac
     4441 cctccctctg tttcttgggc aggtgaagat gcacaccatg tcctcctcgc atctcttcta
     4501 cctggcgctg tgcctgctca ccttcaccag ctctgccacg gctggaccgg agacgctctg
     4561 cggggctgag ctggtggatg ctcttcagtt cgtgtgtgga gacagaagct tggcattccg
     4621 gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
     4681 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
     4741 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
     4801 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
     4861 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
     4921 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     4981 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
     5041 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
     5101 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     5161 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
     5221 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
     5281 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     5341 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     5401 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
     5461 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     5521 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     5581 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     5641 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     5701 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     5761 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     5821 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     5881 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     5941 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     6001 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     6061 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     6121 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     6181 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     6241 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga
     6301 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     6361 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6421 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6481 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     6541 gtggtaaaat cgataaggat ctgaacgatg gagcggagaa tgggcggaac tgggcg
//
