LOCUS       sequence-306873-        8612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-off lentivirus construct all-in-one vector, with Blast 
            resistance, for expression of ALDOA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: 3XFLAG_ALDOA in pCW57_tTA_Blast
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             552..617
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1749..2259
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2178..2196
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2269..2661
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2671..2724
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2725..3342,3343..3348,3349..3384,3385..3387,3388..3423,
                     3424..3426,3427..3462,3463..3471)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3511..3531)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3929..3940)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4145..4378
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4450..4571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4487..4506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4541..4560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4611..4746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4746..4817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4818..4886,4887..5678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5036..5055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5849..6437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6338..6357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6591..6608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6725..6746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6761..6766,6767..6784,6785..6791)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6799..6815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6804..6826
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6823..6839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6823..6839
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6858..6878
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6860..6878
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6906..7132
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7133..7313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7360..7485
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7978..8211
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8396..8440
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca
      601 aggatgacga tgacaaggat ctcaatggtg gtggtggtgg gtcgacgatg ccctaccaat
      661 atccagcact gaccccggag cagaagaagg agctgtctga catcgctcac cgcatcgtgg
      721 cacctggcaa gggcatcctg gctgcagatg agtccactgg gagcattgcc aagcggctgc
      781 agtccattgg caccgagaac accgaagaga accggcgctt ctaccgccag ctgctgctga
      841 cagctgacga ccgcgtgaac ccctgcattg ggggtgtcat cctcttccat gagacactct
      901 accagaaggc ggatgatggg cgtcccttcc cccaagttat caaatccaag ggcggtgttg
      961 tgggcatcaa ggtagacaag ggcgtggtcc ccctggcagg gacaaatggc gagactacca
     1021 cccaagggtt ggatgggctg tctgagcgct gtgcccagta caagaaggac ggagctgact
     1081 tcgccaagtg gcgttgtgtg ctgaagattg gggaacacac cccctcagcc ctcgccatca
     1141 tggaaaatgc caatgttctg gcccgttatg ccagtatctg ccagcagaat ggcattgtgc
     1201 ccatcgtgga gcctgagatc ctccctgatg gggaccatga cttgaagcgc tgccagtatg
     1261 tgaccgagaa ggtgctggct gctgtctaca aggctctgag tgaccaccac atctacctgg
     1321 aaggcacctt gctgaagccc aacatggtca ccccaggcca tgcttgcact cagaagtttt
     1381 ctcatgagga gattgccatg gcgaccgtca cagcgctgcg ccgcacagtg ccccccgctg
     1441 tcactgggat caccttcctg tctggaggcc agagtgagga ggaggcgtcc atcaacctca
     1501 atgccattaa caagtgcccc ctgctgaagc cctgggccct gaccttctcc tacggccgag
     1561 ccctgcaggc ctctgccctg aaggcctggg gcgggaagaa ggagaacctg aaggctgcgc
     1621 aggaggagta tgtcaagcga gccctggcca acagccttgc ctgtcaagga aagtacactc
     1681 cgagcggtca ggctggggct gctgccagcg agtccctctt cgtctctaac cacgcctatt
     1741 aaggatccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     1801 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     1861 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     1921 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     1981 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     2041 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     2101 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     2161 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     2221 ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcaccat ggttcctttg
     2281 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     2341 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     2401 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     2461 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     2521 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     2581 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     2641 tctggttatg tgtgggaggg ccccggtgcc gaaggtagag gttctctcct cacttgtggt
     2701 gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt cataaactct
     2761 gctctggaat tactcaatga agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     2821 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     2881 gatgccctgg caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     2941 gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca
     3001 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     3061 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     3121 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggatca ggagcatcaa
     3181 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     3241 gcaattgagc tgttcgacca tcagggagcc gaacctgcct tccttttcgg cctggaacta
     3301 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt
     3361 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     3421 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccggatg actaagtaag
     3481 gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3541 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3601 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3661 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3721 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3781 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3841 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     3901 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3961 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4021 gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact agaggtacct
     4081 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4141 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     4201 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4261 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4321 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     4381 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4441 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4501 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4561 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4741 gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6661 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6721 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6781 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6841 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6901 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6961 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7021 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7081 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7321 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7381 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7441 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7501 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7561 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7621 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7681 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7741 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7801 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7861 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7921 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7981 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8041 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8101 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8161 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8221 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8281 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8341 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8401 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8461 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8521 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8581 gtttcagacc cacctcccaa ccccgagggg ac
//
