LOCUS       sequence-30688-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..39
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     24..40
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(693..710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(693..709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(702..724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
       61 ctgcagtcat atttcatatg aaaggaggcc atcctatggc ggacacgtta ttgattctgg
      121 gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc ggcctggcct gccttgttga
      181 atgataagtg gcagagtaaa acgtcggtag ttaatgccag catcagcggc gacacctcgc
      241 aacaaggact ggcgcgcctt ccggctctgc tgaaacagca tcagccgcgt tgggtgctgg
      301 ttgaactggg cggcaatgac ggtttgcgtg gttttcagcc acagcaaacc gagcaaacgc
      361 tgcgccagat tttgcaggat gtcaaagccg ccaacgctga accattgtta atgcaaatac
      421 gtctgcctgc aaactatggt cgccgttata atgaagcctt tagcgccatt taccccaaac
      481 tcgccaaaga gtttgatgtt ccgctgctgc ccttttttat ggaagaggtc tacctcaagc
      541 cacaatggat gcaggatgac ggtattcatc ccaaccgcga cgcccagccg tttattgccg
      601 actggatggc gaagcagttg cagcctttag taaatcatga ctcataatct agactccaat
      661 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
      721 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      781 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      841 ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      901 tcagctcatt ttttaaccaa taggccgtac tgcgatgant ggcagggcgg g
//
