LOCUS       sequence-306893-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry
            EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla 
            RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL
  TITLE     CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers 
            vulnerability to PPARgamma inhibition.
  JOURNAL   Cell Rep. 2021 Feb 23;34(8):108768. doi: 
            10.1016/j.celrep.2021.108768.
  PUBMED    33626346
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-02-23"; volume: "34(8):108768. doi"; pages: " 
            10.1016/j.celrep.2021.108768"
COMMENT     Sequence Label: pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..62
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     54..71
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     55..71
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..100
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        81..99
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      120..255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    295..416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(306..325)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     360..379
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             488..721
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(793..1818)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(793..1818)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1819..1872)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(1882..2589)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2157..2176)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2426..2444
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2586..2595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2622..2825)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2651..2671)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2826..3205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3235..3823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3341..3352
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3750..3770
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    3841..3865
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4661..4726)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      4726..4735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4736..4760)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4827..4851)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4896..4914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4932..4950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4968..4986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5004..5022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5040..5058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5076..5094
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5112..5130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(5136..5156)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5174..5291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5557..5601)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5786..6019
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6512..6637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6684..6864
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6865..7091)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(7119..7139)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7119..7137)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7158..7174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7158..7174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7171..7193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7182..7198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7206..7212,7213..7230,7231..7236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7251..7272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7389..7406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7560..8148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7640..7659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8319..9110,9111..9179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8942..8961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9180..9284)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9310..9765)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9447..9468)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9659..9678
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
       61 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag
      121 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
      181 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
      241 agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac atgataagat
      301 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      361 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc
      421 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
      481 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
      541 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
      601 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
      661 agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
      721 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
      781 cttaaaggta ccctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
      841 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
      901 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
      961 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     1021 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     1081 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     1141 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     1201 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     1261 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     1321 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     1381 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     1441 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     1501 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     1561 gctctcgcta aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     1621 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     1681 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     1741 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     1801 gagttcaggc tttttcatgg ggccgggatt ttcctccacg tccccgcatg ttagaagact
     1861 tcccctgccc tctccggagc ccttgtacag ctcgtccatg ccgccggtgg agtggcggcc
     1921 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
     1981 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
     2041 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
     2101 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
     2161 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
     2221 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
     2281 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
     2341 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     2401 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
     2461 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     2521 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
     2581 gctcaccatg gtggcgcagt gggttctcta gttagccaga gagctctgct tatatagacc
     2641 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     2701 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     2761 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     2821 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     2881 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     2941 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     3001 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     3061 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     3121 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     3181 taataactag tcaataatca atgtcaacgc gttcgaaccg cgggaattcc cgatgcgggg
     3241 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc
     3301 gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt
     3361 agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc catggaaagg
     3421 acgtcagctt ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc
     3481 agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga
     3541 aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag
     3601 caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag
     3661 acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat
     3721 gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa
     3781 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcggaa ttcgattatc
     3841 accactttgt acaagaaagc tgggtttata aaaacaaatt tggtgactct ggggtcagag
     3901 gaagagataa agttgttggt ttggcttgta agtgttgtga ctgcgactgt gtgtgggact
     3961 tctttttgga ggtgcatttg tctctgctgc tgtttctgtt ctctgtgggt ttggtgtgag
     4021 gagggtcatc gtttgtggtc agatatttga gaagctccga gcagggacgt ctttgtggct
     4081 tttgctgttg acaaatactc ttcgctttat tgctccatga attctcagtc ttaacaattg
     4141 cagggtttgt tctgatcctg tgattgtgat ttgcatggtt ctgggtactg agaccactgc
     4201 attcattata acttagctga gtgttggctg gtgccagtaa gagcttctta agtagagacg
     4261 gctcttctgc ctcctggggt ggaggggtgc cgtcaggcat ggaggaagga ctagcctcat
     4321 tgtcagtggt cacgtctcca tctgtcagcg catcaaatga gggcaatccg tcttcatcca
     4381 cagggagact gtctagtgtc tctgtgagga ctgctagcaa gtttgcctca ttctcttcat
     4441 ctatcttctc aaatatgttt gaaggctcat tgttgtactg attggatatt atttctgatt
     4501 ggtcactgca ccacttgagt ccacccagaa agctgtctgt atccaagtcg ttcacatcta
     4561 gttcagaaag atcaagttca ggaagatctg ggcaaagagg ctggtcttca ccaaccagag
     4621 cagcacattt caaaattgaa tccatggatg acaatcccaa cttgtcatcg tcatccttgt
     4681 aatcgatatc atgatcttta taatcaccgt catggtcttt gtagtccatg gtggcagcct
     4741 gcttttttgt acaaacttgt ttacgagggt aggaagtggt acggaaagtt ggtataagac
     4801 aaaagtgttg tggaattgct ccaggcgatc tgacggttca ctaaacgagc tctgctttta
     4861 taggcgccca ccgtacacgc ctaaagctta tacgttctct atcactgata gggagtaaac
     4921 tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct ctatcactga
     4981 tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc cttatacgtt
     5041 ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata gggagtaaac
     5101 tcttcatacg ttctctatca ctgataggga gtaaacaact tttctataca aagttgatcc
     5161 gataatcact agtaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta
     5221 tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca atcccccctt
     5281 ttcttttaaa agctagcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct
     5341 gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg
     5401 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     5461 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     5521 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     5581 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     5641 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     5701 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     5761 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     5821 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     5881 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     5941 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     6001 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     6061 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     6121 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct
     6181 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     6241 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     6301 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     6361 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     6421 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     6481 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     6541 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     6601 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     6661 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     6721 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     6781 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     6841 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
     6901 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
     6961 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
     7021 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
     7081 ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg
     7141 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7201 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7261 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7321 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7981 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8221 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8281 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8401 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9301 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     9361 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     9421 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     9481 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     9541 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     9601 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     9661 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     9721 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     9781 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9841 cgaaaggggg at
//
