LOCUS       sequence-306902-        7582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use 
            in bimolecular fluorescence complementation (BiFC) assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Al-Akhrass H, Conway JRW, Poulsen ASA, Paatero I, Kaivola J, Padzik 
            A, Andersen OM, Ivaska J
  TITLE     A feed-forward loop between SorLA and HER3 determines heregulin 
            response and neratinib resistance.
  JOURNAL   Oncogene. 2021 Feb;40(7):1300-1317. doi: 10.1038/s41388-020-01604-5.
            Epub 2021 Jan 8.
  PUBMED    33420373
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-020-01604-5"; journalName: "Oncogene"; date: 
            "2021-02"; volume: "40"; issue: "7"; pages: "1300-1317"
COMMENT     Sequence Label: pDEST-ERBB3-v2
FEATURES             Location/Qualifiers
     source          1..7582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    942..966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4993..5017)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     5240..5261
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5338..5355)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5344..5568
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5656..5673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5827..6415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5907..5926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6586..7377,7378..7446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7209..7228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7447..7551)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag
      961 ttggcaccat gagggcgaac gacgctctgc aggtgctggg cttgcttttc agcctggccc
     1021 ggggctccga ggtgggcaac tctcaggcag tgtgtcctgg gactctgaat ggcctgagtg
     1081 tgaccggcga tgctgagaac caataccaga cactgtacaa gctctacgag aggtgtgagg
     1141 tggtgatggg gaaccttgag attgtgctca cgggacacaa tgccgacctc tccttcctgc
     1201 agtggattcg agaagtgaca ggctatgtcc tcgtggccat gaatgaattc tctactctac
     1261 cattgcccaa cctccgcgtg gtgcgaggga cccaggtcta cgatgggaag tttgccatct
     1321 tcgtcatgtt gaactataac accaactcca gccacgctct gcgccagctc cgcttgactc
     1381 agctcaccga gattctgtca gggggtgttt atattgagaa gaacgataag ctttgtcaca
     1441 tggacacaat tgactggagg gacatcgtga gggaccgaga tgctgagata gtggtgaagg
     1501 acaatggcag aagctgtccc ccctgtcatg aggtttgcaa ggggcgatgc tggggtcctg
     1561 gatcagaaga ctgccagaca ttgaccaaga ccatctgtgc tcctcagtgt aatggtcact
     1621 gctttgggcc caaccccaac cagtgctgcc atgatgagtg tgccgggggc tgctcaggcc
     1681 ctcaggacac agactgcttt gcctgccggc acttcaatga cagtggagcc tgtgtacctc
     1741 gctgtccaca gcctcttgtc tacaacaagc taactttcca gctggaaccc aatccccaca
     1801 ccaagtatca gtatggagga gtttgtgtag ccagctgtcc ccataacttt gtggtggatc
     1861 aaacatcctg tgtcagggcc tgtcctcctg acaagatgga agtagataaa aatgggctca
     1921 agatgtgtga gccttgtggg ggactatgtc ccaaagcctg tgagggaaca ggctctggga
     1981 gccgcttcca gactgtggac tcgagcaaca ttgatggatt tgtgaactgc accaagatcc
     2041 tgggcaacct ggactttctg atcaccggcc tcaatggaga cccctggcac aagatccctg
     2101 ccctggaccc agagaagctc aatgtcttcc ggacagtacg ggagatcaca ggttacctga
     2161 acatccagtc ctggccgccc cacatgcaca acttcagtgt tttttccaat ttgacaacca
     2221 ttggaggcag aagcctctac aaccggggct tctcattgtt gatcatgaag aacttgaatg
     2281 tcacatctct gggcttccga tccctgaagg aaattagtgc tgggcgtatc tatataagtg
     2341 ccaataggca gctctgctac caccactctt tgaactggac caaggtgctt cgggggccta
     2401 cggaagagcg actagacatc aagcataatc ggccgcgcag agactgcgtg gcagagggca
     2461 aagtgtgtga cccactgtgc tcctctgggg gatgctgggg cccaggccct ggtcagtgct
     2521 tgtcctgtcg aaattatagc cgaggaggtg tctgtgtgac ccactgcaac tttctgaatg
     2581 gggagcctcg agaatttgcc catgaggccg aatgcttctc ctgccacccg gaatgccaac
     2641 ccatggaggg cactgccaca tgcaatggct cgggctctga tacttgtgct caatgtgccc
     2701 attttcgaga tgggccccac tgtgtgagca gctgccccca tggagtccta ggtgccaagg
     2761 gcccaatcta caagtaccca gatgttcaga atgaatgtcg gccctgccat gagaactgca
     2821 cccaggggtg taaaggacca gagcttcaag actgtttagg acaaacactg gtgctgatcg
     2881 gcaaaaccca tctgacaatg gctttgacag tgatagcagg attggtagtg attttcatga
     2941 tgctgggcgg cacttttctc tactggcgtg ggcgccggat tcagaataaa agggctatga
     3001 ggcgatactt ggaacggggt gagagcatag agcctctgga ccccagtgag aaggctaaca
     3061 aagtcttggc cagaatcttc aaagagacag agctaaggaa gcttaaagtg cttggctcgg
     3121 gtgtctttgg aactgtgcac aaaggagtgt ggatccctga gggtgaatca atcaagattc
     3181 cagtctgcat taaagtcatt gaggacaaga gtggacggca gagttttcaa gctgtgacag
     3241 atcatatgct ggccattggc agcctggacc atgcccacat tgtaaggctg ctgggactat
     3301 gcccagggtc atctctgcag cttgtcactc aatatttgcc tctgggttct ctgctggatc
     3361 atgtgagaca acaccggggg gcactggggc cacagctgct gctcaactgg ggagtacaaa
     3421 ttgccaaggg aatgtactac cttgaggaac atggtatggt gcatagaaac ctggctgccc
     3481 gaaacgtgct actcaagtca cccagtcagg ttcaggtggc agattttggt gtggctgacc
     3541 tgctgcctcc tgatgataag cagctgctat acagtgaggc caagactcca attaagtgga
     3601 tggcccttga gagtatccac tttgggaaat acacacacca gagtgatgtc tggagctatg
     3661 gtgtgacagt ttgggagttg atgaccttcg gggcagagcc ctatgcaggg ctacgattgg
     3721 ctgaagtacc agacctgcta gagaaggggg agcggttggc acagccccag atctgcacaa
     3781 ttgatgtcta catggtgatg gtcaagtgtt ggatgattga tgagaacatt cgcccaacct
     3841 ttaaagaact agccaatgag ttcaccagga tggcccgaga cccaccacgg tatctggtca
     3901 taaagagaga gagtgggcct ggaatagccc ctgggccaga gccccatggt ctgacaaaca
     3961 agaagctaga ggaagtagag ctggagccag aactagacct agacctagac ttggaagcag
     4021 aggaggacaa cctggcaacc accacactgg gctccgccct cagcctacca gttggaacac
     4081 ttaatcggcc acgtgggagc cagagccttt taagtccatc atctggatac atgcccatga
     4141 accagggtaa tcttggggag tcttgccagg agtctgcagt ttctgggagc agtgaacggt
     4201 gcccccgtcc agtctctcta cacccaatgc cacggggatg cctggcatca gagtcatcag
     4261 aggggcatgt aacaggctct gaggctgagc tccaggagaa agtgtcaatg tgtaggagcc
     4321 ggagcaggag ccggagccca cggccacgcg gagatagcgc ctaccattcc cagcgccaca
     4381 gtctgctgac tcctgttacc ccactctccc cacccgggtt agaggaagag gatgtcaacg
     4441 gttatgtcat gccagataca cacctcaaag gtactccctc ctcccgggaa ggcacccttt
     4501 cttcagtggg tctcagttct gtcctgggta ctgaagaaga agatgaagat gaggagtatg
     4561 aatacatgaa ccggaggaga aggcacagtc cacctcatcc ccctaggcca agttcccttg
     4621 aggagctggg ttatgagtac atggatgtgg ggtcagacct cagtgcctct ctgggcagca
     4681 cacagagttg cccactccac cctgtaccca tcatgcccac tgcaggcaca actccagatg
     4741 aagactatga atatatgaat cggcaacgag atggaggtgg tcctgggggt gattatgcag
     4801 ccatgggggc ctgcccagca tctgagcaag ggtatgaaga gatgagagct tttcaggggc
     4861 ctggacatca ggccccccat gtccattatg cccgcctaaa aactctacgt agcttagagg
     4921 ctacagactc tgcctttgat aaccctgatt actggcatag caggcttttc cccaaggcta
     4981 atgcccagag aacgccaact ttcttgtaca aagtggtcat cgatggtggc ggtggctctg
     5041 gaggtggtgg gtcctccgga aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     5101 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5161 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     5221 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5281 tcggcatgga cgagctgtac aagtaatcta gagggcccgt ttaaacccgc tgatcagcct
     5341 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5401 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5461 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5521 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5581 aaagaaccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7561 ccgaaaagtg ccacctgacg tc
//
