LOCUS       sequence-306903-        7810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use 
            in bimolecular fluorescence complementation (BiFC) assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Al-Akhrass H, Conway JRW, Poulsen ASA, Paatero I, Kaivola J, Padzik 
            A, Andersen OM, Ivaska J
  TITLE     A feed-forward loop between SorLA and HER3 determines heregulin 
            response and neratinib resistance.
  JOURNAL   Oncogene. 2021 Feb;40(7):1300-1317. doi: 10.1038/s41388-020-01604-5.
            Epub 2021 Jan 8.
  PUBMED    33420373
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-020-01604-5"; journalName: "Oncogene"; date: 
            "2021-02"; volume: "40"; issue: "7"; pages: "1300-1317"
COMMENT     Sequence Label: pDEST-ERBB3-v1
FEATURES             Location/Qualifiers
     source          1..7810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    942..966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4993..5017)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5061..5063,5064..5522)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(5103..5124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5364..5383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5566..5583)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5572..5796
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5884..5901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6055..6643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6135..6154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6814..7605,7606..7674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7437..7456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7675..7779)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag
      961 ttggcaccat gagggcgaac gacgctctgc aggtgctggg cttgcttttc agcctggccc
     1021 ggggctccga ggtgggcaac tctcaggcag tgtgtcctgg gactctgaat ggcctgagtg
     1081 tgaccggcga tgctgagaac caataccaga cactgtacaa gctctacgag aggtgtgagg
     1141 tggtgatggg gaaccttgag attgtgctca cgggacacaa tgccgacctc tccttcctgc
     1201 agtggattcg agaagtgaca ggctatgtcc tcgtggccat gaatgaattc tctactctac
     1261 cattgcccaa cctccgcgtg gtgcgaggga cccaggtcta cgatgggaag tttgccatct
     1321 tcgtcatgtt gaactataac accaactcca gccacgctct gcgccagctc cgcttgactc
     1381 agctcaccga gattctgtca gggggtgttt atattgagaa gaacgataag ctttgtcaca
     1441 tggacacaat tgactggagg gacatcgtga gggaccgaga tgctgagata gtggtgaagg
     1501 acaatggcag aagctgtccc ccctgtcatg aggtttgcaa ggggcgatgc tggggtcctg
     1561 gatcagaaga ctgccagaca ttgaccaaga ccatctgtgc tcctcagtgt aatggtcact
     1621 gctttgggcc caaccccaac cagtgctgcc atgatgagtg tgccgggggc tgctcaggcc
     1681 ctcaggacac agactgcttt gcctgccggc acttcaatga cagtggagcc tgtgtacctc
     1741 gctgtccaca gcctcttgtc tacaacaagc taactttcca gctggaaccc aatccccaca
     1801 ccaagtatca gtatggagga gtttgtgtag ccagctgtcc ccataacttt gtggtggatc
     1861 aaacatcctg tgtcagggcc tgtcctcctg acaagatgga agtagataaa aatgggctca
     1921 agatgtgtga gccttgtggg ggactatgtc ccaaagcctg tgagggaaca ggctctggga
     1981 gccgcttcca gactgtggac tcgagcaaca ttgatggatt tgtgaactgc accaagatcc
     2041 tgggcaacct ggactttctg atcaccggcc tcaatggaga cccctggcac aagatccctg
     2101 ccctggaccc agagaagctc aatgtcttcc ggacagtacg ggagatcaca ggttacctga
     2161 acatccagtc ctggccgccc cacatgcaca acttcagtgt tttttccaat ttgacaacca
     2221 ttggaggcag aagcctctac aaccggggct tctcattgtt gatcatgaag aacttgaatg
     2281 tcacatctct gggcttccga tccctgaagg aaattagtgc tgggcgtatc tatataagtg
     2341 ccaataggca gctctgctac caccactctt tgaactggac caaggtgctt cgggggccta
     2401 cggaagagcg actagacatc aagcataatc ggccgcgcag agactgcgtg gcagagggca
     2461 aagtgtgtga cccactgtgc tcctctgggg gatgctgggg cccaggccct ggtcagtgct
     2521 tgtcctgtcg aaattatagc cgaggaggtg tctgtgtgac ccactgcaac tttctgaatg
     2581 gggagcctcg agaatttgcc catgaggccg aatgcttctc ctgccacccg gaatgccaac
     2641 ccatggaggg cactgccaca tgcaatggct cgggctctga tacttgtgct caatgtgccc
     2701 attttcgaga tgggccccac tgtgtgagca gctgccccca tggagtccta ggtgccaagg
     2761 gcccaatcta caagtaccca gatgttcaga atgaatgtcg gccctgccat gagaactgca
     2821 cccaggggtg taaaggacca gagcttcaag actgtttagg acaaacactg gtgctgatcg
     2881 gcaaaaccca tctgacaatg gctttgacag tgatagcagg attggtagtg attttcatga
     2941 tgctgggcgg cacttttctc tactggcgtg ggcgccggat tcagaataaa agggctatga
     3001 ggcgatactt ggaacggggt gagagcatag agcctctgga ccccagtgag aaggctaaca
     3061 aagtcttggc cagaatcttc aaagagacag agctaaggaa gcttaaagtg cttggctcgg
     3121 gtgtctttgg aactgtgcac aaaggagtgt ggatccctga gggtgaatca atcaagattc
     3181 cagtctgcat taaagtcatt gaggacaaga gtggacggca gagttttcaa gctgtgacag
     3241 atcatatgct ggccattggc agcctggacc atgcccacat tgtaaggctg ctgggactat
     3301 gcccagggtc atctctgcag cttgtcactc aatatttgcc tctgggttct ctgctggatc
     3361 atgtgagaca acaccggggg gcactggggc cacagctgct gctcaactgg ggagtacaaa
     3421 ttgccaaggg aatgtactac cttgaggaac atggtatggt gcatagaaac ctggctgccc
     3481 gaaacgtgct actcaagtca cccagtcagg ttcaggtggc agattttggt gtggctgacc
     3541 tgctgcctcc tgatgataag cagctgctat acagtgaggc caagactcca attaagtgga
     3601 tggcccttga gagtatccac tttgggaaat acacacacca gagtgatgtc tggagctatg
     3661 gtgtgacagt ttgggagttg atgaccttcg gggcagagcc ctatgcaggg ctacgattgg
     3721 ctgaagtacc agacctgcta gagaaggggg agcggttggc acagccccag atctgcacaa
     3781 ttgatgtcta catggtgatg gtcaagtgtt ggatgattga tgagaacatt cgcccaacct
     3841 ttaaagaact agccaatgag ttcaccagga tggcccgaga cccaccacgg tatctggtca
     3901 taaagagaga gagtgggcct ggaatagccc ctgggccaga gccccatggt ctgacaaaca
     3961 agaagctaga ggaagtagag ctggagccag aactagacct agacctagac ttggaagcag
     4021 aggaggacaa cctggcaacc accacactgg gctccgccct cagcctacca gttggaacac
     4081 ttaatcggcc acgtgggagc cagagccttt taagtccatc atctggatac atgcccatga
     4141 accagggtaa tcttggggag tcttgccagg agtctgcagt ttctgggagc agtgaacggt
     4201 gcccccgtcc agtctctcta cacccaatgc cacggggatg cctggcatca gagtcatcag
     4261 aggggcatgt aacaggctct gaggctgagc tccaggagaa agtgtcaatg tgtaggagcc
     4321 ggagcaggag ccggagccca cggccacgcg gagatagcgc ctaccattcc cagcgccaca
     4381 gtctgctgac tcctgttacc ccactctccc cacccgggtt agaggaagag gatgtcaacg
     4441 gttatgtcat gccagataca cacctcaaag gtactccctc ctcccgggaa ggcacccttt
     4501 cttcagtggg tctcagttct gtcctgggta ctgaagaaga agatgaagat gaggagtatg
     4561 aatacatgaa ccggaggaga aggcacagtc cacctcatcc ccctaggcca agttcccttg
     4621 aggagctggg ttatgagtac atggatgtgg ggtcagacct cagtgcctct ctgggcagca
     4681 cacagagttg cccactccac cctgtaccca tcatgcccac tgcaggcaca actccagatg
     4741 aagactatga atatatgaat cggcaacgag atggaggtgg tcctgggggt gattatgcag
     4801 ccatgggggc ctgcccagca tctgagcaag ggtatgaaga gatgagagct tttcaggggc
     4861 ctggacatca ggccccccat gtccattatg cccgcctaaa aactctacgt agcttagagg
     4921 ctacagactc tgcctttgat aaccctgatt actggcatag caggcttttc cccaaggcta
     4981 atgcccagag aacgccaact ttcttgtaca aagtggtcat cgatggtggc ggtggctctg
     5041 gaggtggtgg gtcctccgga gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5101 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5161 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     5221 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
     5281 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5341 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5401 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5461 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
     5521 ccgacaagca gtaatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5581 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5641 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5701 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5761 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6781 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7021 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7081 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7801 acctgacgtc
//
