LOCUS       sequence-306905-        7321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use 
            in bimolecular fluorescence complementation (BiFC) assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Al-Akhrass H, Conway JRW, Poulsen ASA, Paatero I, Kaivola J, Padzik 
            A, Andersen OM, Ivaska J
  TITLE     A feed-forward loop between SorLA and HER3 determines heregulin 
            response and neratinib resistance.
  JOURNAL   Oncogene. 2021 Feb;40(7):1300-1317. doi: 10.1038/s41388-020-01604-5.
            Epub 2021 Jan 8.
  PUBMED    33420373
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-020-01604-5"; journalName: "Oncogene"; date: 
            "2021-02"; volume: "40"; issue: "7"; pages: "1300-1317"
COMMENT     Sequence Label: pDEST-ERBB2-v2
FEATURES             Location/Qualifiers
     source          1..7321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    942..966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4732..4756)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4979..5000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5077..5094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5083..5307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5395..5412)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5566..6154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5646..5665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6325..7116,7117..7185))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6948..6967
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7186..7290)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag
      961 ttggcaccat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc
     1021 ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca
     1081 gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag gtggtgcagg
     1141 gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg caggatatcc
     1201 aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga
     1261 ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg gccgtgctag
     1321 acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca ggaggcctgc
     1381 gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg atccagcgga
     1441 acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac aagaacaacc
     1501 agctggctct cacactgata gacaccaacc gctctcgggc ctgccacccc tgttctccga
     1561 tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc ctgacgcgca
     1621 ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac tgctgccatg
     1681 agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc tgcctccact
     1741 tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac aacacagaca
     1801 cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc tgtgtgactg
     1861 cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc tgccccctgc
     1921 acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc agcaagccct
     1981 gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg gcagttacca
     2041 gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg gcatttctgc
     2101 cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca gagcagctcc
     2161 aagtgtttga gactctggaa gagatcacag gttacctata catctcagca tggccggaca
     2221 gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga attctgcaca
     2281 atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac
     2341 tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc tgcttcgtgc
     2401 acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc cacactgcca
     2461 accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag
     2521 ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc cttcggggcc
     2581 aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat gtgaatgcca
     2641 ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg acctgttttg
     2701 gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc ttctgcgtgg
     2761 cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg aagtttccag
     2821 atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt gtggacctgg
     2881 atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc atctctgcgg
     2941 tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc atcaagcgac
     3001 ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg gagctggtgg
     3061 agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc ctgaaagaga
     3121 cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc tacaagggca
     3181 tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa
     3241 acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg gctggtgtgg
     3301 gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg cagctggtga
     3361 cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg
     3421 gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc tacctggagg
     3481 atgtgcggct cgtacacagg gacttggccg ctcggaacgt gctggtcaag agtcccaacc
     3541 atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag acagagtacc
     3601 atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt ctccgccggc
     3661 ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt
     3721 ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg ctggaaaagg
     3781 gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc atggtcaaat
     3841 gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct gaattctccc
     3901 gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg ggcccagcca
     3961 gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg ggggacctgg
     4021 tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac cctgccccgg
     4081 gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt ggcggtgggg
     4141 acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca ctggcaccct
     4201 ccgaaggggc tggctccgat gtatttgatg gtgacctggg aatgggggca gccaaggggc
     4261 tgcaaagcct ccccacacat gaccccagcc ctctacagcg gtacagtgag gaccccacag
     4321 tacccctgcc ctctgagact gatggctacg ttgcccccct gacctgcagc ccccagcctg
     4381 aatatgtgaa ccagccagat gttcggcccc agcccccttc gccccgagag ggccctctgc
     4441 ctgctgcccg acctgctggt gccactctgg aaaggcccaa gactctctcc ccagggaaga
     4501 atggggtcgt caaagacgtt tttgcctttg ggggtgccgt ggagaacccc gagtacttga
     4561 caccccaggg aggagctgcc cctcagcccc accctcctcc tgccttcagc ccagccttcg
     4621 acaacctcta ttactgggac caggacccac cagagcgggg ggctccaccc agcaccttca
     4681 aagggacacc tacggcagag aacccagagt acctgggtct ggacgtgcca gtgccaactt
     4741 tcttgtacaa agtggtcatc gatggtggcg gtggctctgg aggtggtggg tcctccggaa
     4801 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     4861 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4921 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4981 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5041 agtaatctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5281 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgcattaatg
     5341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6001 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6121 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6301 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6361 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6421 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6481 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6541 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6601 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6661 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6721 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6781 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6841 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6901 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6961 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7021 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7081 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7141 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7201 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7261 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7321 c
//
