LOCUS       sequence-306907-       10925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9B(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2346..2360,2361..2366,2367..2383,2384..2390)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2366..2382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2398..2414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(2451..2477,2478..2480,2481..4967,4968..4970,
                     4971..6557)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      7251..7839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7740..7759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa gttggcaaaa atagtttttg
       61 ctatatattt atatataaga aaaaattcaa tactcaagga gataaaaaat ggaaaactta
      121 aacaataatc cacaaaaaat taatcaaata aataagcaaa ctaattttat tggttttgaa
      181 aattttatag aacttgataa tcaagttaat caaactaaag aaaaaagtaa attaagtaag
      241 ataggtaaga aattacccgg aataagcagt caagtctagt gcggccgcat ggcttgttat
      301 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      361 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      421 aaaacaaagt taaacatcat gaggaggtaa cgtcatggta aaagattgga ttcctatttc
      481 tcatgataat tataaacaag tacaaggacc attttatcat ggaactaaag ctaatttagc
      541 aattggtgat ttactaacta caggatttat ttctcatttt gaagatggtc gaattcttaa
      601 acatatttat ttttcagctt taatggaacc agcagtttgg ggagctgaac ttgctatgtc
      661 actatctggt cttgaaggtc gtggttatat atatatagtt gaaccaacag gaccatttga
      721 agatgatcca aatcttacaa ataaaaaatt tcctggtaat ccaacacaat cttatagaac
      781 ttgtgaacct ttaagaattg ttggtgttgt tgaagattgg gaaggacatc ctgttgaatt
      841 aataagagga atgttagatt cattagaaga tttaaaacgt cgtggtttac atgttattga
      901 agattaacct ggcgcgccaa ataatgcccg tgtccctgtt gaatagggac acggtttttt
      961 aacaattttt ttattcctag gccttagtgg ccggtacccg gggatcctta ttactggccg
     1021 ctttctagcc tggatacttg tctggctagt tgcattagag agtcagctag agccctaggg
     1081 ctagctgttt gtgtattcgg agccagtgta gtttttcttt ttactaacga tacgggtaat
     1141 aattgattgc cttttacagc ctgaccttga gacaattgca aaagtctgtc cacagaggtt
     1201 tgacctaata atctaaaatc ttgtttgata gtagtaagtg gaggtatgta gcaactagaa
     1261 tcttctgtat catcataacc cacaactgaa atatcagccc caactctaag tccactttca
     1321 gtaatagctc gcatagctcc taaagccatt tgatcattag caactaacat agcagtaggg
     1381 acaatgcctt catttaacat ttgcattgtt tgctgaaatc ctgacatagc agaccaatct
     1441 ccttctcttt ctgctattgg ttgtatttga tttcttgtta aatacttgtg ccatcctgct
     1501 aatctcaatc tggcacttac tgaacttaag ggacctgcaa gtagtgcaat ttgttgatgg
     1561 cctaatgcaa caaggtgttc aactcctaac cttgttccat cttcgtgaga aaaaattata
     1621 gagtttattg gggtttggtc cgacacatcc aaaaacaatg ctggaacatt agtacatgct
     1681 gcttcaactg caatcgcatc ttggtcatca agaggataat taattataag tccactaaca
     1741 cgttgagcta ataaattatg tactgctgcc ttacaagcct ctacacctga tctctcaacc
     1801 attgatacaa caacgcttgc accaagttga tcagctctcg atttaattgc ggctacaatt
     1861 tgactaggcg catgtaatgc aagtgatgat gtggcaactc caattaaaag tgattgtttc
     1921 cccgcaagtt gctgagcaac gcgatttggt atataattca actctgccat agctgcttca
     1981 accttctctc ttgtttttgc agatacatga cttgcttggt tcacaactct cgaaaccgtt
     2041 tggtagctaa ccccggcata ttcagcaaca tcatacaaag taacaggttt catggatcat
     2101 tcctccatga taaaatttaa atttctgact ttggcaaatc cttttgcctg ttaaaaagaa
     2161 tagcacaaaa attaaaaaaa aagaaacgca aatagcaata gcattaaaaa taattattat
     2221 tatttatttc tttgattaaa atcaaaagcc tttaatgtat tttcttgatc tttcatgcgc
     2281 ttaacggtaa aatccaaggt attaagttca ccttccactt ttctctctct atcttctcta
     2341 gaaaatcata aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg
     2401 tgagcggata acaatttcac acagaattca ttaaagaaga gaaattacat atggataaga
     2461 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
     2521 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
     2581 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
     2641 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
     2701 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
     2761 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
     2821 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
     2881 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
     2941 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
     3001 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
     3061 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
     3121 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
     3181 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
     3241 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     3301 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     3361 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     3421 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
     3481 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
     3541 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
     3601 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
     3661 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
     3721 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
     3781 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
     3841 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
     3901 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
     3961 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
     4021 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
     4081 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
     4141 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
     4201 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
     4261 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
     4321 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
     4381 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
     4441 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
     4501 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
     4561 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
     4621 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
     4681 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
     4741 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
     4801 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
     4861 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
     4921 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac
     4981 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
     5041 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
     5101 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
     5161 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
     5221 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
     5281 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
     5341 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
     5401 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
     5461 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
     5521 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
     5581 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
     5641 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
     5701 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagtc cagacaggcg
     5761 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
     5821 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
     5881 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
     5941 tagggatcac aattctggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
     6001 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
     6061 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
     6121 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
     6181 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
     6241 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
     6301 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
     6361 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
     6421 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
     6481 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
     6541 agctaggagg tgactaataa aagcttgatc agatcccagc ttttttttga agtgcctggc
     6601 agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac
     6661 gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt
     6721 ttccatcata tggctaaacc tttaagtcaa gaagaaagta ctttaattga aagagctact
     6781 gctactatta atagtattcc tattagtgaa gattatagtg ttgctagtgc tgctttaagt
     6841 agtgatggta gaatttttac tggtgttaat gtttatcatt ttactggtgg tccttgtgct
     6901 gaattagttg ttttaggtac tgccgccgct gctgctgctg gtaatctcac ttgtattgtt
     6961 gctattggta atgaaaatag aggtatttta agtccttgtg gtagatgtag acaagtttta
     7021 ttagatttac atcctggtat taaagctatt gttaaagata gtgatggtca acctactgct
     7081 gttggtatta gagaattatt acctagtggt tatgtttggg aaggttaatg acgtcgccgg
     7141 agcggtcgag ttctggaccg accggctcgg gttctcatga ccaaaatccc ttaacgtgag
     7201 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7381 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7621 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc actagatccc
     7921 cggcaagttc atccttaggg ttttcatatc tcttaaaaag tcaatttctg cttgattaat
     7981 ttcttgtgga ttattgtttt tgcggttttt cattttttat ctccttgagt attgaatttt
     8041 ttcttatata taaatatata gcaaaaacta tttttgccaa cttttttaca aaaaattttt
     8101 tattaaaaca cttagggctt tgctaaattc tcttttaaaa gaacttagta aagccctatg
     8161 gatttaagaa ctgattatac ttacgtagta aaaatactac agatattaat agtatagacc
     8221 atatcaaaat cataatcaat ttatatttat ctaaaattgt agtccataca aaatacaaaa
     8281 aaattaggtt tttctaaatt ctattttaaa agaacttagt aacacctaat ctctaaatta
     8341 cttacaaaat tctaaaatta gatttttagt tcttcatatt tctttaaaag ttttttaaga
     8401 aaatcttttt gattttcaaa aatttcatgc atcataaaac tagtaaattt ggattttttt
     8461 ttgtaaaaat cataactttc ttgggtttta agttgaaatc ttaatggttt tattggattt
     8521 tgcctagatt tttttaacgc cggactttct ttatctttta taaaattata agaattttta
     8581 ataccatttt ctattaaata atcttctttt ataagcccct cttgcagtgc agttgctaat
     8641 cttaaatact tatacgcttg agtttttgct acatcaaagt cttttataaa atcaagaaaa
     8701 gttttataac catcaaattt ataatattga ttatctttta tttcttttaa aattttaata
     8761 ttttccaccc tgtggaatat ttcttttttg aaattgtcat ttaacttttc ctttagtgtc
     8821 ttgtaataag aaattttact ttcattaatc attaaatttt gttcaacata agtatctatc
     8881 ttttttgtta aaactttaac aacacgtttt ttttccataa aaattttcct ttatttttgc
     8941 aacattttcc ttaattgata taattgttac tattttagat tttttaatat ccacttaaga
     9001 attaatccct cactagaata ttttttatta tagaattcat atacatgttt ttctaaatct
     9061 ataaatttat caacaattgc tttattatag ggtgtatcta tcttttcttt tcttaataat
     9121 cgtctcatat tctttaaata acaacaaaca ctgccggttt taaacctaaa ttctatgtaa
     9181 tatatttttg aaaaagtata tacattttca accccgttta tttcatactt tacaaaaaca
     9241 ttatttattg gttttctata accatagtaa atgcctaaaa atttgtcccc gtctttcata
     9301 ggatatagat aaagtttttg aatctctgat ttatctaata atttttcaaa aaaaattata
     9361 caccgctttt gtttgttact aactactcta tgcaatgcct ttaatatttt tgtgtgatat
     9421 atatttttat cacctttttt ttctttcagc aggaagatac tttcttttgg aaatttaatt
     9481 ttttgttcat ttattcttgc agccttaagt ccgattttca tatttaatcc ttatatagcc
     9541 tttctattta attcttttaa actcaataaa cttttttggt ttttcattag ttctaataat
     9601 tcgcaataat atgtgcaaaa cactttattg tattctattt tcttttggtt attcaaatat
     9661 tcttttataa ttggctttag aagttcaata tttacttctt tttttagttg ttcaataagg
     9721 atattgaaaa tgtttgtttt taaatcttga taattttgtt gagaattttc tttttttctt
     9781 tcaattgact tctctaattt acgctttata ttgtttaaat cgctatattt atgactttca
     9841 ataataaaat gaggcttaaa tttgtaattt tcgtatattt tttggaaact tgtttctaat
     9901 tgtttagaat tatattcatt tttttcaaat tctttttgag tgttgcatag aatttccttt
     9961 aatttttctt gtttttcttt catacaagat ttagtaaaat gtatattttt attttttagt
    10021 aatttaattt ctattatttt tatgatttta agtattttga ttatattatc tttatcaata
    10081 tttaaattga ataaaattgt aagaaaaatt gaaagaattt ttttacataa aaagttacat
    10141 ttattgaagt aatttcttac ttgatacttt tctatttcat taatctttct ttcttctttt
    10201 atattatttt tattatttaa acactccttt aaatctacac tcccattttt agtaaatcta
    10261 tctttaaggc cccttgaaac tctattttga aatcttaaat cttttttttc ttgaaagtat
    10321 ttatagattt tttggtaaca ttcttttttt gaaaaattaa gttgatagta tatttcggtc
    10381 cccaaattta cccctaagtg tttgtgataa ttagttgtga ctttaaattc tttttctaat
    10441 ttgtaaaggt aattttgcag tgtttttagt ttaacgggtg tttgactatt tcttttaagg
    10501 ttttcattaa agtaatagag tatgtttttt tgtgtgtatt tcttatatgt tttatttatg
    10561 tacgctagtg ttgagataag gactactaat ttatactgta atttattgta gcaagttgtg
    10621 gttttactgg ttttgtttat attagataag ttttccattt atagctcctt gaataccgga
    10681 ttttttatct ataaagtata tatatcacaa ttttgattta aaagtaaatt ctttttttaa
    10741 aaatacaaaa attttttata aattatatag accatgatat tgtattaata ctaattgatt
    10801 ttaatttaag gttgaactaa aataaacata gttttgtaga aaataatttt tcattatttt
    10861 gctacttgat attggatcgt aaaaatatta gggctttaca aagttctttt aaaagagaat
    10921 ttagt
//
