LOCUS       sequence-306917-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitous expression of mtON in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Berry BJ, Trewin AJ, Milliken AS, Baldzizhar A, Amitrano AM, Lim Y, 
            Kim M, Wojtovich AP
  TITLE     Optogenetic control of mitochondrial protonmotive force to impact 
            cellular stress resistance.
  JOURNAL   EMBO Rep. 2020 Apr 3;21(4):e49113. doi: 10.15252/embr.201949113. 
            Epub 2020 Feb 11.
  PUBMED    32043300
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2020-04-3"; volume: "21(4):e49113. doi"; pages: " 
            10.15252/embr.201949113. Epub 2020 Feb 11"
COMMENT     Sequence Label: pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3...
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(13..15,16..726)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(316..335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggtagtag cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagttc atttgcacca ccggcaagct gcccgtgccc
      181 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      481 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      541 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      601 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      721 tacaagtaa
//
