LOCUS       sequence-30693-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS17
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     26..42
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
       61 tcctgcagta ggaggaatta accatgagtt atactgtcgg tacctattta gcggagcggc
      121 ttgtccagat tggtctcaag catcacttcg cagtcgcggg cgactacaac ctcgtccttc
      181 ttgacaacct gcttttgaac aaaaacatgg agcaggttta ttgctgtaac gaactgaact
      241 gcggtttcag tgcagaaggt tatgctcgtg ccaaaggcgc agcagcagcc gtcgttacct
      301 acagcgtcgg tgcgctttcc gcatttgatg ctatcggtgg cgcctatgca gaaaaccttc
      361 cggttatcct gatctccggt gctccgaaca acaatgatca cgctgctggt cacgtgttgc
      421 atcacgctct tggcaaaacc gactatcact atcagttgga aatggccaag aacatcacgg
      481 ccgccgctga agcgatttac accccggaag aagctccggc taaaatcgat cacgtgatta
      541 aaactgctct tcgtgagaag aagccggttt atctcgaaat cgcttgcaac attgcttcca
      601 tgccctgcgc cgctcctgga ccggcaagcg cattgttcaa tgacgaagcc agcgacgaag
      661 cttctttgaa tgcagcggtt gaagaaaccc tgaaattcat cgccaaccgc gacaaagttg
      721 ccgtcctcgt cggcagcaag ctgcgcncag ctggtgctga agaanctgct gtcaaatttg
      781 ctgatgctct cggtggcgca gttgctacca tggctgctgc aaaaagcttc ttcccagaag
      841 aaaacccgca ttacatcggc actcatgggt gagtcagcta tccgggcg
//
