LOCUS       sequence-306946-        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0154:Dendra
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      973..982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaatc gccgcgcaaa gatcgtctgc acgttgggcc cggccacggc cacgtcgtcg
       61 gtactgaccg aactcgtcga cgccggaatg gatgtcgccc ggctgaactt cagccacagc
      121 acccacgccg agcactcggc gctgtacggc atggtccggg agatcgccgc ccagcgtggc
      181 cgcgtggtcg gcgtcctcgc ggacctgcag gggcccaaga tccggctggg gtgtttcgcc
      241 gacggtcccg tcgtatgggc gacgggcgag cacgtcacga tcaccacgga ggactgcccg
      301 ggtgaccacg accgggtctc cacgacctac gcgggcctgt cacaggacgt gcgcgcgggt
      361 gaccggctgt tggtcgacga cgggcgcgtg gatctgcgcg tggtggccgt cgacggtccc
      421 gacatccggt gcgaggtcgt cgacggcggg ccggtcagcg accacaaggg gatatcgctg
      481 cccaacatcc cggtgagcgt tccgccgttg tcggacaagg acatcgagga tctcaagttc
      541 gcgctggagc tcggcgcgga catgatcgcg atgtcgttcg tgcgcgcacc cgaggaggtc
      601 gagctcgcgc acaagatcat ggacgaggtg ggacgccggg tcccggtcat cgcgaaactg
      661 gagaagcctg aggcggtttc ggacctaccc gcgatcgtcg aggcgttcga cggtctcatg
      721 gtcgcgcgcg gcgatctcgg cgtggagatg cccctggagc agatcccgct ggtgcagcgc
      781 cgcgcgatcg ccctgtgccg tcaggcggcc aaacccgtga tcgtggcgac gcagatgctc
      841 gattcgatgg tgtcggaccg ccgcccgaca cgtgccgagg tgtccgacgt cgccaacgcg
      901 gtgttcgaca gggccgatgc ggtgatgctc tcggccgaga cgagtgtggg cgcggatccg
      961 gcgcacgccg tcgccaccat ggcacgcatc gtcgtcgccg ccgagagcgg ttctggccgg
     1021 ggcgagacgc ccgaatcggc gctcgtggag gccgtgaacg gcacggacgc ggtgttcggt
     1081 gacgcgatcg cggtggcggc ctgcgaggtg ggacgccggc tgggcgccgc ggccctgtgc
     1141 tgcttcacgc gcaccggtga cacggcgctg cggttggccc ggcagcggtc accgctgccg
     1201 ctgctggcct tcgcgcacga cgacgaggtg cgggggcgcc tgacgttctc gtggggtgtc
     1261 gagtcggcgg tgctggcacc gacggagctg gccgaatcca tgccgggcga ggtgtcgcgt
     1321 gcgttgctga gcatgggcgc gtgccacccc ggcgatgtcg tggtggtcgt ctccggaagc
     1381 cgaagcggtg tcgcggggta caccgattcg gtccgggtgc tccgtgtcga g
//
