LOCUS       sequence-306951-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0317:Dendra
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(160..171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaaccgcg ctgtggcgct gcgtatcgcg gcatgtggac ttctggggct cggtgctgcc
       61 ctgctgatcg ccgcgttgct gttgaccacc tacaccaagg gcaagatcgc caagatcccg
      121 ctcgacatcg acacgtcgtt ggtcagtgac gggacggcga cggccttcga tccggactcg
      181 ctggtcgccg aacggttcaa gatcgaccgt gacgtgccgg tggccctgca acagcagatg
      241 agcgtcgaag cgccgtccaa cgccgacgtc gtgacgttcc aggtgggcac cacgctgcgc
      301 cgcaccgacc gtcagcagga cgccgggctg ctgctcgcgc tggtggacac cgtcacgatg
      361 aaccggaaca cggccgaggc cgtgtcgagc gagaacaacc cgggtggcgc cgtgcagaag
      421 ccgcgcgcca tcgaggacga gaagccgccc accaacatcg cgctgccgca cgagggcctg
      481 acctaccgct tcccgttcga caccgagaag aagacctatc cgttcttcga cccgatcgcg
      541 cagaaggcgt tcgacgccaa ctacgacggc gaggaagacg tcaacgggct gaccacctac
      601 cggttcgtcc agaacgtcgg ctacgacgcc gacggcaagc tcgccgaccc gatcaagtac
      661 tcctcgctgt acgaggacga cgcagacgcg tcggtcaccg cgcgcgccga ggtgtggggt
      721 gtgccgggcg aaccggacga gtccatcacg atggaccggt tctacgccgc ttcgcgcacg
      781 ttctgggtcg acccagtgtc gggcacgatc gtcaagagcg aagaacacgg ctaccagtac
      841 tacgcacgtg aggccctcaa gcccgaggtc acctacgtgg acttcaaggt caccaccaac
      901 gaggagagcg tcgaatcgca ggttgccgca gcgtccgacg agcgtgaccg catcgcgctg
      961 tggacacgga tcctgccgat cacgttcacc gcgctcggcc tggtgtcgct ggtcggcggc
     1021 gcggtcctcg gttcgttcgc gctgcgcacc gagtccacgc tcatcgatcc gggtctcgac
     1081 accgccgacc acggattctt cgatacgcag ggcatccagg tgccgggtgc ggaggccaag
     1141 accgagaagc tgcccgcgca gcgtccgacc gatctgccac cggaccgacc gatc
//
