LOCUS       sequence-30696-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS100
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(198..289)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(308..335)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(427..513)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     666..683
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cattggaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
       61 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag
      301 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
      361 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
      421 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      481 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
      541 ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
      601 caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc
      661 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagccaagct
      721 tgcatgcctg caggtcgact ctagaggatc cccgggatca ggcgatcttc ttcaagcctt
      781 gcttcttgag ctcttcatca cgcagttcgc ggcgcaggat cttgcccacg ttggtggtcg
      841 gcagcgaatc gcggaactcg atgtggcgcg gtaccttgta gccggtaacg ttg
//
