LOCUS       sequence-30697-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS101
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..279)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(298..325)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(417..503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     656..673
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag aaacgcaaaa
      301 aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta tggcgggcgt
      361 cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc cggcggattt
      421 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      481 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagaccc
      541 cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac
      601 caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt
      661 aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagccaagct tgcatgcctg
      721 caggtcgact ctagaggatc cccgggacta aagcttttct gtcttgacta gtgaaccttc
      781 ggcgtatagt tggtctaatg tatctttgaa gaattttgat gctttggcac gctttatttt
      841 aaaagttggc gtcacaacat catcctcgag agttaaaggc tcaagtcc
//
