LOCUS       sequence-306998-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0372:Dendra
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcttcgg acctgttttc ccaagtggtc aattccgggc cgggatcgtt cctggccaag
       61 cagctgggcg tgccgcagcc cgagacgttg cgccgctacc gccccggtga cccgccgctg
      121 gcgggctcgc tgctgatcgg cggatccggc cgcgtggccg aacccctgcg caccgcattg
      181 gccgacgact acaacctggt gtccaacaac atcggtggcc ggtgggccga ttcgttcggc
      241 ggcgtggtgt tcgacgccac gggcatcacc gaggccgagg gcctcaagga gctctacacg
      301 ttcttcacgc cgctgctgcg caacctcgcg ccgtgtgcgc gggtggtcgt cgtcggcacc
      361 acgcccgcgg aggccggcag cgtccacgca caggtggtgc agcgggcgct ggaaggtttc
      421 acgcgctcgc tgggcaagga gctgcgccgt ggcgcgaccg tctccctggt ctatctctcg
      481 gccgacgcca agccgggcgc cacgggtctc gagtcgacca tgcggttcat cctgtcggcc
      541 aagtcggcct acgtcgacgg tcaggtgttc cgcgtcggcg ccgccgattc gactccgccc
      601 gccgactggg acaagccgct cgacggcaag gtcgccgtgg tcaccggcgc ggcccgcggt
      661 atcggcgcga cgatcgccga ggtgttcgcg cgtgacggcg cgaccgtggt ggcgatcgac
      721 gtcgacggtg cggccgagga tctcaagcgc gtcgccgaca aggtgggcgg caccgcactc
      781 acgctcgacg tgaccgccga cgacgccgtc gacaagatca ccgcgcacgt caccgaacac
      841 catggcggca aggtcgacat cctcgtcaac aacgcgggta tcacgcgcga caagctgctc
      901 gccaacatgg acgagaagcg ctgggacgcg gtgatcgccg tcaacctgct cgcgccgcag
      961 cgcctcaccg aaggcctggt gggcaacgga accatcggcg agggcggccg ggtgatcggc
     1021 ctgtcgtcga tggcgggcat cgcgggcaac cgcggccaga ccaattacgc caccaccaag
     1081 gccgggatga tcgggctggc cgaggcgctg gcgcccgtgc tggccgacaa gggcatcacg
     1141 atcaacgccg tcgcacccgg cttcatcgag accaagatga ccgaggccat cccgctcgcc
     1201 acgcgtgagg tgggcaggcg gctcaactcg ctgttccagg gcggccagcc cgtcgacgtg
     1261 gccgagttga tcgcgtactt cgccagcccc gcctcgaacg cggtgacggg caacaccatc
     1321 cgcgtgtgcg gccaggcgat gctgggggcg
//
