LOCUS       sequence-30700-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS104
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(51..56,57..74,75..81)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atcgtttagg cacccaaggc tttacacttt
       61 atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca gaattcaaaa
      121 gatcttttaa gaaggagata tacatatgaa gaaggtttgg cttaaccgtt atcccgcgga
      181 cgttccgacg gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc
      241 ggtcgcgcgc tacgccgatc aacctgcgtt tgtgaatatg ggggaggtaa tgaccttccg
      301 caagctggaa gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa
      361 gaaaggcgat cgcgttgcgt tgatgatgcc taatttattg caatatccgg tggcgctgtt
      421 tggcattttg cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata ccccgcgtga
      481 gcttgagcat cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc
      541 tcacacactg gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat
      601 gggcgatcag ctatctacgg caaagggcac ggtagtcaat ttcgttgtta aatacatcaa
      661 gcgtttggtg ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa
      721 cggctaccgg atgcagtacg tcaaacccga actggtgccg gaagatttag cttttctgca
      781 atacaccggc ggcaccactg gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct
      841 ggcgaacctg gaacaggtta acgcgacc
//
