LOCUS       sequence-307003-        1833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0406:Dendra
FEATURES             Location/Qualifiers
     source          1..1833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      478..487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgagccact acaagagcaa tgtccgtgat caggtattca acctgttcga ggttttcggt
       61 gtggacaagg tgctcggcgc tgacaagttc agcgacctgg atgccgacac cgcccgcgag
      121 atgctcaccg agatcgcgcg cctggccgag ggcccgatcg ccgaatcctt cgtcgagggt
      181 gaccgcaatc ccccggtgtt cgatcccgaa acccataccg tcacgctgcc cgagggcttc
      241 aagaagtcga tgcgtgcgct gttcgacggc ggctgggaca aggtcggcct cgcagagcac
      301 ctcggcggca tcccgatgcc gcgtgcactg cagtgggccc tgatcgagca catcctcggc
      361 gccaaccctg cggcctacat gtacgccatg ggcccgggca tgagcgagat cttctacaac
      421 aacggcaccg acgagcagaa gaagtgggcc accatcgccg ccgagcgcgg ctggggcgcc
      481 accatggtgc tgaccgaacc cgacgcgggc tccgacgtcg gcgcgggccg caccaaggca
      541 gttcagcagc ctgacggcac ctggcacatc gagggtgtga agcgattcat cacctcggcc
      601 gactccgacg acctgttcga gaacatcatg cacctggtgc tggcccgccc cgagggcgcc
      661 ggaccgggca ccaagggtct gtcgctgttc ttcgtgccga agttccactt cgaccacgag
      721 accggtgaga tcggtgagcg caacggcgtt ttcgtcacca acgtcgagca caagatgggc
      781 ctgaaggtct cggccacgtg tgagctgtcg ctgggtcagc acggcatccc cgccgtcggc
      841 tggctggtgg gcgaggtgca caacggcatc gcgcagatgt tcgacgtgat cgagcaggcg
      901 cgaatgatgg tgggtaccaa ggccatcgcc acgctgtcga ccggttacct caatgcgctc
      961 gagtacgcca aggagcgtgt ccagggcgcc gacatgacgc agatgaccga caagaccgca
     1021 ccgcgcgtga ccatcacgca ccacccggat gtgcgtcgca gcctgatgac ccagaaggcc
     1081 tacgccgagg gtctgcgtgc gatctacctg tacaccgcga ccttccagga cgccgaggtg
     1141 gcccaggccg tgcacggcgt cgacggcgac ctcgccgcac gcgtcaacga tctgctgctg
     1201 ccgatcgtca agggcttcgg ctcggagacc gcctacgcca agctcaccga gagcctgcag
     1261 accctcggtg gttccggctt cctgcaggac tacccgatcg agcagtacat ccgcgactcg
     1321 aagatcgact cgctgtacga gggcaccact gccatccagg cgcaggactt cttcttccgc
     1381 aagatcatcc gtgacaaggg tcaggcactg gcctacgtcg cgggcgagat cgagcagttc
     1441 atcaagaacg agaacggcaa cggccgcctg aagaccgagc gcgaactgct cgccaccgcg
     1501 ctcgccgacg tccagggcat ggccgcttca ctgaccggct acctgatggc cgcgcaggag
     1561 gatgccgcct cgatctacaa ggtgggcctc ggctcggtcc ggttcctgat ggcggtcggc
     1621 gatctgctgt ccggctggct gctggcacgc caggccgcgg tggccatcga gaagctcgac
     1681 gcaggcgcca ccggtgccga caagtccttc tacgagggca agatcgccgc ggcgtcgttc
     1741 ttcgccaaga acatgctgcc gctgctgacc agcacgcgcc agatcatcga gaacctcgac
     1801 aacgacgtca tggagttgga cgaggcggcg ttc
//
