LOCUS       sequence-307027-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector; Unmodifed CRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71102
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..1048
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 cactctgtgg tctcaaatgt ccaatttact gaccgtacac caaaatttgc ctgcattacc
       61 ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
      121 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg
      181 gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct
      241 tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct
      301 aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact
      361 ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct
      421 agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
      481 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc
      541 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat
      601 ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct
      661 gggggtaact aaactggtcg agcgatggat ttccgtttct ggtgtagctg atgatccgaa
      721 taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca
      781 gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc
      841 taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc
      901 cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg
      961 gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat
     1021 ggtgcgcctg ctggaagatg gcgattaggc tttgagacca cgaagtg
//
