LOCUS       sequence-307032-        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pTetO_105
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..717)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ttatttgtac aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa
       61 gaccatgtgg tctctctttt cgtttggatc tttggataag gcagattgag tggataagta
      121 atggttgtct ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc
      181 agctaattga acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc
      241 attcttttgt ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt
      301 gtgacctaaa atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac
      361 taaggtatca ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa
      421 aaaaatagtt ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg
      481 tttcatatga tctgggtatc tcgcaaaaca ttgaacacca taaccgaaag tagtgactaa
      541 ggttggccat ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc
      601 gtaagtagca tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc
      661 atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt tagacattgt
      721 tttatgacag atttgtaatt aaaacttaga ttagattgct atgctttctt tctaatgagc
      781 aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa ctgaaacttg agaaattctc
      841 tatcaatgat agaggaagac cgtttattaa cttaaatatc aatgggaggt catcgaaaga
      901 gaaaaaaatc aaaaaaaaaa attttcaaga aaaagaaacg tgataaaaat ttttattgcc
      961 tttttcgacg aagaaaaaga aacgaggcgg tctctttttt cttttccaaa cctttagtac
     1021 gggtaattaa cgacacccta gaggaagaaa gaggggaaat ttagtatgct gtgcttgggt
     1081 gttttgaagt ggtacggcga tgcgcggagt ccgagaaaat ctggaagagt aaaaaaggag
     1141 tagaaacatt ttgaagctat ggtgtgtgc
//
