LOCUS       sequence-307033-       12469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet-MYO10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Jacquemet G, Stubb A, Saup R, Miihkinen M, Kremneva E, Hamidi H, 
            Ivaska J
  TITLE     Filopodome Mapping Identifies p130Cas as a Mechanosensitive 
            Regulator of Filopodia Stability.
  JOURNAL   Curr Biol. 2019 Jan 21;29(2):202-216.e7. doi: 
            10.1016/j.cub.2018.11.053. Epub 2019 Jan 10.
  PUBMED    30639111
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.11.053"; journalName: "Curr Biol"; date: 
            "2019-01-21- 21"; volume: "29"; issue: "2"; pages: "202-216.e7"
COMMENT     Sequence Label: mScarlet-MYO10
FEATURES             Location/Qualifiers
     source          1..12469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             932..949
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             953..985
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(989..997,998..1012)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1030..1725
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1163..1181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1431..1450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1741..1767
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1786..1803
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      2067..2076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2191..2200
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6963..6972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8060..8077)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8066..8290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8336..8764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8423..8442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8633..8654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8773..8793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8778..9107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8958..9093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9020..9039
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9174..9968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9228..9247)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9838..9857
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10142..10263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10179..10198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10233..10252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10312..10328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10312..10328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10325..10347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10336..10352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10360..10366,10367..10384,10385..10390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10405..10426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10543..10560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10714..11302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10794..10813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11473..12264,12265..12333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12096..12115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12334..12438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag
      961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtaccaat
     1021 gttactagta tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
     1081 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1201 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac caagcacccc
     1261 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1321 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     1381 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     1441 cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     1501 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac
     1561 ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac
     1621 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     1681 tccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatcc
     1741 tacccctatg atgtgcctga ctatgccgga cacggacacg gccacctggt gcccaggggc
     1801 agcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccatgga taacttcttc
     1861 accgagggaa cacgggtctg gctgagagaa aatggccagc attttccaag tactgtaaat
     1921 tcctgtgcag aaggcatcgt cgtcttccgg acagactatg gtcaggtatt cacttacaag
     1981 cagagcacaa ttacccacca gaaggtgact gctatgcacc ccacgaacga ggagggcgtg
     2041 gatgacatgg cgtccttgac agagctccat ggcggctcca tcatgtataa cttattccag
     2101 cggtataaga gaaatcaaat atatacctac atcggctcca tcctggcctc cgtgaacccc
     2161 taccagccca tcgccgggct gtacgagcct gccaccatgg agcagtacag ccggcgccac
     2221 ctgggcgagc tgcccccgca catcttcgcc atcgccaacg agtgctaccg ctgcctgtgg
     2281 aagcgctacg acaaccagtg catcctcatc agtggtgaaa gtggggcagg taaaaccgaa
     2341 agcactaaat tgatcctcaa gtttctgtca gtcatcagtc aacagtcttt ggaattgtcc
     2401 ttaaaggaga agacatcctg tgttgaacga gctattcttg aaagcagccc catcatggaa
     2461 gctttcggca atgcgaagac cgtgtacaac aacaactcta gtcgctttgg gaagtttgtt
     2521 cagctgaaca tctgtcagaa aggaaatatt cagggcggga gaattgtaga ttatttatta
     2581 gaaaaaaacc gagtagtaag gcaaaatccc ggggaaagga attatcacat attttatgca
     2641 ctgctggcag ggctggaaca tgaagaaaga gaagaatttt atttatctac gccagaaaac
     2701 taccactact tgaatcagtc tggatgtgta gaagacaaga caatcagtga ccaggaatcc
     2761 tttagggaag ttattacggc aatggacgtg atgcagttca gcaaggagga agttcgggaa
     2821 gtgtcgaggc tgcttgctgg tatactgcat cttgggaaca tagaatttat cactgctggt
     2881 ggggcacagg tttccttcaa aacagctttg ggcagatctg cggagttact tgggctggac
     2941 ccaacacagc tcacagatgc tttgacccag agatcaatgt tcctcagggg agaagagatc
     3001 ctcacgcctc tcaatgttca acaggcagta gacagcaggg actccctggc catggctctg
     3061 tatgcgtgct gctttgagtg ggtaatcaag aagatcaaca gcaggatcaa aggcaatgag
     3121 gacttcaagt ctattggcat cctcgacatc tttggatttg aaaactttga ggttaatcac
     3181 tttgaacagt tcaatataaa ctatgcaaac gagaaacttc aggagtactt caacaagcat
     3241 attttttctt tagaacaact agaatatagc cgggaaggat tagtgtggga agatattgac
     3301 tggatagaca atggagaatg cctggacttg attgagaaga aacttggcct cctagccctt
     3361 atcaatgaag aaagccattt tcctcaagcc acagacagca ccttattgga gaagctacac
     3421 agtcagcatg cgaataacca cttttatgtg aagcccagag ttgcagttaa caattttgga
     3481 gtgaagcact atgctggaga ggtgcaatat gatgtccgag gtatcttgga gaagaacaga
     3541 gatacatttc gagatgacct tctcaatttg ctaagagaaa gccgatttga ctttatctac
     3601 gatctttttg aacatgtttc aagccgcaac aaccaggata ccttgaaatg tggaagcaaa
     3661 catcggcggc ctacagtcag ctcacagttc aaggactcac tgcattcctt aatggcaacg
     3721 ctaagctcct ctaatccttt ctttgttcgc tgtatcaagc caaacatgca gaagatgcca
     3781 gaccagtttg accaggcggt tgtgctgaac cagctgcggt actcagggat gctggagact
     3841 gtgagaatcc gcaaagctgg gtatgcggtc cgaagaccct ttcgggactt ttacaaaagg
     3901 tataaagtgc tgatgaggaa tctggctctg cctgaggacg tccgagggaa gtgcacgagc
     3961 ctgctgcagc tctatgatgc ctccaacagc gagtggcagc tggggaagac caaggtcttt
     4021 cttcgagaat ccttggaaca gaaactggag aagcggaggg aagaggaagt gagccacgcg
     4081 gccatggtga ttcgggccca tgtcttgggc ttcttagcac gaaaacaata cagaaaggtc
     4141 ctttattgtg tggtgataat acagaagaat tacagagcat tccttctgag gaggagattt
     4201 ttgcacctga aaaaggcagc catagttttc cagaagcaac tcagaggtca gattgctcgg
     4261 agagtttaca gacaattgct ggcagagaaa agggagcaag aagaaaagaa gaaacaggaa
     4321 gaggaagaaa agaagaaacg ggaggaagaa gaaagagaaa gagagagaga gcgaagagaa
     4381 gccgagctcc gcgcccagca ggaagaagaa acgaggaagc agcaagaact cgaagccttg
     4441 cagaagagcc agaaggaagc tgaactgacc cgtgaactgg agaaacagaa ggaaaataag
     4501 caggtggaag agatcctccg tctggagaaa gaaatcgagg acctgcagcg catgaaggag
     4561 cagcaggagc tgtcgctgac cgaggcttcc ctgcagaagc tgcaggagcg gcgggaccag
     4621 gagctccgca ggctggagga ggaagcgtgc agggcggccc aggagttcct cgagtccctc
     4681 aatttcgacg agatcgacga gtgtgtccgg aatatcgagc ggtccctgtc ggtgggaagc
     4741 gaattttcca gcgagctggc tgagagcgca tgcgaggaga agcccaactt caacttcagc
     4801 cagccctacc cagaggagga ggtcgatgag ggcttcgaag ccgacgacga cgccttcaag
     4861 gactccccca accccagcga gcacggccac tcagaccagc gaacaagtgg catccggacc
     4921 agcgatgact cttcagagga ggacccatac atgaacgaca cggtggtgcc caccagcccc
     4981 agtgcggaca gcacggtgct gctcgcccca tcagtgcagg actccgggag cctacacaac
     5041 tcctccagcg gcgagtccac ctactgcatg ccccagaacg ctggggactt gccctcccca
     5101 gacggcgact acgactacga ccaggatgac tatgaggacg gtgccatcac ttccggcagc
     5161 agcgtgacct tctccaactc ctacggcagc cagtggtccc ccgactaccg ctgctctgtg
     5221 gggacctaca acagctcggg tgcctaccgg ttcagctctg agggggcgca gtcctcgttt
     5281 gaagatagtg aagaggactt tgattccagg tttgatacag atgatgagct ttcataccgg
     5341 cgtgactctg tgtacagctg tgtcactctg ccgtatttcc acagctttct gtacatgaaa
     5401 ggtggcctga tgaactcttg gaaacgccgc tggtgcgtcc tcaaggatga aaccttcttg
     5461 tggttccgct ccaagcagga ggccctcaag caaggctggc tccacaaaaa aggggggggc
     5521 tcctccacgc tgtccaggag aaattggaag aagcgctggt ttgtcctccg ccagtccaag
     5581 ctgatgtact ttgaaaacga cagcgaggag aagctcaagg gcaccgtaga agtgcgaacg
     5641 gcaaaagaga tcatagataa caccaccaag gagaatggga tcgacatcat tatggccgat
     5701 aggactttcc acctgattgc agagtcccca gaagatgcca gccagtggtt cagcgtgctg
     5761 agtcaggtcc acgcgtccac ggaccaggag atccaggaga tgcatgatga gcaggcaaac
     5821 ccacagaatg ctgtgggcac cttggatgtg gggctgattg attctgtgtg tgcctctgac
     5881 agccctgata gacccaactc gtttgtgatc atcacggcca accgggtgct gcactgcaac
     5941 gccgacacgc cggaggagat gcaccactgg ataaccctgc tgcagaggtc caaaggggac
     6001 accagagtgg agggccagga attcatcgtg agaggatggt tgcacaaaga ggtgaagaac
     6061 agtccgaaga tgtcttcact gaaactgaag aaacggtggt ttgtactcac ccacaattcc
     6121 ctggattact acaagagttc agagaagaac gcgctcaaac tggggaccct ggtcctcaac
     6181 agcctctgct ctgtcgtccc cccagatgag aagatattca aagagacagg ctactggaac
     6241 gtcaccgtgt acgggcgcaa gcactgttac cggctctaca ccaagctgct caacgaggcc
     6301 acccggtggt ccagtgccat tcaaaacgtg actgacacca aggccccgat cgacaccccc
     6361 acccagcagc tgattcaaga tatcaaggag aactgcctga actcggatgt ggtggaacag
     6421 atttacaagc ggaacccgat ccttcgatac acccatcacc ccttgcactc cccgctcctg
     6481 ccccttccgt atggggacat aaatctcaac ttgctcaaag acaaaggcta taccaccctt
     6541 caggatgagg ccatcaagat attcaattcc ctgcagcaac tggagtccat gtctgaccca
     6601 attccaataa tccagggcat cctacagaca gggcatgacc tgcgacctct gcgggacgag
     6661 ctgtactgcc agcttatcaa acagaccaac aaagtgcccc accccggcag tgtgggcaac
     6721 ctgtacagct ggcagatcct gacatgcctg agctgcacct tcctgccgag tcgagggatt
     6781 ctcaagtatc tcaagttcca tctgaaaagg atacgggaac agtttccagg aaccgagatg
     6841 gaaaaatacg ctctcttcac ttacgaatct cttaagaaaa ccaaatgccg agagtttgtg
     6901 ccttcccgag atgaaataga agctctgatc cacaggcagg aaatgacatc cacggtctat
     6961 tgccatggcg gcggctcctg caagatcacc atcaactccc acaccactgc tggggaggtg
     7021 gtggagaagc tgatccgagg cctggccatg gaggacagca ggaacatgtt tgctttgttt
     7081 gaatacaacg gccacgtcga caaagccatt gaaagtcgaa ccgtcgtagc tgatgtctta
     7141 gccaagtttg aaaagctggc tgccacatcc gaggttgggg acctgccatg gaaattctac
     7201 ttcaaacttt actgcttcct ggacacagac aacgtgccaa aagacagtgt ggagtttgca
     7261 tttatgtttg aacaggccca cgaagcggtt atccatggcc accatccagc cccggaagaa
     7321 aacctccagg ttcttgctgc cctgcgactc cagtatctgc agggggatta tactctgcac
     7381 gctgccatcc cacctctcga agaggtttat tccctgcaga gactcaaggc ccgcatcagc
     7441 cagtcaacca aaaccttcac cccttgtgaa cggctggaga agaggcggac gagcttccta
     7501 gaggggaccc tgaggcggag cttccggaca ggatccgtgg tccggcagaa ggtcgaggag
     7561 gagcagatgc tggacatgtg gattaaggaa gaagtctcct ctgctcgagc cagtatcatt
     7621 gacaagtgga ggaaatttca gggaatgaac caggaacagg ccatggccaa gtacatggcc
     7681 ttgatcaagg agtggcctgg ctatggctcg acgctgtttg atgtggagtg caaggaaggt
     7741 ggcttccctc aggaactctg gttgggtgtc agcgcggacg ccgtctccgt ctacaagcgt
     7801 ggagagggaa gaccactgga agtcttccag tatgaacaca tcctctcttt tggggcaccc
     7861 ctggcgaata cgtataagat cgtggtcgat gagagggagc tgctctttga aaccagtgag
     7921 gtggtggatg tggccaagct catgaaagcc tacatcagca tgatcgtgaa gaagcgctac
     7981 agcacgacac gctccgccag cagccagggc agctccaggt gaaggcggtc tagagggccc
     8041 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     8101 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8161 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8221 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8281 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8761 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9121 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     9181 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     9241 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     9301 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     9361 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     9421 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     9481 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     9541 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     9601 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     9661 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     9721 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9781 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9841 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9901 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9961 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
    10021 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10081 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10141 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10261 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    10321 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10381 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10441 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10501 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    11161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11581 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    12121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    12181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12421 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
