LOCUS       sequence-307038-        9354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged MYO10 with MyTH/FERM deletion in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Miihkinen M, Gronloh MLB, Popovic A, Vihinen H, Jokitalo E, Goult 
            BT, Ivaska J, Jacquemet G
  TITLE     Myosin-X and talin modulate integrin activity at filopodia tips.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109716. doi: 
            10.1016/j.celrep.2021.109716.
  PUBMED    34525374
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109716. doi"; pages: " 
            10.1016/j.celrep.2021.109716"
COMMENT     Sequence Label: EGFP-MYO10 Delta-F
FEATURES             Location/Qualifiers
     source          1..9354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1345..1371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1372..1389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1390..1407
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1671..1680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1795..1804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6142..6263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6179..6198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6233..6252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6270..6725)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6407..6428)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6619..6638
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6752..6856
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6858..7215
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6858..7049
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6881..6901)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7066..7201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7250..8044
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7250..8044
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7304..7323)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7914..7933
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8232..8251)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8276..8323
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8652..9240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9141..9160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atcctacccc tatgatgtgc ctgactatgc ccaccaccac
     1381 caccaccacc tggtgcccag gggcagcaga tctcgagctc aagcttcgaa ttctgcagtc
     1441 gacggtacca tggataactt cttcaccgag ggaacacggg tctggctgag agaaaatggc
     1501 cagcattttc caagtactgt aaattcctgt gcagaaggca tcgtcgtctt ccggacagac
     1561 tatggtcagg tattcactta caagcagagc acaattaccc accagaaggt gactgctatg
     1621 caccccacga acgaggaggg cgtggatgac atggcgtcct tgacagagct ccatggcggc
     1681 tccatcatgt ataacttatt ccagcggtat aagagaaatc aaatatatac ctacatcggc
     1741 tccatcctgg cctccgtgaa cccctaccag cccatcgccg ggctgtacga gcctgccacc
     1801 atggagcagt acagccggcg ccacctgggc gagctgcccc cgcacatctt cgccatcgcc
     1861 aacgagtgct accgctgcct gtggaagcgc tacgacaacc agtgcatcct catcagtggt
     1921 gaaagtgggg caggtaaaac cgaaagcact aaattgatcc tcaagtttct gtcagtcatc
     1981 agtcaacagt ctttggaatt gtccttaaag gagaagacat cctgtgttga acgagctatt
     2041 cttgaaagca gccccatcat ggaagctttc ggcaatgcga agaccgtgta caacaacaac
     2101 tctagtcgct ttgggaagtt tgttcagctg aacatctgtc agaaaggaaa tattcagggc
     2161 gggagaattg tagattattt attagaaaaa aaccgagtag taaggcaaaa tcccggggaa
     2221 aggaattatc acatatttta tgcactgctg gcagggctgg aacatgaaga aagagaagaa
     2281 ttttatttat ctacgccaga aaactaccac tacttgaatc agtctggatg tgtagaagac
     2341 aagacaatca gtgaccagga atcctttagg gaagttatta cggcaatgga cgtgatgcag
     2401 ttcagcaagg aggaagttcg ggaagtgtcg aggctgcttg ctggtatact gcatcttggg
     2461 aacatagaat ttatcactgc tggtggggca caggtttcct tcaaaacagc tttgggcaga
     2521 tctgcggagt tacttgggct ggacccaaca cagctcacag atgctttgac ccagagatca
     2581 atgttcctca ggggagaaga gatcctcacg cctctcaatg ttcaacaggc agtagacagc
     2641 agggactccc tggccatggc tctgtatgcg tgctgctttg agtgggtaat caagaagatc
     2701 aacagcagga tcaaaggcaa tgaggacttc aagtctattg gcatcctcga catctttgga
     2761 tttgaaaact ttgaggttaa tcactttgaa cagttcaata taaactatgc aaacgagaaa
     2821 cttcaggagt acttcaacaa gcatattttt tctttagaac aactagaata tagccgggaa
     2881 ggattagtgt gggaagatat tgactggata gacaatggag aatgcctgga cttgattgag
     2941 aagaaacttg gcctcctagc ccttatcaat gaagaaagcc attttcctca agccacagac
     3001 agcaccttat tggagaagct acacagtcag catgcgaata accactttta tgtgaagccc
     3061 agagttgcag ttaacaattt tggagtgaag cactatgctg gagaggtgca atatgatgtc
     3121 cgaggtatct tggagaagaa cagagataca tttcgagatg accttctcaa tttgctaaga
     3181 gaaagccgat ttgactttat ctacgatctt tttgaacatg tttcaagccg caacaaccag
     3241 gataccttga aatgtggaag caaacatcgg cggcctacag tcagctcaca gttcaaggac
     3301 tcactgcatt ccttaatggc aacgctaagc tcctctaatc ctttctttgt tcgctgtatc
     3361 aagccaaaca tgcagaagat gccagaccag tttgaccagg cggttgtgct gaaccagctg
     3421 cggtactcag ggatgctgga gactgtgaga atccgcaaag ctgggtatgc ggtccgaaga
     3481 ccctttcggg acttttacaa aaggtataaa gtgctgatga ggaatctggc tctgcctgag
     3541 gacgtccgag ggaagtgcac gagcctgctg cagctctatg atgcctccaa cagcgagtgg
     3601 cagctgggga agaccaaggt ctttcttcga gaatccttgg aacagaaact ggagaagcgg
     3661 agggaagagg aagtgagcca cgcggccatg gtgattcggg cccatgtctt gggcttctta
     3721 gcacgaaaac aatacagaaa ggtcctttat tgtgtggtga taatacagaa gaattacaga
     3781 gcattccttc tgaggaggag atttttgcac ctgaaaaagg cagccatagt tttccagaag
     3841 caactcagag gtcagattgc tcggagagtt tacagacaat tgctggcaga gaaaagggag
     3901 caagaagaaa agaagaaaca ggaagaggaa gaaaagaaga aacgggagga agaagaaaga
     3961 gaaagagaga gagagcgaag agaagccgag ctccgcgccc agcaggaaga agaaacgagg
     4021 aagcagcaag aactcgaagc cttgcagaag agccagaagg aagctgaact gacccgtgaa
     4081 ctggagaaac agaaggaaaa taagcaggtg gaagagatcc tccgtctgga gaaagaaatc
     4141 gaggacctgc agcgcatgaa ggagcagcag gagctgtcgc tgaccgaggc ttccctgcag
     4201 aagctgcagg agcggcggga ccaggagctc cgcaggctgg aggaggaagc gtgcagggcg
     4261 gcccaggagt tcctcgagtc cctcaatttc gacgagatcg acgagtgtgt ccggaatatc
     4321 gagcggtccc tgtcggtggg aagcgaattt tccagcgagc tggctgagag cgcatgcgag
     4381 gagaagccca acttcaactt cagccagccc tacccagagg aggaggtcga tgagggcttc
     4441 gaagccgacg acgacgcctt caaggactcc cccaacccca gcgagcacgg ccactcagac
     4501 cagcgaacaa gtggcatccg gaccagcgat gactcttcag aggaggaccc atacatgaac
     4561 gacacggtgg tgcccaccag ccccagtgcg gacagcacgg tgctgctcgc cccatcagtg
     4621 caggactccg ggagcctaca caactcctcc agcggcgagt ccacctactg catgccccag
     4681 aacgctgggg acttgccctc cccagacggc gactacgact acgaccagga tgactatgag
     4741 gacggtgcca tcacttccgg cagcagcgtg accttctcca actcctacgg cagccagtgg
     4801 tcccccgact accgctgctc tgtggggacc tacaacagct cgggtgccta ccggttcagc
     4861 tctgaggggg cgcagtcctc gtttgaagat agtgaagagg actttgattc caggtttgat
     4921 acagatgatg agctttcata ccggcgtgac tctgtgtaca gctgtgtcac tctgccgtat
     4981 ttccacagct ttctgtacat gaaaggtggc ctgatgaact cttggaaacg ccgctggtgc
     5041 gtcctcaagg atgaaacctt cttgtggttc cgctccaagc aggaggccct caagcaaggc
     5101 tggctccaca aaaaaggggg gggctcctcc acgctgtcca ggagaaattg gaagaagcgc
     5161 tggtttgtcc tccgccagtc caagctgatg tactttgaaa acgacagcga ggagaagctc
     5221 aagggcaccg tagaagtgcg aacggcaaaa gagatcatag ataacaccac caaggagaat
     5281 gggatcgaca tcattatggc cgataggact ttccacctga ttgcagagtc cccagaagat
     5341 gccagccagt ggttcagcgt gctgagtcag gtccacgcgt ccacggacca ggagatccag
     5401 gagatgcatg atgagcaggc aaacccacag aatgctgtgg gcaccttgga tgtggggctg
     5461 attgattctg tgtgtgcctc tgacagccct gatagaccca actcgtttgt gatcatcacg
     5521 gccaaccggg tgctgcactg caacgccgac acgccggagg agatgcacca ctggataacc
     5581 ctgctgcaga ggtccaaagg ggacaccaga gtggagggcc aggaattcat cgtgagagga
     5641 tggttgcaca aagaggtgaa gaacagtccg aagatgtctt cactgaaact gaagaaacgg
     5701 tggtttgtac tcacccacaa ttccctggat tactacaaga gttcagagaa gaacgcgctc
     5761 aaactgggga ccctggtcct caacagcctc tgctctgtcg tccccccaga tgagaagata
     5821 ttcaaagaga caggctactg gaacgtcacc gtgtacgggc gcaagcactg ttaccggctc
     5881 tacaccaagc tgctcaacga ggccacccgg tggtccagtg ccattcaaaa cgtgactgac
     5941 accaaggccc cgatcgatta ataagcactt tcactgaaga ctgaagatca gcattggcag
     6001 cattgggtgg tgaagacgat gcagtctaga taactgatca taatcagcca taccacattt
     6061 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6241 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     6301 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     6361 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     6421 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6481 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     6541 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6601 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6661 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6721 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6841 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     6901 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6961 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7021 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7081 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7141 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7201 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7261 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     7321 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     7381 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     7441 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     7501 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     7561 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7621 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7681 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7741 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     7801 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7861 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7921 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7981 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8041 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8101 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8161 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     8221 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     8281 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     8341 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     8401 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     8461 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     8521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
