LOCUS       sequence-307039-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain 
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Miihkinen M, Gronloh MLB, Popovic A, Vihinen H, Jokitalo E, Goult 
            BT, Ivaska J, Jacquemet G
  TITLE     Myosin-X and talin modulate integrin activity at filopodia tips.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109716. doi: 
            10.1016/j.celrep.2021.109716.
  PUBMED    34525374
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109716. doi"; pages: " 
            10.1016/j.celrep.2021.109716"
COMMENT     Sequence Label: mScarlet-I-Myo10 Delta-F
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1308
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(746..764)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1014..1033
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1324..1350
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1351..1368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1369..1386
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1650..1659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1774..1783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6121..6242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6158..6177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6212..6231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6249..6704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6386..6407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6598..6617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6731..6835
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6837..7194
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6837..7028
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6860..6880)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7045..7180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7229..8023
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7229..8023
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7283..7302)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7893..7912
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8211..8230)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8255..8302
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8631..9219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9120..9139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
      661 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      781 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac
      841 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
      901 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
      961 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
     1021 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
     1081 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
     1141 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
     1201 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
     1261 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
     1321 tcctacccct atgatgtgcc tgactatgcc caccaccacc accaccacct ggtgcccagg
     1381 ggcagcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccat ggataacttc
     1441 ttcaccgagg gaacacgggt ctggctgaga gaaaatggcc agcattttcc aagtactgta
     1501 aattcctgtg cagaaggcat cgtcgtcttc cggacagact atggtcaggt attcacttac
     1561 aagcagagca caattaccca ccagaaggtg actgctatgc accccacgaa cgaggagggc
     1621 gtggatgaca tggcgtcctt gacagagctc catggcggct ccatcatgta taacttattc
     1681 cagcggtata agagaaatca aatatatacc tacatcggct ccatcctggc ctccgtgaac
     1741 ccctaccagc ccatcgccgg gctgtacgag cctgccacca tggagcagta cagccggcgc
     1801 cacctgggcg agctgccccc gcacatcttc gccatcgcca acgagtgcta ccgctgcctg
     1861 tggaagcgct acgacaacca gtgcatcctc atcagtggtg aaagtggggc aggtaaaacc
     1921 gaaagcacta aattgatcct caagtttctg tcagtcatca gtcaacagtc tttggaattg
     1981 tccttaaagg agaagacatc ctgtgttgaa cgagctattc ttgaaagcag ccccatcatg
     2041 gaagctttcg gcaatgcgaa gaccgtgtac aacaacaact ctagtcgctt tgggaagttt
     2101 gttcagctga acatctgtca gaaaggaaat attcagggcg ggagaattgt agattattta
     2161 ttagaaaaaa accgagtagt aaggcaaaat cccggggaaa ggaattatca catattttat
     2221 gcactgctgg cagggctgga acatgaagaa agagaagaat tttatttatc tacgccagaa
     2281 aactaccact acttgaatca gtctggatgt gtagaagaca agacaatcag tgaccaggaa
     2341 tcctttaggg aagttattac ggcaatggac gtgatgcagt tcagcaagga ggaagttcgg
     2401 gaagtgtcga ggctgcttgc tggtatactg catcttggga acatagaatt tatcactgct
     2461 ggtggggcac aggtttcctt caaaacagct ttgggcagat ctgcggagtt acttgggctg
     2521 gacccaacac agctcacaga tgctttgacc cagagatcaa tgttcctcag gggagaagag
     2581 atcctcacgc ctctcaatgt tcaacaggca gtagacagca gggactccct ggccatggct
     2641 ctgtatgcgt gctgctttga gtgggtaatc aagaagatca acagcaggat caaaggcaat
     2701 gaggacttca agtctattgg catcctcgac atctttggat ttgaaaactt tgaggttaat
     2761 cactttgaac agttcaatat aaactatgca aacgagaaac ttcaggagta cttcaacaag
     2821 catatttttt ctttagaaca actagaatat agccgggaag gattagtgtg ggaagatatt
     2881 gactggatag acaatggaga atgcctggac ttgattgaga agaaacttgg cctcctagcc
     2941 cttatcaatg aagaaagcca ttttcctcaa gccacagaca gcaccttatt ggagaagcta
     3001 cacagtcagc atgcgaataa ccacttttat gtgaagccca gagttgcagt taacaatttt
     3061 ggagtgaagc actatgctgg agaggtgcaa tatgatgtcc gaggtatctt ggagaagaac
     3121 agagatacat ttcgagatga ccttctcaat ttgctaagag aaagccgatt tgactttatc
     3181 tacgatcttt ttgaacatgt ttcaagccgc aacaaccagg ataccttgaa atgtggaagc
     3241 aaacatcggc ggcctacagt cagctcacag ttcaaggact cactgcattc cttaatggca
     3301 acgctaagct cctctaatcc tttctttgtt cgctgtatca agccaaacat gcagaagatg
     3361 ccagaccagt ttgaccaggc ggttgtgctg aaccagctgc ggtactcagg gatgctggag
     3421 actgtgagaa tccgcaaagc tgggtatgcg gtccgaagac cctttcggga cttttacaaa
     3481 aggtataaag tgctgatgag gaatctggct ctgcctgagg acgtccgagg gaagtgcacg
     3541 agcctgctgc agctctatga tgcctccaac agcgagtggc agctggggaa gaccaaggtc
     3601 tttcttcgag aatccttgga acagaaactg gagaagcgga gggaagagga agtgagccac
     3661 gcggccatgg tgattcgggc ccatgtcttg ggcttcttag cacgaaaaca atacagaaag
     3721 gtcctttatt gtgtggtgat aatacagaag aattacagag cattccttct gaggaggaga
     3781 tttttgcacc tgaaaaaggc agccatagtt ttccagaagc aactcagagg tcagattgct
     3841 cggagagttt acagacaatt gctggcagag aaaagggagc aagaagaaaa gaagaaacag
     3901 gaagaggaag aaaagaagaa acgggaggaa gaagaaagag aaagagagag agagcgaaga
     3961 gaagccgagc tccgcgccca gcaggaagaa gaaacgagga agcagcaaga actcgaagcc
     4021 ttgcagaaga gccagaagga agctgaactg acccgtgaac tggagaaaca gaaggaaaat
     4081 aagcaggtgg aagagatcct ccgtctggag aaagaaatcg aggacctgca gcgcatgaag
     4141 gagcagcagg agctgtcgct gaccgaggct tccctgcaga agctgcagga gcggcgggac
     4201 caggagctcc gcaggctgga ggaggaagcg tgcagggcgg cccaggagtt cctcgagtcc
     4261 ctcaatttcg acgagatcga cgagtgtgtc cggaatatcg agcggtccct gtcggtggga
     4321 agcgaatttt ccagcgagct ggctgagagc gcatgcgagg agaagcccaa cttcaacttc
     4381 agccagccct acccagagga ggaggtcgat gagggcttcg aagccgacga cgacgccttc
     4441 aaggactccc ccaaccccag cgagcacggc cactcagacc agcgaacaag tggcatccgg
     4501 accagcgatg actcttcaga ggaggaccca tacatgaacg acacggtggt gcccaccagc
     4561 cccagtgcgg acagcacggt gctgctcgcc ccatcagtgc aggactccgg gagcctacac
     4621 aactcctcca gcggcgagtc cacctactgc atgccccaga acgctgggga cttgccctcc
     4681 ccagacggcg actacgacta cgaccaggat gactatgagg acggtgccat cacttccggc
     4741 agcagcgtga ccttctccaa ctcctacggc agccagtggt cccccgacta ccgctgctct
     4801 gtggggacct acaacagctc gggtgcctac cggttcagct ctgagggggc gcagtcctcg
     4861 tttgaagata gtgaagagga ctttgattcc aggtttgata cagatgatga gctttcatac
     4921 cggcgtgact ctgtgtacag ctgtgtcact ctgccgtatt tccacagctt tctgtacatg
     4981 aaaggtggcc tgatgaactc ttggaaacgc cgctggtgcg tcctcaagga tgaaaccttc
     5041 ttgtggttcc gctccaagca ggaggccctc aagcaaggct ggctccacaa aaaagggggg
     5101 ggctcctcca cgctgtccag gagaaattgg aagaagcgct ggtttgtcct ccgccagtcc
     5161 aagctgatgt actttgaaaa cgacagcgag gagaagctca agggcaccgt agaagtgcga
     5221 acggcaaaag agatcataga taacaccacc aaggagaatg ggatcgacat cattatggcc
     5281 gataggactt tccacctgat tgcagagtcc ccagaagatg ccagccagtg gttcagcgtg
     5341 ctgagtcagg tccacgcgtc cacggaccag gagatccagg agatgcatga tgagcaggca
     5401 aacccacaga atgctgtggg caccttggat gtggggctga ttgattctgt gtgtgcctct
     5461 gacagccctg atagacccaa ctcgtttgtg atcatcacgg ccaaccgggt gctgcactgc
     5521 aacgccgaca cgccggagga gatgcaccac tggataaccc tgctgcagag gtccaaaggg
     5581 gacaccagag tggagggcca ggaattcatc gtgagaggat ggttgcacaa agaggtgaag
     5641 aacagtccga agatgtcttc actgaaactg aagaaacggt ggtttgtact cacccacaat
     5701 tccctggatt actacaagag ttcagagaag aacgcgctca aactggggac cctggtcctc
     5761 aacagcctct gctctgtcgt ccccccagat gagaagatat tcaaagagac aggctactgg
     5821 aacgtcaccg tgtacgggcg caagcactgt taccggctct acaccaagct gctcaacgag
     5881 gccacccggt ggtccagtgc cattcaaaac gtgactgaca ccaaggcccc gatcgattaa
     5941 taagcacttt cactgaagac tgaagatcag cattggcagc attgggtggt gaagacgatg
     6001 cagtctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6061 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6121 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6181 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6241 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6301 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6361 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6421 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6481 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6541 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6601 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6661 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6721 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6781 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     6841 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6901 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7021 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7141 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     7201 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     7261 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7321 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7381 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     7441 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7501 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7561 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7621 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7681 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7741 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7801 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7861 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7921 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7981 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     8041 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     8101 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8161 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     8221 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     8281 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     8341 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     8401 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     8461 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     8521 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8761 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9301 gattctgtgg ataaccgtat taccgccatg cat
//
