LOCUS       sequence-307054-        2765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter for P. tricornutum; uLoop specific syntax; AC 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_AC_p45582
FEATURES             Location/Qualifiers
     source          1..2765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1239..1827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1319..1338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1918..2709)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
       61 gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc atgcctgcaa gtcgactcta
      121 gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
      181 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
      241 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      301 atgggctgcc tgtatcgagt ggtgattttg tgccgagctc attactcgca tccattctca
      361 ggctgtctcg tctcgtctca ggtctccgga ggggatacag ggcgattgtg aatggcggga
      421 cgataggcag ccggattgct ctactgcgtt ctgcggcccg aatattggcg tttcattgtc
      481 cctttacggg ggtcacaaaa ctattgcgat actcccagca gagttttgaa taattgcacc
      541 tggaatgcct cacgtcccag ccgcacttca ggatctacgc tacttgcaca gtgtttttct
      601 ctatacttca ggatagcgac attgttttga taatcttttc gatcgattcg gacattagaa
      661 tgtgtctgtc ctaaactttc gaaatgtccc tcttcattag gaagagtaga gtgaccgatt
      721 cgaacgcgga actacagtac gctccgcctc aaaatagtaa ccccagggct gtatcgtttg
      781 cggcagcgca gtcatacgct gtgcgaacac cgctgtcgac acagccccca cgcaggaaga
      841 tacgagaagc acacccgctc tcgtcgctct agtcgctcta gtctctccga ccagatagtc
      901 aacacaccgc ttctttctac tgggcatcac tacccttgat attctgcttc ctgttgctgc
      961 tacggttttc ttttggcaat cctcactcca tccagttctg ttactgtgta ttgcgtcagt
     1021 gcagcctttc ttcgattcgt cgttccagaa tctaccttgc cattcgtcgc ccgacgccag
     1081 tagcaacgca gctaccaatg cgagaccacc aggatacata gattaccaca actccgagcc
     1141 cttccaccca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtgattatta
     1921 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
     1981 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     2041 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     2101 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     2161 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     2221 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     2281 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     2341 ctcgaagata ccagcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     2401 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     2461 gagaatctca ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     2521 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
     2581 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     2641 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     2701 ttccctcatg cgaaacgatc ctcatcctgt ctcttgatca gatattgatc ccctgcgcca
     2761 tcaga
//
