LOCUS       sequence-307055-        2489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter for P. tricornutum; uLoop specific syntax; AC 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_AC_pNub
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1016..1604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1096..1115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1695..2486)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaacgatcct catcctgtct cttgatcaga tattgatccc ctgcgccatc agatccttgg
       61 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
      121 agctggcaat tccggttcgc ttgctaagct tgcatgcctg caagtcgact ctagaggagc
      181 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
      241 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
      301 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
      361 gcctgtatcg agtggtgatt ttgtgccgag ctcattactc gcatccattc tcaggctgtc
      421 tcgtctcgtc tcaggtctcc ggagaaaagt tgaagatctg cagcaacctg gacaatttgt
      481 cgttcttaac atgttatacc gctttcatgc ttgaagaatt ttttgaaaaa tcaatgtcat
      541 atgcatcgtt ctagccgatc ctcttactct agaggaaaca ctagattttc gcagcaccca
      601 ccatgcattg atgtgtaatc gttatgagat gccaggcaag taaggccagc caacaaacgg
      661 ctaattgaat ttcggtttgg ctggaagtat gaaatgttcc gtttgagctt ctgtcacgct
      721 aaggataacg ttaaagtaat ctgcgtctac gtgaaaggcc acgtcaaaca gccggtatgt
      781 gattcccatg attcgctgct ttccaacttc tcgctgcttt tcacaccaga aaaacatact
      841 ttacgttgac aaagaggggc aaaagactgc atcaatgcga gaccaccagg atacatagat
      901 taccacaact ccgagccctt ccacccacag aatcagggga taacgcagga aagaacatgt
      961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     1681 attctaggtg attattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
     1741 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
     1801 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
     1861 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
     1921 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
     1981 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
     2041 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
     2101 atgtcgatcg tggctggctc gaagatacca gcaagaatgt cattgcgctg ccattctcca
     2161 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     2221 gtgacttcta cagcgcggag aatctcactc tctccagggg aagccgaagt ttccaaaagg
     2281 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     2341 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     2401 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     2461 acttcggcga tcaccgcttc cctcatgcg
//
