LOCUS       sequence-307057-        2541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator for P. tricornutum; uLoop specific syntax; EF 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_EF_tNub
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1483..2274
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2365..2541,1..412)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
       61 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      481 tgcgttatcc cctgattctg tgggtggaag ggctcggagt tgtggtaatc tatgtatcct
      541 ggtggtctcg agcgcctagg ttaaacgatt ctgacaggat ttcgaattcc aatgagcttc
      601 actttcaagg aggtgttcca agtcaaaagg ttgagtggct gtgaagagtc tatacacatt
      661 ccggtgaata gatggttgtc cactgtaaga acgagcctgt ttcgggtgta gtgtttccct
      721 cttcaatgtc ttgctcccgt aaggagcgaa gcatgtcaaa tctcatcccg acccttcagc
      781 ggcactattc tcgcgaggag ctgatctccg gagtgccttg atggccttag caaatgagca
      841 acgagacgca gcgaaaacta cccgcaaatc tctgtatatg cctctcatat cagtaaaata
      901 aaccaaatcc ttatctcggc tacaaatgtt tacctcttct gtaagattgt tcgattgact
      961 ttgctggcag cgaactcttg ttgtaccata aagcttccct caatccattt gaaaatattc
     1021 atagtctcac tctgcaagcg gagacctgag acgagacgag acagcctgag aatggatgcg
     1081 agtaatgagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     1141 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     1201 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     1261 gattgtaacg atgacagagc gttgctgcct gtgatcaccg ctcctctaga gtcgacttgc
     1321 aggcatgcaa gcttagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     1381 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     1441 ggatcaatat ctgatcaaga gacaggatga ggatcgtttc gcatgaggga agcggtgatc
     1501 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1561 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     1621 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1681 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gtgagattct ccgcgctgta
     1741 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1801 ctgcaatttg gagaatggca gcgcaatgac attcttgctg gtatcttcga gccagccacg
     1861 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1921 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1981 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2041 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2101 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2161 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2221 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataataatca
     2281 cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2521 gtagccgtag ttaggccacc a
//
