LOCUS       sequence-307058-        2219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator for P. tricornutum; uLoop specific syntax; EF 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_EF_t45582
FEATURES             Location/Qualifiers
     source          1..2219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(751..1339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(831..850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1430..2219,1..2))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atgcgaaacg atcctcatcc tgtctcttga tcagatattg atcccctgcg ccatcagatc
       61 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
      121 gccccagctg gcaattccgg ttcgcttgct aagcttgcat gcctgcaagt cgactctaga
      181 ggagcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
      241 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
      301 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
      361 gggctgcctg tatcgagtgg tgattttgtg ccgagctcat tactcgcatc cattctcagg
      421 ctgtctcgtc tcgtctcagg tctccgctta gcaaatagaa agttattagt gcagcgtcgt
      481 tattcgattg cactagtaaa aatctcatgt tcgcaacagc atgtactaac agtaggcttt
      541 gtgcttgttt taagcaaaaa cggaaccact gtagtaatcc ataagcatgt cgttgtatca
      601 gccaaaggcg ctcgagacca ccaggataca tagattacca caactccgag cccttccacc
      661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1381 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtgattat tatttgccga
     1441 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     1501 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     1561 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     1621 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     1681 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     1741 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     1801 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     1861 taccagcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     1921 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     1981 cactctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     2041 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     2101 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     2161 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctc
//
