LOCUS       sequence-30706-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pKS1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(45..50,51..68,69..75)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    83..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      717..803
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttgagctta tcatcgataa gcttccgatg gcgcgccgag aggctttaca ctttatgctt
       61 ccggctcgta taatgtgtgg aattgtgagc ggataacaat tgaattcaaa ggaggccatc
      121 ctatggcgga cacgttattg attctgggtg atagcctgag cgccgggtat cgaatgtctg
      181 ccagcgcggc ctggcctgcc ttgttgaatg ataagtggca gagtaaaacg tcggtagtta
      241 atgccagcat cagcggcgac acctcgcaac aaggactggc gcgccttccg gctctgctga
      301 aacagcatca gccgcgttgg gtgctggttg aactgggcgg caatgacggt ttgcgtggtt
      361 ttcagccaca gcaaaccgag caaacgctgc gccagatttt gcaggatgtc aaagccgcca
      421 acgctgaacc attgttaatg caaatacgtc tgcctgcaaa ctatggtcgc cgttataatg
      481 aagcctttag cgccatttac cccaaactcg ccaaagagtt tgatgttccg ctgctgccct
      541 tttttatgga agaggtctac ctcaagccac aatggatgca ggatgacggt attcatccca
      601 accgcgacgc ccagccgttt attgccgact ggatggcgaa gcagttgcag cctttagtaa
      661 atcatgactc ataagagctc caattcgccc tatagtgaga cgcgtgctag aggcatcaaa
      721 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      781 acgctctcct gagtaggaca aatccgccgc cctagaccta ggggatatat tccgcttcct
      841 taattaactc caggccggcc tacgcgttta aacttccggt taacgccatg agcggcctca
      901 tttcttattc tgagttacaa cagtccgcac cgctgccggt agctccttcc cggtgggcgc
      961 ggggcatgac tatcgtcgcc gcacttatga ctgtcttta
//
