LOCUS       sequence-307060-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0526-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1436..1450,1451..1456,1457..1473,1474..1480)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1456..1472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1488..1504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1523..1534
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1541..1567,1568..1570,1571..4057,4058..4060,
                     4061..5647)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5817..6608
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6731..7319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7220..7239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        10929..11004
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcgcgccaa ataatgcccg tgtccctgtt gaatagggac acggtttttt aacaattttt
       61 ttattcctag gccttagtgg ccggtacccg gggatcctta ttactggccg ctttctagcc
      121 tggatacttg tctggctagt tgcattagag agtcagctag agccctaggg ctagctgttt
      181 gtgtattcgg agccagtgta gtttttcttt ttactaacga tacgggtaat aattgattgc
      241 cttttacagc ctgaccttga gacaattgca aaagtctgtc cacagaggtt tgacctaata
      301 atctaaaatc ttgtttgata gtagtaagtg gaggtatgta gcaactagaa tcttctgtat
      361 catcataacc cacaactgaa atatcagccc caactctaag tccactttca gtaatagctc
      421 gcatagctcc taaagccatt tgatcattag caactaacat agcagtaggg acaatgcctt
      481 catttaacat ttgcattgtt tgctgaaatc ctgacatagc agaccaatct ccttctcttt
      541 ctgctattgg ttgtatttga tttcttgtta aatacttgtg ccatcctgct aatctcaatc
      601 tggcacttac tgaacttaag ggacctgcaa gtagtgcaat ttgttgatgg cctaatgcaa
      661 caaggtgttc aactcctaac cttgttccat cttcgtgaga aaaaattata gagtttattg
      721 gggtttggtc cgacacatcc aaaaacaatg ctggaacatt agtacatgct gcttcaactg
      781 caatcgcatc ttggtcatca agaggataat taattataag tccactaaca cgttgagcta
      841 ataaattatg tactgctgcc ttacaagcct ctacacctga tctctcaacc attgatacaa
      901 caacgcttgc accaagttga tcagctctcg atttaattgc ggctacaatt tgactaggcg
      961 catgtaatgc aagtgatgat gtggcaactc caattaaaag tgattgtttc cccgcaagtt
     1021 gctgagcaac gcgatttggt atataattca actctgccat agctgcttca accttctctc
     1081 ttgtttttgc agatacatga cttgcttggt tcacaactct cgaaaccgtt tggtagctaa
     1141 ccccggcata ttcagcaaca tcatacaaag taacaggttt catggatcat tcctccatga
     1201 taaaatttaa atttctgact ttggcaaatc cttttgcctg ttaaaaagaa tagcacaaaa
     1261 attaaaaaaa aagaaacgca aatagcaata gcattaaaaa taattattat tatttatttc
     1321 tttgattaaa atcaaaagcc tttaatgtat tttcttgatc tttcatgcgc ttaacggtaa
     1381 aatccaaggt attaagttca ccttccactt ttctctctct atcttctcta gaaaatcata
     1441 aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg tgagcggata
     1501 acaatttcac acagaattca ttaaagagga gaaattacat atggataaga aatactcaat
     1561 aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt
     1621 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct
     1681 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac
     1741 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc
     1801 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt
     1861 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc
     1921 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga
     1981 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca
     2041 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca
     2101 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga
     2161 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc
     2221 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg
     2281 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc
     2341 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc
     2401 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag
     2461 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga
     2521 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta
     2581 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc
     2641 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga
     2701 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa
     2761 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga
     2821 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg
     2881 aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg
     2941 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc
     3001 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt
     3061 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt
     3121 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc
     3181 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga
     3241 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt
     3301 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt
     3361 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga
     3421 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt
     3481 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat
     3541 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg
     3601 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga
     3661 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt
     3721 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt
     3781 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa
     3841 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg
     3901 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca
     3961 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga
     4021 attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct
     4081 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc
     4141 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct
     4201 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg
     4261 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat
     4321 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga
     4381 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg
     4441 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc
     4501 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga
     4561 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca
     4621 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa
     4681 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg
     4741 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt
     4801 gtccatgccc caagtcaata ttgtcaagaa aacagaagtc cagacaggcg gattctccaa
     4861 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga
     4921 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc
     4981 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac
     5041 aattctggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata
     5101 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga
     5161 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc
     5221 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
     5281 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
     5341 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
     5401 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
     5461 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
     5521 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
     5581 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
     5641 tgactaataa aagcttgatc agatctcagc ttttttttga agtgcctggc agtaagttga
     5701 aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac gaacaaaaag
     5761 atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt ttccatatga
     5821 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     5881 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     5941 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     6001 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     6061 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     6121 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     6181 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     6241 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     6301 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     6361 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     6421 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     6481 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     6541 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     6601 gcaaataatg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcatga
     6661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6841 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7381 tctttcctgc actagatccc cggcaagttc atccttaggg ttttcatatc tcttaaaaag
     7441 tcaatttctg cttgattaat ttcttgtgga ttattgtttt tgcggttttt cattttttat
     7501 ctccttgagt attgaatttt ttcttatata taaatatata gcaaaaacta tttttgccaa
     7561 cttttttaca aaaaattttt tattaaaaca cttagggctt tgctaaattc tcttttaaaa
     7621 gaacttagta aagccctatg gatttaagaa ctgattatac ttacgtagta aaaatactac
     7681 agatattaat agtatagacc atatcaaaat cataatcaat ttatatttat ctaaaattgt
     7741 agtccataca aaatacaaaa aaattaggtt tttctaaatt ctattttaaa agaacttagt
     7801 aacacctaat ctctaaatta cttacaaaat tctaaaatta gatttttagt tcttcatatt
     7861 tctttaaaag ttttttaaga aaatcttttt gattttcaaa aatttcatgc atcataaaac
     7921 tagtaaattt ggattttttt ttgtaaaaat cataactttc ttgggtttta agttgaaatc
     7981 ttaatggttt tattggattt tgcctagatt tttttaacgc cggactttct ttatctttta
     8041 taaaattata agaattttta ataccatttt ctattaaata atcttctttt ataagcccct
     8101 cttgcagtgc agttgctaat cttaaatact tatacgcttg agtttttgct acatcaaagt
     8161 cttttataaa atcaagaaaa gttttataac catcaaattt ataatattga ttatctttta
     8221 tttcttttaa aattttaata ttttccaccc tgtggaatat ttcttttttg aaattgtcat
     8281 ttaacttttc ctttagtgtc ttgtaataag aaattttact ttcattaatc attaaatttt
     8341 gttcaacata agtatctatc ttttttgtta aaactttaac aacacgtttt ttttccataa
     8401 aaattttcct ttatttttgc aacattttcc ttaattgata taattgttac tattttagat
     8461 tttttaatat ccacttaaga attaatccct cactagaata ttttttatta tagaattcat
     8521 atacatgttt ttctaaatct ataaatttat caacaattgc tttattatag ggtgtatcta
     8581 tcttttcttt tcttaataat cgtctcatat tctttaaata acaacaaaca ctgccggttt
     8641 taaacctaaa ttctatgtaa tatatttttg aaaaagtata tacattttca accccgttta
     8701 tttcatactt tacaaaaaca ttatttattg gttttctata accatagtaa atgcctaaaa
     8761 atttgtcccc gtctttcata ggatatagat aaagtttttg aatctctgat ttatctaata
     8821 atttttcaaa aaaaattata caccgctttt gtttgttact aactactcta tgcaatgcct
     8881 ttaatatttt tgtgtgatat atatttttat cacctttttt ttctttcagc aggaagatac
     8941 tttcttttgg aaatttaatt ttttgttcat ttattcttgc agccttaagt ccgattttca
     9001 tatttaatcc ttatatagcc tttctattta attcttttaa actcaataaa cttttttggt
     9061 ttttcattag ttctaataat tcgcaataat atgtgcaaaa cactttattg tattctattt
     9121 tcttttggtt attcaaatat tcttttataa ttggctttag aagttcaata tttacttctt
     9181 tttttagttg ttcaataagg atattgaaaa tgtttgtttt taaatcttga taattttgtt
     9241 gagaattttc tttttttctt tcaattgact tctctaattt acgctttata ttgtttaaat
     9301 cgctatattt atgactttca ataataaaat gaggcttaaa tttgtaattt tcgtatattt
     9361 tttggaaact tgtttctaat tgtttagaat tatattcatt tttttcaaat tctttttgag
     9421 tgttgcatag aatttccttt aatttttctt gtttttcttt catacaagat ttagtaaaat
     9481 gtatattttt attttttagt aatttaattt ctattatttt tatgatttta agtattttga
     9541 ttatattatc tttatcaata tttaaattga ataaaattgt aagaaaaatt gaaagaattt
     9601 ttttacataa aaagttacat ttattgaagt aatttcttac ttgatacttt tctatttcat
     9661 taatctttct ttcttctttt atattatttt tattatttaa acactccttt aaatctacac
     9721 tcccattttt agtaaatcta tctttaaggc cccttgaaac tctattttga aatcttaaat
     9781 cttttttttc ttgaaagtat ttatagattt tttggtaaca ttcttttttt gaaaaattaa
     9841 gttgatagta tatttcggtc cccaaattta cccctaagtg tttgtgataa ttagttgtga
     9901 ctttaaattc tttttctaat ttgtaaaggt aattttgcag tgtttttagt ttaacgggtg
     9961 tttgactatt tcttttaagg ttttcattaa agtaatagag tatgtttttt tgtgtgtatt
    10021 tcttatatgt tttatttatg tacgctagtg ttgagataag gactactaat ttatactgta
    10081 atttattgta gcaagttgtg gttttactgg ttttgtttat attagataag ttttccattt
    10141 atagctcctt gaataccgga ttttttatct ataaagtata tatatcacaa ttttgattta
    10201 aaagtaaatt ctttttttaa aaatacaaaa attttttata aattatatag accatgatat
    10261 tgtattaata ctaattgatt ttaatttaag gttgaactaa aataaacata gttttgtaga
    10321 aaataatttt tcattatttt gctacttgat attggatcgt aaaaatatta gggctttaca
    10381 aagttctttt aaaagagaat ttagtaaagc cctaagtctt ttaataaaaa attttttgta
    10441 aaaaagttgg caaaaatagt ttttgctata tatttatata taagaaaaaa ttcaatactc
    10501 aaggagataa aaaatggaaa acttaaacaa taatccacaa aaaattaatc aaataaataa
    10561 gcaaactaat tttattggtt ttgaaaattt tatagaactt gataatcaag ttaatcaaac
    10621 taaagaaaaa agtaaattaa gtaagatagg taagaaatta cccggaataa gcagtcaagt
    10681 ctagtgcggc cggaaacagc tatgacatga ttacgaattc gagctccttg ttttcaatga
    10741 taggtttttt aggtagagac ttgtaagggt ttgttcctgg aatataattt ttaggaattt
    10801 tcatatattt tactcctatt taactagcta acctataatt ctattatttt ttttgttttt
    10861 atgcaatcaa tattgatatt ttgatttttt atgattagaa tcatcatgag tgttattaag
    10921 cattctttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
    10981 gaaaaagtgg caccgagtcg gtgctttttt t
//
