LOCUS       sequence-307061-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        641..675
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        697..772
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2215..2229,2230..2235,2236..2252,2253..2259)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2235..2251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2267..2283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2302..2313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2320..2346,2347..2349,2350..4836,4837..4839,
                     4840..6426)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6596..7387
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7510..8098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7999..8018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatttatact gtaatttatt gtagcaagtt gtggttttac tggttttgtt tatattagat
       61 aagttttcca tttatagctc cttgaatacc ggatttttta tctataaagt atatatatca
      121 caattttgat ttaaaagtaa attctttttt taaaaataca aaaatttttt ataaattata
      181 tagaccatga tattgtatta atactaattg attttaattt aaggttgaac taaaataaac
      241 atagttttgt agaaaataat ttttcattat tttgctactt gatattggat cgtaaaaata
      301 ttagggcttt acaaagttct tttaaaagag aatttagtaa agccctaagt cttttaataa
      361 aaaatttttt gtaaaaaagt tggcaaaaat agtttttgct atatatttat atataagaaa
      421 aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa caataatcca caaaaaatta
      481 atcaaataaa taagcaaact aattttattg gttttgaaaa ttttatagaa cttgataatc
      541 aagttaatca aactaaagaa aaaagtaaat taagtaagat aggtaagaaa ttacccggaa
      601 taagcagtca agtctagtgc ggccggaatt ctaaagatct ttgacagcta gctcagtcct
      661 aggtataata ctagtgttaa tctacctaat ataccagttt tagagctaga aatagcaagt
      721 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg
      781 gcgcgccaaa taatgcccgt gtccctgttg aatagggaca cggtttttta acaatttttt
      841 tattcctagg ccttagtggc cggtacccgg ggatccttat tactggccgc tttctagcct
      901 ggatacttgt ctggctagtt gcattagaga gtcagctaga gccctagggc tagctgtttg
      961 tgtattcgga gccagtgtag tttttctttt tactaacgat acgggtaata attgattgcc
     1021 ttttacagcc tgaccttgag acaattgcaa aagtctgtcc acagaggttt gacctaataa
     1081 tctaaaatct tgtttgatag tagtaagtgg aggtatgtag caactagaat cttctgtatc
     1141 atcataaccc acaactgaaa tatcagcccc aactctaagt ccactttcag taatagctcg
     1201 catagctcct aaagccattt gatcattagc aactaacata gcagtaggga caatgccttc
     1261 atttaacatt tgcattgttt gctgaaatcc tgacatagca gaccaatctc cttctctttc
     1321 tgctattggt tgtatttgat ttcttgttaa atacttgtgc catcctgcta atctcaatct
     1381 ggcacttact gaacttaagg gacctgcaag tagtgcaatt tgttgatggc ctaatgcaac
     1441 aaggtgttca actcctaacc ttgttccatc ttcgtgagaa aaaattatag agtttattgg
     1501 ggtttggtcc gacacatcca aaaacaatgc tggaacatta gtacatgctg cttcaactgc
     1561 aatcgcatct tggtcatcaa gaggataatt aattataagt ccactaacac gttgagctaa
     1621 taaattatgt actgctgcct tacaagcctc tacacctgat ctctcaacca ttgatacaac
     1681 aacgcttgca ccaagttgat cagctctcga tttaattgcg gctacaattt gactaggcgc
     1741 atgtaatgca agtgatgatg tggcaactcc aattaaaagt gattgtttcc ccgcaagttg
     1801 ctgagcaacg cgatttggta tataattcaa ctctgccata gctgcttcaa ccttctctct
     1861 tgtttttgca gatacatgac ttgcttggtt cacaactctc gaaaccgttt ggtagctaac
     1921 cccggcatat tcagcaacat catacaaagt aacaggtttc atggatcatt cctccatgat
     1981 aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt taaaaagaat agcacaaaaa
     2041 ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat aattattatt atttatttct
     2101 ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct ttcatgcgct taacggtaaa
     2161 atccaaggta ttaagttcac cttccacttt tctctctcta tcttctctag aaaatcataa
     2221 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
     2281 caatttcaca cagaattcat taaagaggag aaattacata tggataagaa atactcaata
     2341 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     2401 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     2461 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     2521 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     2581 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     2641 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     2701 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     2761 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     2821 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     2881 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     2941 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     3001 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     3061 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     3121 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     3181 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     3241 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     3301 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     3361 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     3421 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     3481 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     3541 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     3601 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     3661 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     3721 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     3781 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     3841 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     3901 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     3961 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     4021 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     4081 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     4141 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     4201 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     4261 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     4321 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     4381 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     4441 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     4501 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     4561 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     4621 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     4681 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     4741 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     4801 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     4861 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     4921 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     4981 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     5041 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     5101 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     5161 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     5221 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     5281 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     5341 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     5401 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     5461 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     5521 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     5581 tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc agacaggcgg attctccaag
     5641 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     5701 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     5761 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     5821 attctggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     5881 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     5941 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     6001 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     6061 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     6121 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     6181 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     6241 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     6301 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     6361 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     6421 gactaataaa agcttgatca gatctcagct tttttttgaa gtgcctggca gtaagttgaa
     6481 aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg aacaaaaaga
     6541 tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt tccatatgag
     6601 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     6661 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     6721 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     6781 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     6841 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     6901 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     6961 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     7021 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7081 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7141 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7201 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7261 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7321 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7381 caaataatga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcatgac
     7441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7621 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8161 ctttcctgca ctagatcccc ggcaagttca tccttagggt tttcatatct cttaaaaagt
     8221 caatttctgc ttgattaatt tcttgtggat tattgttttt gcggtttttc attttttatc
     8281 tccttgagta ttgaattttt tcttatatat aaatatatag caaaaactat ttttgccaac
     8341 ttttttacaa aaaatttttt attaaaacac ttagggcttt gctaaattct cttttaaaag
     8401 aacttagtaa agccctatgg atttaagaac tgattatact tacgtagtaa aaatactaca
     8461 gatattaata gtatagacca tatcaaaatc ataatcaatt tatatttatc taaaattgta
     8521 gtccatacaa aatacaaaaa aattaggttt ttctaaattc tattttaaaa gaacttagta
     8581 acacctaatc tctaaattac ttacaaaatt ctaaaattag atttttagtt cttcatattt
     8641 ctttaaaagt tttttaagaa aatctttttg attttcaaaa atttcatgca tcataaaact
     8701 agtaaatttg gatttttttt tgtaaaaatc ataactttct tgggttttaa gttgaaatct
     8761 taatggtttt attggatttt gcctagattt ttttaacgcc ggactttctt tatcttttat
     8821 aaaattataa gaatttttaa taccattttc tattaaataa tcttctttta taagcccctc
     8881 ttgcagtgca gttgctaatc ttaaatactt atacgcttga gtttttgcta catcaaagtc
     8941 ttttataaaa tcaagaaaag ttttataacc atcaaattta taatattgat tatcttttat
     9001 ttcttttaaa attttaatat tttccaccct gtggaatatt tcttttttga aattgtcatt
     9061 taacttttcc tttagtgtct tgtaataaga aattttactt tcattaatca ttaaattttg
     9121 ttcaacataa gtatctatct tttttgttaa aactttaaca acacgttttt tttccataaa
     9181 aattttcctt tatttttgca acattttcct taattgatat aattgttact attttagatt
     9241 ttttaatatc cacttaagaa ttaatccctc actagaatat tttttattat agaattcata
     9301 tacatgtttt tctaaatcta taaatttatc aacaattgct ttattatagg gtgtatctat
     9361 cttttctttt cttaataatc gtctcatatt ctttaaataa caacaaacac tgccggtttt
     9421 aaacctaaat tctatgtaat atatttttga aaaagtatat acattttcaa ccccgtttat
     9481 ttcatacttt acaaaaacat tatttattgg ttttctataa ccatagtaaa tgcctaaaaa
     9541 tttgtccccg tctttcatag gatatagata aagtttttga atctctgatt tatctaataa
     9601 tttttcaaaa aaaattatac accgcttttg tttgttacta actactctat gcaatgcctt
     9661 taatattttt gtgtgatata tatttttatc accttttttt tctttcagca ggaagatact
     9721 ttcttttgga aatttaattt tttgttcatt tattcttgca gccttaagtc cgattttcat
     9781 atttaatcct tatatagcct ttctatttaa ttcttttaaa ctcaataaac ttttttggtt
     9841 tttcattagt tctaataatt cgcaataata tgtgcaaaac actttattgt attctatttt
     9901 cttttggtta ttcaaatatt cttttataat tggctttaga agttcaatat ttacttcttt
     9961 ttttagttgt tcaataagga tattgaaaat gtttgttttt aaatcttgat aattttgttg
    10021 agaattttct ttttttcttt caattgactt ctctaattta cgctttatat tgtttaaatc
    10081 gctatattta tgactttcaa taataaaatg aggcttaaat ttgtaatttt cgtatatttt
    10141 ttggaaactt gtttctaatt gtttagaatt atattcattt ttttcaaatt ctttttgagt
    10201 gttgcataga atttccttta atttttcttg tttttctttc atacaagatt tagtaaaatg
    10261 tatattttta ttttttagta atttaatttc tattattttt atgattttaa gtattttgat
    10321 tatattatct ttatcaatat ttaaattgaa taaaattgta agaaaaattg aaagaatttt
    10381 tttacataaa aagttacatt tattgaagta atttcttact tgatactttt ctatttcatt
    10441 aatctttctt tcttctttta tattattttt attatttaaa cactccttta aatctacact
    10501 cccattttta gtaaatctat ctttaaggcc ccttgaaact ctattttgaa atcttaaatc
    10561 ttttttttct tgaaagtatt tatagatttt ttggtaacat tctttttttg aaaaattaag
    10621 ttgatagtat atttcggtcc ccaaatttac ccctaagtgt ttgtgataat tagttgtgac
    10681 tttaaattct ttttctaatt tgtaaaggta attttgcagt gtttttagtt taacgggtgt
    10741 ttgactattt cttttaaggt tttcattaaa gtaatagagt atgttttttt gtgtgtattt
    10801 cttatatgtt ttatttatgt acgctagtgt tgagataagg actact
//
