LOCUS       sequence-307063-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10AC12)_Psyn-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..457
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        479..554
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2017..2033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2049..2065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2084..2095
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2102..2128,2129..2131,2132..4618,4619..4621,
                     4622..6208)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6378..7169
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7292..7880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7781..7800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaaggttga actaaaataa acatagtttt gtagaaaata atttttcatt attttgctac
       61 ttgatattgg atcgtaaaaa tattagggct ttacaaagtt cttttaaaag agaatttagt
      121 aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa gttggcaaaa atagtttttg
      181 ctatatattt atatataaga aaaaattcaa tactcaagga gataaaaaat ggaaaactta
      241 aacaataatc cacaaaaaat taatcaaata aataagcaaa ctaattttat tggttttgaa
      301 aattttatag aacttgataa tcaagttaat caaactaaag aaaaaagtaa attaagtaag
      361 ataggtaaga aattacccgg aataagcagt caagtctagt gcggccggaa ttctaaagat
      421 ctttgacagc tagctcagtc ctaggtataa tactagtgtt aatctaccta atataccagt
      481 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      541 caccgagtcg gtgctttttt tggcgcgcca aataatgccc gtgtccctgt tgaataggga
      601 cacggttttt taacaatttt tttattccta ggccttagtg gccggtaccc ggggatcctt
      661 attactggcc gctttctagc ctggatactt gtctggctag ttgcattaga gagtcagcta
      721 gagccctagg gctagctgtt tgtgtattcg gagccagtgt agtttttctt tttactaacg
      781 atacgggtaa taattgattg ccttttacag cctgaccttg agacaattgc aaaagtctgt
      841 ccacagaggt ttgacctaat aatctaaaat cttgtttgat agtagtaagt ggaggtatgt
      901 agcaactaga atcttctgta tcatcataac ccacaactga aatatcagcc ccaactctaa
      961 gtccactttc agtaatagct cgcatagctc ctaaagccat ttgatcatta gcaactaaca
     1021 tagcagtagg gacaatgcct tcatttaaca tttgcattgt ttgctgaaat cctgacatag
     1081 cagaccaatc tccttctctt tctgctattg gttgtatttg atttcttgtt aaatacttgt
     1141 gccatcctgc taatctcaat ctggcactta ctgaacttaa gggacctgca agtagtgcaa
     1201 tttgttgatg gcctaatgca acaaggtgtt caactcctaa ccttgttcca tcttcgtgag
     1261 aaaaaattat agagtttatt ggggtttggt ccgacacatc caaaaacaat gctggaacat
     1321 tagtacatgc tgcttcaact gcaatcgcat cttggtcatc aagaggataa ttaattataa
     1381 gtccactaac acgttgagct aataaattat gtactgctgc cttacaagcc tctacacctg
     1441 atctctcaac cattgataca acaacgcttg caccaagttg atcagctctc gatttaattg
     1501 cggctacaat ttgactaggc gcatgtaatg caagtgatga tgtggcaact ccaattaaaa
     1561 gtgattgttt ccccgcaagt tgctgagcaa cgcgatttgg tatataattc aactctgcca
     1621 tagctgcttc aaccttctct cttgtttttg cagatacatg acttgcttgg ttcacaactc
     1681 tcgaaaccgt ttggtagcta accccggcat attcagcaac atcatacaaa gtaacaggtt
     1741 tcatggatca ttcctccatg ataaaattta aatttctgac tttggcaaat ccttttgcct
     1801 gttaaaaaga atagcacaaa aattaaaaaa aaagaaacgc aaatagcaat agcattaaaa
     1861 ataattatta ttatttattt ctttgattaa aatcaaaagc ctttaatgta ttttcttgat
     1921 ctttcatgcg cttaacggta aaatccaagg tattaagttc accttccact tttctctctc
     1981 tatcttctct agaaaatcat aaaaaattta tttgctttgt gagcggataa caattatact
     2041 cgattcaatt gtgagcggat aacaatttca cacagaattc attaaagagg agaaattaca
     2101 tatggataag aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt
     2161 gatcactgat gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg
     2221 ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga
     2281 agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg
     2341 ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg
     2401 acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg
     2461 aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa
     2521 aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca
     2581 tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga
     2641 tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc
     2701 tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag
     2761 acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa
     2821 tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga
     2881 agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc
     2941 gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat
     3001 tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc
     3061 aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg
     3121 acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc
     3181 aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt
     3241 agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg
     3301 caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca
     3361 tgctattttg agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat
     3421 tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag
     3481 tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga
     3541 agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa
     3601 aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt
     3661 ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct
     3721 ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac
     3781 cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat
     3841 ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat
     3901 tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt
     3961 tttaacattg accttatttg aagataggga gatgattgag gaaagactta aaacatatgc
     4021 tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg
     4081 acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt
     4141 agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga
     4201 tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt
     4261 acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac
     4321 tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt
     4381 tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg
     4441 tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc
     4501 tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag
     4561 agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc
     4621 cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc
     4681 tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa
     4741 aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt
     4801 aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca
     4861 attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa
     4921 tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc
     4981 taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa
     5041 ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa
     5101 atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa
     5161 aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc
     5221 taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg
     5281 ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt
     5341 tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt
     5401 ccagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat
     5461 tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc
     5521 ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt
     5581 taaagagtta ctagggatca caattctgga aagaagttcc tttgaaaaaa atccgattga
     5641 ctttttagaa gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa
     5701 atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt
     5761 acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag
     5821 tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga
     5881 gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt
     5941 tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa
     6001 accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc
     6061 tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa
     6121 agaagtttta gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat
     6181 tgatttgagt cagctaggag gtgactaata aaagcttgat cagatctcag cttttttttg
     6241 aagtgcctgg cagtaagttg aaaaaatttg aaataaactt ggactatgtt aataaaaaaa
     6301 tacaaattaa cgaacaaaaa gatttgaatt attatataat ttagttaaaa gcaattttaa
     6361 atgagggagg tttccatatg agggaagcgg tgatcgccga agtatcgact caactatcag
     6421 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     6481 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     6541 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     6601 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     6661 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     6721 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     6781 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     6841 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     6901 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     6961 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     7021 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     7081 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     7141 gcgagatcac caaggtagtc ggcaaataat gacgtcgccg gagcggtcga gttctggacc
     7201 gaccggctcg ggttctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7921 gctggccttt tgctcacatg ttctttcctg cactagatcc ccggcaagtt catccttagg
     7981 gttttcatat ctcttaaaaa gtcaatttct gcttgattaa tttcttgtgg attattgttt
     8041 ttgcggtttt tcatttttta tctccttgag tattgaattt tttcttatat ataaatatat
     8101 agcaaaaact atttttgcca acttttttac aaaaaatttt ttattaaaac acttagggct
     8161 ttgctaaatt ctcttttaaa agaacttagt aaagccctat ggatttaaga actgattata
     8221 cttacgtagt aaaaatacta cagatattaa tagtatagac catatcaaaa tcataatcaa
     8281 tttatattta tctaaaattg tagtccatac aaaatacaaa aaaattaggt ttttctaaat
     8341 tctattttaa aagaacttag taacacctaa tctctaaatt acttacaaaa ttctaaaatt
     8401 agatttttag ttcttcatat ttctttaaaa gttttttaag aaaatctttt tgattttcaa
     8461 aaatttcatg catcataaaa ctagtaaatt tggatttttt tttgtaaaaa tcataacttt
     8521 cttgggtttt aagttgaaat cttaatggtt ttattggatt ttgcctagat ttttttaacg
     8581 ccggactttc tttatctttt ataaaattat aagaattttt aataccattt tctattaaat
     8641 aatcttcttt tataagcccc tcttgcagtg cagttgctaa tcttaaatac ttatacgctt
     8701 gagtttttgc tacatcaaag tcttttataa aatcaagaaa agttttataa ccatcaaatt
     8761 tataatattg attatctttt atttctttta aaattttaat attttccacc ctgtggaata
     8821 tttctttttt gaaattgtca tttaactttt cctttagtgt cttgtaataa gaaattttac
     8881 tttcattaat cattaaattt tgttcaacat aagtatctat cttttttgtt aaaactttaa
     8941 caacacgttt tttttccata aaaattttcc tttatttttg caacattttc cttaattgat
     9001 ataattgtta ctattttaga ttttttaata tccacttaag aattaatccc tcactagaat
     9061 attttttatt atagaattca tatacatgtt tttctaaatc tataaattta tcaacaattg
     9121 ctttattata gggtgtatct atcttttctt ttcttaataa tcgtctcata ttctttaaat
     9181 aacaacaaac actgccggtt ttaaacctaa attctatgta atatattttt gaaaaagtat
     9241 atacattttc aaccccgttt atttcatact ttacaaaaac attatttatt ggttttctat
     9301 aaccatagta aatgcctaaa aatttgtccc cgtctttcat aggatataga taaagttttt
     9361 gaatctctga tttatctaat aatttttcaa aaaaaattat acaccgcttt tgtttgttac
     9421 taactactct atgcaatgcc tttaatattt ttgtgtgata tatattttta tcaccttttt
     9481 tttctttcag caggaagata ctttcttttg gaaatttaat tttttgttca tttattcttg
     9541 cagccttaag tccgattttc atatttaatc cttatatagc ctttctattt aattctttta
     9601 aactcaataa acttttttgg tttttcatta gttctaataa ttcgcaataa tatgtgcaaa
     9661 acactttatt gtattctatt ttcttttggt tattcaaata ttcttttata attggcttta
     9721 gaagttcaat atttacttct ttttttagtt gttcaataag gatattgaaa atgtttgttt
     9781 ttaaatcttg ataattttgt tgagaatttt ctttttttct ttcaattgac ttctctaatt
     9841 tacgctttat attgtttaaa tcgctatatt tatgactttc aataataaaa tgaggcttaa
     9901 atttgtaatt ttcgtatatt ttttggaaac ttgtttctaa ttgtttagaa ttatattcat
     9961 ttttttcaaa ttctttttga gtgttgcata gaatttcctt taatttttct tgtttttctt
    10021 tcatacaaga tttagtaaaa tgtatatttt tattttttag taatttaatt tctattattt
    10081 ttatgatttt aagtattttg attatattat ctttatcaat atttaaattg aataaaattg
    10141 taagaaaaat tgaaagaatt tttttacata aaaagttaca tttattgaag taatttctta
    10201 cttgatactt ttctatttca ttaatctttc tttcttcttt tatattattt ttattattta
    10261 aacactcctt taaatctaca ctcccatttt tagtaaatct atctttaagg ccccttgaaa
    10321 ctctattttg aaatcttaaa tctttttttt cttgaaagta tttatagatt ttttggtaac
    10381 attctttttt tgaaaaatta agttgatagt atatttcggt ccccaaattt acccctaagt
    10441 gtttgtgata attagttgtg actttaaatt ctttttctaa tttgtaaagg taattttgca
    10501 gtgtttttag tttaacgggt gtttgactat ttcttttaag gttttcatta aagtaataga
    10561 gtatgttttt ttgtgtgtat ttcttatatg ttttatttat gtacgctagt gttgagataa
    10621 ggactactaa tttatactgt aatttattgt agcaagttgt ggttttactg gttttgttta
    10681 tattagataa gttttccatt tatagctcct tgaataccgg attttttatc tataaagtat
    10741 atatatcaca attttgattt aaaagtaaat tcttttttta aaaatacaaa aattttttat
    10801 aaattatata gaccatgata ttgtattaat actaattgat tttaat
//
