LOCUS       sequence-307064-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)_Psyn-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1040..1074
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1096..1171
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2614..2628,2629..2634,2635..2651,2652..2658)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2634..2650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2666..2682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2701..2712
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2719..2745,2746..2748,2749..5235,5236..5238,
                     5239..6825)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6995..7786
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7909..8497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8398..8417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcttctt ttatattatt tttattattt aaacactcct ttaaatctac actcccattt
       61 ttagtaaatc tatctttaag gccccttgaa actctatttt gaaatcttaa atcttttttt
      121 tcttgaaagt atttatagat tttttggtaa cattcttttt ttgaaaaatt aagttgatag
      181 tatatttcgg tccccaaatt tacccctaag tgtttgtgat aattagttgt gactttaaat
      241 tctttttcta atttgtaaag gtaattttgc agtgttttta gtttaacggg tgtttgacta
      301 tttcttttaa ggttttcatt aaagtaatag agtatgtttt tttgtgtgta tttcttatat
      361 gttttattta tgtacgctag tgttgagata aggactacta atttatactg taatttattg
      421 tagcaagttg tggttttact ggttttgttt atattagata agttttccat ttatagctcc
      481 ttgaataccg gattttttat ctataaagta tatatatcac aattttgatt taaaagtaaa
      541 ttcttttttt aaaaatacaa aaatttttta taaattatat agaccatgat attgtattaa
      601 tactaattga ttttaattta aggttgaact aaaataaaca tagttttgta gaaaataatt
      661 tttcattatt ttgctacttg atattggatc gtaaaaatat tagggcttta caaagttctt
      721 ttaaaagaga atttagtaaa gccctaagtc ttttaataaa aaattttttg taaaaaagtt
      781 ggcaaaaata gtttttgcta tatatttata tataagaaaa aattcaatac tcaaggagat
      841 aaaaaatgga aaacttaaac aataatccac aaaaaattaa tcaaataaat aagcaaacta
      901 attttattgg ttttgaaaat tttatagaac ttgataatca agttaatcaa actaaagaaa
      961 aaagtaaatt aagtaagata ggtaagaaat tacccggaat aagcagtcaa gtctagtgcg
     1021 gccggaattc taaagatctt tgacagctag ctcagtccta ggtataatac tagtgttaat
     1081 ctacctaata taccagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     1141 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg cgcgccaaat aatgcccgtg
     1201 tccctgttga atagggacac ggttttttaa caattttttt attcctaggc cttagtggcc
     1261 ggtacccggg gatccttatt actggccgct ttctagcctg gatacttgtc tggctagttg
     1321 cattagagag tcagctagag ccctagggct agctgtttgt gtattcggag ccagtgtagt
     1381 ttttcttttt actaacgata cgggtaataa ttgattgcct tttacagcct gaccttgaga
     1441 caattgcaaa agtctgtcca cagaggtttg acctaataat ctaaaatctt gtttgatagt
     1501 agtaagtgga ggtatgtagc aactagaatc ttctgtatca tcataaccca caactgaaat
     1561 atcagcccca actctaagtc cactttcagt aatagctcgc atagctccta aagccatttg
     1621 atcattagca actaacatag cagtagggac aatgccttca tttaacattt gcattgtttg
     1681 ctgaaatcct gacatagcag accaatctcc ttctctttct gctattggtt gtatttgatt
     1741 tcttgttaaa tacttgtgcc atcctgctaa tctcaatctg gcacttactg aacttaaggg
     1801 acctgcaagt agtgcaattt gttgatggcc taatgcaaca aggtgttcaa ctcctaacct
     1861 tgttccatct tcgtgagaaa aaattataga gtttattggg gtttggtccg acacatccaa
     1921 aaacaatgct ggaacattag tacatgctgc ttcaactgca atcgcatctt ggtcatcaag
     1981 aggataatta attataagtc cactaacacg ttgagctaat aaattatgta ctgctgcctt
     2041 acaagcctct acacctgatc tctcaaccat tgatacaaca acgcttgcac caagttgatc
     2101 agctctcgat ttaattgcgg ctacaatttg actaggcgca tgtaatgcaa gtgatgatgt
     2161 ggcaactcca attaaaagtg attgtttccc cgcaagttgc tgagcaacgc gatttggtat
     2221 ataattcaac tctgccatag ctgcttcaac cttctctctt gtttttgcag atacatgact
     2281 tgcttggttc acaactctcg aaaccgtttg gtagctaacc ccggcatatt cagcaacatc
     2341 atacaaagta acaggtttca tggatcattc ctccatgata aaatttaaat ttctgacttt
     2401 ggcaaatcct tttgcctgtt aaaaagaata gcacaaaaat taaaaaaaaa gaaacgcaaa
     2461 tagcaatagc attaaaaata attattatta tttatttctt tgattaaaat caaaagcctt
     2521 taatgtattt tcttgatctt tcatgcgctt aacggtaaaa tccaaggtat taagttcacc
     2581 ttccactttt ctctctctat cttctctaga aaatcataaa aaatttattt gctttgtgag
     2641 cggataacaa ttataacaga ttcaattgtg agcggataac aatttcacac agaattcatt
     2701 aaagaggaga aattacatat ggataagaaa tactcaatag gcttagctat cggcacaaat
     2761 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
     2821 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
     2881 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
     2941 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
     3001 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
     3061 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
     3121 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
     3181 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta
     3241 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa
     3301 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca
     3361 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa
     3421 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca
     3481 aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat
     3541 ttagataatt tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag
     3601 aatttatcag atgctatttt actttcagat atcctaagag taaatactga aataactaag
     3661 gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt
     3721 ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa
     3781 tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa
     3841 tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat
     3901 cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt
     3961 cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa
     4021 gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta tgttggtcca
     4081 ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc
     4141 ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc
     4201 atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt
     4261 tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg
     4321 cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa
     4381 acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt
     4441 tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac
     4501 catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat
     4561 atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa
     4621 agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc
     4681 cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa
     4741 tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg
     4801 cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct
     4861 ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa
     4921 aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat
     4981 aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag
     5041 aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag
     5101 attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat
     5161 tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt
     5221 gattatgatg tcgatgccat tgttccacaa agtttcctta aagacgattc aatagacaat
     5281 aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa
     5341 gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa
     5401 cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct
     5461 ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt
     5521 ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa
     5581 gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa
     5641 gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga
     5701 actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa
     5761 gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca
     5821 aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat
     5881 ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg
     5941 gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt
     6001 gtcaagaaaa cagaagtcca gacaggcgga ttctccaagg agtcaatttt accaaaaaga
     6061 aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt
     6121 gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg
     6181 aagaagttaa aatccgttaa agagttacta gggatcacaa ttctggaaag aagttccttt
     6241 gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta
     6301 atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg
     6361 gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat
     6421 tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa
     6481 aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt
     6541 gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat
     6601 aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg
     6661 ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa
     6721 cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt
     6781 ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actaataaaa gcttgatcag
     6841 atctcagctt ttttttgaag tgcctggcag taagttgaaa aaatttgaaa taaacttgga
     6901 ctatgttaat aaaaaaatac aaattaacga acaaaaagat ttgaattatt atataattta
     6961 gttaaaagca attttaaatg agggaggttt ccatatgagg gaagcggtga tcgccgaagt
     7021 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     7081 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     7141 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     7201 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     7261 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     7321 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     7381 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     7441 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     7501 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     7561 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     7621 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     7681 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     7741 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgac gtcgccggag
     7801 cggtcgagtt ctggaccgac cggctcgggt tctcatgacc aaaatccctt aacgtgagtt
     7861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcac tagatccccg
     8581 gcaagttcat ccttagggtt ttcatatctc ttaaaaagtc aatttctgct tgattaattt
     8641 cttgtggatt attgtttttg cggtttttca ttttttatct ccttgagtat tgaatttttt
     8701 cttatatata aatatatagc aaaaactatt tttgccaact tttttacaaa aaatttttta
     8761 ttaaaacact tagggctttg ctaaattctc ttttaaaaga acttagtaaa gccctatgga
     8821 tttaagaact gattatactt acgtagtaaa aatactacag atattaatag tatagaccat
     8881 atcaaaatca taatcaattt atatttatct aaaattgtag tccatacaaa atacaaaaaa
     8941 attaggtttt tctaaattct attttaaaag aacttagtaa cacctaatct ctaaattact
     9001 tacaaaattc taaaattaga tttttagttc ttcatatttc tttaaaagtt ttttaagaaa
     9061 atctttttga ttttcaaaaa tttcatgcat cataaaacta gtaaatttgg attttttttt
     9121 gtaaaaatca taactttctt gggttttaag ttgaaatctt aatggtttta ttggattttg
     9181 cctagatttt tttaacgccg gactttcttt atcttttata aaattataag aatttttaat
     9241 accattttct attaaataat cttcttttat aagcccctct tgcagtgcag ttgctaatct
     9301 taaatactta tacgcttgag tttttgctac atcaaagtct tttataaaat caagaaaagt
     9361 tttataacca tcaaatttat aatattgatt atcttttatt tcttttaaaa ttttaatatt
     9421 ttccaccctg tggaatattt cttttttgaa attgtcattt aacttttcct ttagtgtctt
     9481 gtaataagaa attttacttt cattaatcat taaattttgt tcaacataag tatctatctt
     9541 ttttgttaaa actttaacaa cacgtttttt ttccataaaa attttccttt atttttgcaa
     9601 cattttcctt aattgatata attgttacta ttttagattt tttaatatcc acttaagaat
     9661 taatccctca ctagaatatt ttttattata gaattcatat acatgttttt ctaaatctat
     9721 aaatttatca acaattgctt tattataggg tgtatctatc ttttcttttc ttaataatcg
     9781 tctcatattc tttaaataac aacaaacact gccggtttta aacctaaatt ctatgtaata
     9841 tatttttgaa aaagtatata cattttcaac cccgtttatt tcatacttta caaaaacatt
     9901 atttattggt tttctataac catagtaaat gcctaaaaat ttgtccccgt ctttcatagg
     9961 atatagataa agtttttgaa tctctgattt atctaataat ttttcaaaaa aaattataca
    10021 ccgcttttgt ttgttactaa ctactctatg caatgccttt aatatttttg tgtgatatat
    10081 atttttatca cctttttttt ctttcagcag gaagatactt tcttttggaa atttaatttt
    10141 ttgttcattt attcttgcag ccttaagtcc gattttcata tttaatcctt atatagcctt
    10201 tctatttaat tcttttaaac tcaataaact tttttggttt ttcattagtt ctaataattc
    10261 gcaataatat gtgcaaaaca ctttattgta ttctattttc ttttggttat tcaaatattc
    10321 ttttataatt ggctttagaa gttcaatatt tacttctttt tttagttgtt caataaggat
    10381 attgaaaatg tttgttttta aatcttgata attttgttga gaattttctt tttttctttc
    10441 aattgacttc tctaatttac gctttatatt gtttaaatcg ctatatttat gactttcaat
    10501 aataaaatga ggcttaaatt tgtaattttc gtatattttt tggaaacttg tttctaattg
    10561 tttagaatta tattcatttt tttcaaattc tttttgagtg ttgcatagaa tttcctttaa
    10621 tttttcttgt ttttctttca tacaagattt agtaaaatgt atatttttat tttttagtaa
    10681 tttaatttct attattttta tgattttaag tattttgatt atattatctt tatcaatatt
    10741 taaattgaat aaaattgtaa gaaaaattga aagaattttt ttacataaaa agttacattt
    10801 attgaagtaa tttcttactt gatacttttc tatttcatta atcttt
//
