LOCUS       sequence-307065-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_Psyn-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1131..1165
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1187..1262
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2705..2719,2720..2725,2726..2742,2743..2749)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2725..2741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2757..2773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(2810..2836,2837..2839,2840..5326,5327..5329,
                     5330..6916)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7086..7877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8000..8588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8489..8508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtaagaaaa attgaaagaa tttttttaca taaaaagtta catttattga agtaatttct
       61 tacttgatac ttttctattt cattaatctt tctttcttct tttatattat ttttattatt
      121 taaacactcc tttaaatcta cactcccatt tttagtaaat ctatctttaa ggccccttga
      181 aactctattt tgaaatctta aatctttttt ttcttgaaag tatttataga ttttttggta
      241 acattctttt tttgaaaaat taagttgata gtatatttcg gtccccaaat ttacccctaa
      301 gtgtttgtga taattagttg tgactttaaa ttctttttct aatttgtaaa ggtaattttg
      361 cagtgttttt agtttaacgg gtgtttgact atttctttta aggttttcat taaagtaata
      421 gagtatgttt ttttgtgtgt atttcttata tgttttattt atgtacgcta gtgttgagat
      481 aaggactact aatttatact gtaatttatt gtagcaagtt gtggttttac tggttttgtt
      541 tatattagat aagttttcca tttatagctc cttgaatacc ggatttttta tctataaagt
      601 atatatatca caattttgat ttaaaagtaa attctttttt taaaaataca aaaatttttt
      661 ataaattata tagaccatga tattgtatta atactaattg attttaattt aaggttgaac
      721 taaaataaac atagttttgt agaaaataat ttttcattat tttgctactt gatattggat
      781 cgtaaaaata ttagggcttt acaaagttct tttaaaagag aatttagtaa agccctaagt
      841 cttttaataa aaaatttttt gtaaaaaagt tggcaaaaat agtttttgct atatatttat
      901 atataagaaa aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa caataatcca
      961 caaaaaatta atcaaataaa taagcaaact aattttattg gttttgaaaa ttttatagaa
     1021 cttgataatc aagttaatca aactaaagaa aaaagtaaat taagtaagat aggtaagaaa
     1081 ttacccggaa taagcagtca agtctagtgc ggccggaatt ctaaagatct ttgacagcta
     1141 gctcagtcct aggtataata ctagtgttaa tctacctaat ataccagttt tagagctaga
     1201 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     1261 gctttttttg gcgcgccaaa taatgcccgt gtccctgttg aatagggaca cggtttttta
     1321 acaatttttt tattcctagg ccttagtggc cggtacccgg ggatccttat tactggccgc
     1381 tttctagcct ggatacttgt ctggctagtt gcattagaga gtcagctaga gccctagggc
     1441 tagctgtttg tgtattcgga gccagtgtag tttttctttt tactaacgat acgggtaata
     1501 attgattgcc ttttacagcc tgaccttgag acaattgcaa aagtctgtcc acagaggttt
     1561 gacctaataa tctaaaatct tgtttgatag tagtaagtgg aggtatgtag caactagaat
     1621 cttctgtatc atcataaccc acaactgaaa tatcagcccc aactctaagt ccactttcag
     1681 taatagctcg catagctcct aaagccattt gatcattagc aactaacata gcagtaggga
     1741 caatgccttc atttaacatt tgcattgttt gctgaaatcc tgacatagca gaccaatctc
     1801 cttctctttc tgctattggt tgtatttgat ttcttgttaa atacttgtgc catcctgcta
     1861 atctcaatct ggcacttact gaacttaagg gacctgcaag tagtgcaatt tgttgatggc
     1921 ctaatgcaac aaggtgttca actcctaacc ttgttccatc ttcgtgagaa aaaattatag
     1981 agtttattgg ggtttggtcc gacacatcca aaaacaatgc tggaacatta gtacatgctg
     2041 cttcaactgc aatcgcatct tggtcatcaa gaggataatt aattataagt ccactaacac
     2101 gttgagctaa taaattatgt actgctgcct tacaagcctc tacacctgat ctctcaacca
     2161 ttgatacaac aacgcttgca ccaagttgat cagctctcga tttaattgcg gctacaattt
     2221 gactaggcgc atgtaatgca agtgatgatg tggcaactcc aattaaaagt gattgtttcc
     2281 ccgcaagttg ctgagcaacg cgatttggta tataattcaa ctctgccata gctgcttcaa
     2341 ccttctctct tgtttttgca gatacatgac ttgcttggtt cacaactctc gaaaccgttt
     2401 ggtagctaac cccggcatat tcagcaacat catacaaagt aacaggtttc atggatcatt
     2461 cctccatgat aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt taaaaagaat
     2521 agcacaaaaa ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat aattattatt
     2581 atttatttct ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct ttcatgcgct
     2641 taacggtaaa atccaaggta ttaagttcac cttccacttt tctctctcta tcttctctag
     2701 aaaatcataa aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt
     2761 gagcggataa caatttcaca cagaattcat taaagaagag aaattacata tggataagaa
     2821 atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
     2881 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
     2941 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
     3001 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
     3061 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
     3121 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
     3181 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
     3241 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
     3301 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
     3361 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
     3421 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
     3481 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
     3541 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
     3601 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
     3661 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
     3721 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
     3781 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
     3841 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
     3901 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
     3961 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
     4021 ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag
     4081 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
     4141 gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
     4201 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
     4261 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
     4321 tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
     4381 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
     4441 gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
     4501 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
     4561 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
     4621 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
     4681 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
     4741 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
     4801 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
     4861 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
     4921 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
     4981 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
     5041 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
     5101 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
     5161 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
     5221 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
     5281 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca
     5341 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
     5401 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
     5461 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
     5521 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
     5581 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
     5641 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
     5701 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
     5761 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
     5821 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
     5881 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
     5941 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
     6001 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
     6061 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc agacaggcgg
     6121 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
     6181 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
     6241 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
     6301 agggatcaca attctggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
     6361 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
     6421 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
     6481 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
     6541 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
     6601 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
     6661 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
     6721 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
     6781 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
     6841 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
     6901 gctaggaggt gactaataaa agcttgatca gatctcagct tttttttgaa gtgcctggca
     6961 gtaagttgaa aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg
     7021 aacaaaaaga tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt
     7081 tccatatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7141 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7201 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7261 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     7321 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     7381 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     7441 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7501 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7561 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7621 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7681 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7741 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7801 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7861 aggtagtcgg caaataatga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7921 ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8641 ctcacatgtt ctttcctgca ctagatcccc ggcaagttca tccttagggt tttcatatct
     8701 cttaaaaagt caatttctgc ttgattaatt tcttgtggat tattgttttt gcggtttttc
     8761 attttttatc tccttgagta ttgaattttt tcttatatat aaatatatag caaaaactat
     8821 ttttgccaac ttttttacaa aaaatttttt attaaaacac ttagggcttt gctaaattct
     8881 cttttaaaag aacttagtaa agccctatgg atttaagaac tgattatact tacgtagtaa
     8941 aaatactaca gatattaata gtatagacca tatcaaaatc ataatcaatt tatatttatc
     9001 taaaattgta gtccatacaa aatacaaaaa aattaggttt ttctaaattc tattttaaaa
     9061 gaacttagta acacctaatc tctaaattac ttacaaaatt ctaaaattag atttttagtt
     9121 cttcatattt ctttaaaagt tttttaagaa aatctttttg attttcaaaa atttcatgca
     9181 tcataaaact agtaaatttg gatttttttt tgtaaaaatc ataactttct tgggttttaa
     9241 gttgaaatct taatggtttt attggatttt gcctagattt ttttaacgcc ggactttctt
     9301 tatcttttat aaaattataa gaatttttaa taccattttc tattaaataa tcttctttta
     9361 taagcccctc ttgcagtgca gttgctaatc ttaaatactt atacgcttga gtttttgcta
     9421 catcaaagtc ttttataaaa tcaagaaaag ttttataacc atcaaattta taatattgat
     9481 tatcttttat ttcttttaaa attttaatat tttccaccct gtggaatatt tcttttttga
     9541 aattgtcatt taacttttcc tttagtgtct tgtaataaga aattttactt tcattaatca
     9601 ttaaattttg ttcaacataa gtatctatct tttttgttaa aactttaaca acacgttttt
     9661 tttccataaa aattttcctt tatttttgca acattttcct taattgatat aattgttact
     9721 attttagatt ttttaatatc cacttaagaa ttaatccctc actagaatat tttttattat
     9781 agaattcata tacatgtttt tctaaatcta taaatttatc aacaattgct ttattatagg
     9841 gtgtatctat cttttctttt cttaataatc gtctcatatt ctttaaataa caacaaacac
     9901 tgccggtttt aaacctaaat tctatgtaat atatttttga aaaagtatat acattttcaa
     9961 ccccgtttat ttcatacttt acaaaaacat tatttattgg ttttctataa ccatagtaaa
    10021 tgcctaaaaa tttgtccccg tctttcatag gatatagata aagtttttga atctctgatt
    10081 tatctaataa tttttcaaaa aaaattatac accgcttttg tttgttacta actactctat
    10141 gcaatgcctt taatattttt gtgtgatata tatttttatc accttttttt tctttcagca
    10201 ggaagatact ttcttttgga aatttaattt tttgttcatt tattcttgca gccttaagtc
    10261 cgattttcat atttaatcct tatatagcct ttctatttaa ttcttttaaa ctcaataaac
    10321 ttttttggtt tttcattagt tctaataatt cgcaataata tgtgcaaaac actttattgt
    10381 attctatttt cttttggtta ttcaaatatt cttttataat tggctttaga agttcaatat
    10441 ttacttcttt ttttagttgt tcaataagga tattgaaaat gtttgttttt aaatcttgat
    10501 aattttgttg agaattttct ttttttcttt caattgactt ctctaattta cgctttatat
    10561 tgtttaaatc gctatattta tgactttcaa taataaaatg aggcttaaat ttgtaatttt
    10621 cgtatatttt ttggaaactt gtttctaatt gtttagaatt atattcattt ttttcaaatt
    10681 ctttttgagt gttgcataga atttccttta atttttcttg tttttctttc atacaagatt
    10741 tagtaaaatg tatattttta ttttttagta atttaatttc tattattttt atgattttaa
    10801 gtattttgat tatattatct ttatcaatat ttaaattgaa taaaat
//
