LOCUS       sequence-30707-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty baculovirus expression/transfer vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFBOH-LIC
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..147
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(175..208)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        223..668
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 tttcgtacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
       61 ccatcgggcg cggatctcgg tccgaaaacc atgggcagca gccatcatca tcatcatcac
      121 agcagcggcc tggttccgcg tggtagtatt atgagttctc ctcctgaaag atccataact
      181 tcgtatagca tacattatac gaagttatgc ggccgcgacg tccacatata cctgccgttc
      241 actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac
      301 tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt
      361 ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga
      421 ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat
      481 cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta
      541 tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc
      601 catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga
      661 catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg
      721 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata
      781 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc
      841 aaaaaaatga aaa
//
