LOCUS       sequence-307075-        8264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 
            Exon 7 c.576C>T, p.D192D (Synonymous Mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Niersch J, Vega-Rubin-de-Celis S, Bazarna A, Mergener S, Jendrossek 
            V, Siveke JT, Pena-Llopis S
  TITLE     A BAP1 synonymous mutation results in exon skipping, loss of 
            function and worse patient prognosis.
  JOURNAL   iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173.
            eCollection 2021 Mar 19.
  PUBMED    33681728
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2021-02-12"; volume: "24(3):102173. doi"; pages: " 
            10.1016/j.isci.2021.102173. eCollection 2021 Mar 19"
COMMENT     Sequence Label: RG6-BAP1_E7-D192D
FEATURES             Location/Qualifiers
     source          1..8264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             724..744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2193..2195,2196..2870)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2373..2393)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2591..2610
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2785..2808
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             join(2879..2881,2882..3595)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2879..2881,2882..3592)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2921..2942)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3182..3201)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3529..3550
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3621..3638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3627..3851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3897..4325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3984..4003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4194..4215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4334..4354)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4339..4668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4519..4654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4581..4600
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4735..5529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4789..4808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5399..5418
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5703..5824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5740..5759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5794..5813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5873..5889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5873..5889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5886..5908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5897..5913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5921..5927,5928..5945,5946..5951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5966..5987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6104..6121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6275..6863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6355..6374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7034..7825,7826..7894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7657..7676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7895..7999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8074..8093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttccatggat tacaaggatg acgatgacaa gggggtacct
      721 gccccaaaaa aaaaacgcaa agtggaggac ccagtaccag gatctagagg taggtgatcc
      781 tcctgctgct ttggttcagg gttttgcttg aggggggggg gtggtgattt ccttgccatg
      841 ggcagactga gcagaaaagg ccattgggac catgttctga atgcctccac ctcaaccacc
      901 ggccggtagg accaaagcca ccccgtgttt tctcaggatc tcttttccca gggagatccc
      961 tcggcccaaa gagggagatg gcaatgctgg atgtgtgcac aataattcaa caggcattgg
     1021 aacttcagca tcgatgctga atgcaattaa caatgctcaa gcagaacccc cggctccatc
     1081 agcacagtgc aggaccaaac cccatgctgc agcagtgggg ctgtctgtac ggggtgggca
     1141 atgggaaccg gggtctgctg gggctcctgc tgcttcagtg ctgccatgca gccacacatc
     1201 ctgagagctg aaagggtcgg cgtcctcacc tggtgcacac cgtagctctg ccccacagct
     1261 ttaaggcacc tggctaacct ctgcgcttct tcccttccct cctccctggc tcaggatccc
     1321 ccgagccacg ccacctccct gagaagcaga atggccttag tgcagtgcgg accatggagg
     1381 cgttccactt tgtcagctat gtgcctatca caggccggct ctttgagctg gatgggctga
     1441 aggtctaccc cattgatcat gaattcaggt agttactgca cctttctttg ttccatctct
     1501 ccacctctgc tgtgaataaa tcgcgggtcg gtgtgtcctg tgcctttccc tgcttgggaa
     1561 acgctttcct ttcattcttt cacttctctg ctgctttttg cgctctcccc atcctgctgt
     1621 gccaacctgc tctcagttct gtgctttctg tcttccatcc caacacaccc ctgggttgct
     1681 gtcttctttc tcctttcttc ctctcttgct gtgggaccaa acgtctcctg caggacctgc
     1741 gggctctgac agaggactct cgtgggggta ctgctccctc cagtggaaaa atgctccagc
     1801 agtgtcatgc aggagattta tgccatacag ttttgctctc tgctgcatgg aggggagcag
     1861 cagaagtcga tctcccccac tctggggtcc ccctcgaggg gggcacagct ggggagggaa
     1921 caagggacaa aaccaggagg gggctccgag tccttggatt tattccccct catccatgcc
     1981 ttaccttcag gtaagggcct gaacagagcc ctttacttcc tgcttctttc tcccatagct
     2041 ccctctcctt cgggtctcct ggactcagtg ccacggttgt cccattctgg gggtctgtag
     2101 ggagccagca ggagctgcgg ccgtcctact gaccctgtcc ttattgcaca ggtcaggagg
     2161 atcaggagga cgaggaggaa gaggagaccg gtgtgcgctc ctccaagaac gtcatcaagg
     2221 agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg
     2281 agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca
     2341 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
     2401 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
     2461 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2521 actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc
     2581 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     2641 tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg
     2701 gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc
     2761 ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca
     2821 tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag accgcggtgt
     2881 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2941 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3001 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3061 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3121 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3181 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3241 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3301 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3361 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3421 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3481 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3541 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagggcc
     3601 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3661 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3721 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3781 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3841 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     3901 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3961 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4021 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4081 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4141 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4201 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4261 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4321 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4441 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4501 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4621 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4681 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4741 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4801 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4861 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4921 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4981 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5041 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5101 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5161 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5221 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5281 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5341 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5401 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5461 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5521 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5581 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5641 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5701 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5821 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5941 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8041 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8101 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8161 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     8221 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
