LOCUS       sequence-307080-        8309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 
            Exon 11 c.936T>G, p.G312G (Synonymous Mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Niersch J, Vega-Rubin-de-Celis S, Bazarna A, Mergener S, Jendrossek 
            V, Siveke JT, Pena-Llopis S
  TITLE     A BAP1 synonymous mutation results in exon skipping, loss of 
            function and worse patient prognosis.
  JOURNAL   iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173.
            eCollection 2021 Mar 19.
  PUBMED    33681728
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2021-02-12"; volume: "24(3):102173. doi"; pages: " 
            10.1016/j.isci.2021.102173. eCollection 2021 Mar 19"
COMMENT     Sequence Label: RG6-BAP1_E11-G312G
FEATURES             Location/Qualifiers
     source          1..8309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             724..744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2238..2240,2241..2915)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2418..2438)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2636..2655
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2830..2853
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             join(2924..2926,2927..3640)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2924..2926,2927..3637)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2966..2987)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3227..3246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3574..3595
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3666..3683)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3672..3896
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3942..4370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4029..4048)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4239..4260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4379..4399)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4384..4713
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4564..4699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4626..4645
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4780..5574
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4834..4853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5444..5463
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5748..5869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5785..5804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5839..5858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5918..5934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5918..5934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5931..5953)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5942..5958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5966..5972,5973..5990,5991..5996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6011..6032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6149..6166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6320..6908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6400..6419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7079..7870,7871..7939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7702..7721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7940..8044)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8119..8138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttccatggat tacaaggatg acgatgacaa gggggtacct
      721 gccccaaaaa aaaaacgcaa agtggaggac ccagtaccag gatctagagg taggtgatcc
      781 tcctgctgct ttggttcagg gttttgcttg aggggggggg gtggtgattt ccttgccatg
      841 ggcagactga gcagaaaagg ccattgggac catgttctga atgcctccac ctcaaccacc
      901 ggccggtagg accaaagcca ccccgtgttt tctcaggatc tcttttccca gggagatccc
      961 tcggcccaaa gagggagatg gcaatgctgg atgtgtgcac aataattcaa caggcattgg
     1021 aacttcagca tcgatgctga atgcaattaa caatgctcaa gcagaacccc cggctccatc
     1081 agcacagtgc aggaccaaac cccatgctgc agcagtgggg ctgtctgtac ggggtgggca
     1141 atgggaaccg gggtctgctg gggctcctgc tgcttcagtg ctgccatgca gccacacatc
     1201 ctgagagctg aaagggtcgg cgtcctcacc tggtgcacac cgtagctctg ccccacagct
     1261 ttaaggcacc tggctaacct ctgcgcttct tcccttccct cctccctggc tcaggatccg
     1321 atggggcaga ggaggcggct ggttcatgcg cacaagcccc atcccacagc cctcccaaca
     1381 aacccaagct agtggtgaag cctccaggca gcagcctcaa tggggttcac cccaacccca
     1441 ctcccattgt ccagcggctg ccggcctttc tagacaatca caattatgcc aagtccccca
     1501 tgcaggaatt caggtagtta ctgcaccttt ctttgttcca tctctccacc tctgctgtga
     1561 ataaatcgcg ggtcggtgtg tcctgtgcct ttccctgctt gggaaacgct ttcctttcat
     1621 tctttcactt ctctgctgct ttttgcgctc tccccatcct gctgtgccaa cctgctctca
     1681 gttctgtgct ttctgtcttc catcccaaca cacccctggg ttgctgtctt ctttctcctt
     1741 tcttcctctc ttgctgtggg accaaacgtc tcctgcagga cctgcgggct ctgacagagg
     1801 actctcgtgg gggtactgct ccctccagtg gaaaaatgct ccagcagtgt catgcaggag
     1861 atttatgcca tacagttttg ctctctgctg catggagggg agcagcagaa gtcgatctcc
     1921 cccactctgg ggtccccctc gaggggggca cagctgggga gggaacaagg gacaaaacca
     1981 ggagggggct ccgagtcctt ggatttattc cccctcatcc atgccttacc ttcaggtaag
     2041 ggcctgaaca gagcccttta cttcctgctt ctttctccca tagctccctc tccttcgggt
     2101 ctcctggact cagtgccacg gttgtcccat tctgggggtc tgtagggagc cagcaggagc
     2161 tgcggccgtc ctactgaccc tgtccttatt gcacaggtca ggaggatcag gaggacgagg
     2221 aggaagagga gaccggtgtg cgctcctcca agaacgtcat caaggagttc atgcgcttca
     2281 aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     2341 gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc ggccccctgc
     2401 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc
     2461 accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     2521 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     2581 acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg
     2641 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     2701 gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg
     2761 tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg
     2821 tggactccaa gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg
     2881 agcgcaccga gggccgccac cacctgttcc tgtagaccgc ggtgtgagca agggcgagga
     2941 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3001 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3061 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3121 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3181 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3241 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3301 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3361 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3421 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3481 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3541 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3601 cgccgggatc actctcggca tggacgagct gtacaagtaa gggcccgttt aaacccgctg
     3661 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3721 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3781 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3841 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3901 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3961 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4021 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4081 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4141 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4201 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4261 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4321 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4381 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4441 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4501 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4561 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4621 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4681 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4741 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4801 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4861 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4921 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4981 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5041 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5101 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5161 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5221 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5281 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5341 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5401 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5461 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5521 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5581 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5641 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5701 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5881 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6001 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6061 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8041 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8101 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8161 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8221 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8281 tcgcgatgta cgggccagat atacgcgtt
//
