LOCUS       sequence-307081-        2567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator for P. tricornutum; uLoop specific syntax; EF 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_EF_tPbt
FEATURES             Location/Qualifiers
     source          1..2567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1116..1907
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1998..2567,1..19)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2487..2506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
       61 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg gtggaagggc
      121 tcggagttgt ggtaatctat gtatcctggt ggtctcgagc gcgatgccat gttgacagtt
      181 agcgtcggtg ggcacaccat cagctacacc acgctgtgtt cgaaacgagg tcggatcgta
      241 ttgacgagtg atgctttgcc gaagacgcaa atccgatctt tcttctattt agcaggtagg
      301 tacaataaag agtctgctct acagagttag aaactgaggc cgcctaagaa gacagcatct
      361 gatatacaat gaggacatgc cgattgagct acctattcta agaagtcatg acatagcacg
      421 taaagcttat cccttcccag ttctttagaa tatgggagag ggaaagacat gaaaatcaat
      481 ttgtgaagcg tatgtgttca gaaaaaagac cgtaaacaac ccattcagcg caatctacca
      541 atcagctctt ttcattcttc cctttctatc cttgcagata attggtgatg aaatcgtcgg
      601 cactcacatt tatattatta acgctcatag atttcgtcat ttggaagttt gaccagcttc
      661 tgctatctaa gcggagacct gagacgagac gagacagcct gagaatggat gcgagtaatg
      721 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
      781 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
      841 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
      901 acgatgacag agcgttgctg cctgtgatca ccgctcctct agagtcgact tgcaggcatg
      961 caagcttagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     1021 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
     1081 tatctgatca agagacagga tgaggatcgt ttcgcatgag ggaagcggtg atcgccgaag
     1141 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1201 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1261 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1321 accttttgga aacttcggct tcccctggag agagtgagat tctccgcgct gtagaagtca
     1381 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1441 ttggagaatg gcagcgcaat gacattcttg ctggtatctt cgagccagcc acgatcgaca
     1501 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     1561 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     1621 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     1681 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     1741 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     1801 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1861 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataataa tcacctagaa
     1921 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcagg
//
