LOCUS       sequence-307082-        2709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter for P. tricornutum; uLoop specific syntax; AC 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_AC_pPbt
FEATURES             Location/Qualifiers
     source          1..2709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1217..1805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1297..1316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1896..2687)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcttgatcag atattgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
       61 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
      121 cttgctaagc ttgcatgcct gcaagtcgac tctagaggag cggtgatcac aggcagcaac
      181 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
      241 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
      301 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
      361 tttgtgccga gctcattact cgcatccatt ctcaggctgt ctcgtctcgt ctcaggtctc
      421 cggaggctct ttgtgcacgt caaacgagga atttcaacga cctgcaaaca acgacagcca
      481 actcgaaaac ggcgaatgaa acaacatcaa tgtagaagaa tgaacgaaac aagcatcgca
      541 atacattttc ggtggctctt gtcttgaaat acatactcat aacctatttc gattatttta
      601 aaatgtgtca gctttaaatt aagccggcaa ttcaaatcag aaaaagcaat ttcttgtacc
      661 ttgacacagc gacaatcaca gttcactttt acagttagtc acagtcgata cgaatggtgt
      721 gaatcttcaa ttacctgact attaacagca aatagctttc aaagttaaca tgtcgggagc
      781 tcagaaccta tggcgggaac aacagcttcg atggccttcc gttcgcgtat cacagttccc
      841 agcagcgctg ctactattcg ttgttcccca ccaaaaaagc tctaacctaa gaaaggaagt
      901 aggaacagcc tcgtaggatc cctactgcgg gtctgtcact tcccccgttg ttgccagcgg
      961 agaacgatct tgacagtaaa acgtaaaaat tcaacacttt tgattgtgtg accaatccac
     1021 gtacctcctc tttacctgca atcagttgat tactttgttt tgactatcat caccaatgcg
     1081 agaccaccag gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg
     1141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1861 gttaagggat tttggtcatg cattctaggt gattattatt tgccgactac cttggtgatc
     1921 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1981 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     2041 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     2101 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2161 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2221 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2281 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc agcaagaatg
     2341 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     2401 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcact ctctccaggg
     2461 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2521 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     2581 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     2641 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgcg aaacgatcct
     2701 catcctgtc
//
