LOCUS       sequence-307131-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi mreB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0526-sgRNAmreB
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        462..537
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1980..1994,1995..2000,2001..2017,2018..2024)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2000..2016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2032..2048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2067..2078
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2085..2111,2112..2114,2115..4601,4602..4604,
                     4605..6191)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6361..7152
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7275..7863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7764..7783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatttat atataagaaa aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa
       61 caataatcca caaaaaatta atcaaataaa taagcaaact aattttattg gttttgaaaa
      121 ttttatagaa cttgataatc aagttaatca aactaaagaa aaaagtaaat taagtaagat
      181 aggtaagaaa ttacccggaa taagcagtca agtctagtgc ggccggaaac agctatgaca
      241 tgattacgaa ttcgagctcc ttgttttcaa tgataggttt tttaggtaga gacttgtaag
      301 ggtttgttcc tggaatataa tttttaggaa ttttcatata ttttactcct atttaactag
      361 ctaacctata attctattat tttttttgtt tttatgcaat caatattgat attttgattt
      421 tttatgatta gaatcatcat gatatctatt gcaacaacag agttttagag ctagaaatag
      481 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      541 ttttggcgcg ccaaataatg cccgtgtccc tgttgaatag ggacacggtt ttttaacaat
      601 ttttttattc ctaggcctta gtggccggta cccggggatc cttattactg gccgctttct
      661 agcctggata cttgtctggc tagttgcatt agagagtcag ctagagccct agggctagct
      721 gtttgtgtat tcggagccag tgtagttttt ctttttacta acgatacggg taataattga
      781 ttgcctttta cagcctgacc ttgagacaat tgcaaaagtc tgtccacaga ggtttgacct
      841 aataatctaa aatcttgttt gatagtagta agtggaggta tgtagcaact agaatcttct
      901 gtatcatcat aacccacaac tgaaatatca gccccaactc taagtccact ttcagtaata
      961 gctcgcatag ctcctaaagc catttgatca ttagcaacta acatagcagt agggacaatg
     1021 ccttcattta acatttgcat tgtttgctga aatcctgaca tagcagacca atctccttct
     1081 ctttctgcta ttggttgtat ttgatttctt gttaaatact tgtgccatcc tgctaatctc
     1141 aatctggcac ttactgaact taagggacct gcaagtagtg caatttgttg atggcctaat
     1201 gcaacaaggt gttcaactcc taaccttgtt ccatcttcgt gagaaaaaat tatagagttt
     1261 attggggttt ggtccgacac atccaaaaac aatgctggaa cattagtaca tgctgcttca
     1321 actgcaatcg catcttggtc atcaagagga taattaatta taagtccact aacacgttga
     1381 gctaataaat tatgtactgc tgccttacaa gcctctacac ctgatctctc aaccattgat
     1441 acaacaacgc ttgcaccaag ttgatcagct ctcgatttaa ttgcggctac aatttgacta
     1501 ggcgcatgta atgcaagtga tgatgtggca actccaatta aaagtgattg tttccccgca
     1561 agttgctgag caacgcgatt tggtatataa ttcaactctg ccatagctgc ttcaaccttc
     1621 tctcttgttt ttgcagatac atgacttgct tggttcacaa ctctcgaaac cgtttggtag
     1681 ctaaccccgg catattcagc aacatcatac aaagtaacag gtttcatgga tcattcctcc
     1741 atgataaaat ttaaatttct gactttggca aatccttttg cctgttaaaa agaatagcac
     1801 aaaaattaaa aaaaaagaaa cgcaaatagc aatagcatta aaaataatta ttattattta
     1861 tttctttgat taaaatcaaa agcctttaat gtattttctt gatctttcat gcgcttaacg
     1921 gtaaaatcca aggtattaag ttcaccttcc acttttctct ctctatcttc tctagaaaat
     1981 cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca attgtgagcg
     2041 gataacaatt tcacacagaa ttcattaaag aggagaaatt acatatggat aagaaatact
     2101 caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
     2161 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
     2221 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
     2281 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
     2341 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
     2401 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
     2461 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
     2521 ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
     2581 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
     2641 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
     2701 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
     2761 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
     2821 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
     2881 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
     2941 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
     3001 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
     3061 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
     3121 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
     3181 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
     3241 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
     3301 acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac
     3361 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
     3421 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
     3481 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
     3541 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
     3601 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
     3661 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
     3721 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
     3781 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
     3841 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
     3901 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
     3961 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
     4021 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
     4081 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
     4141 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
     4201 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
     4261 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
     4321 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
     4381 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
     4441 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
     4501 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
     4561 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt
     4621 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
     4681 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
     4741 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
     4801 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
     4861 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
     4921 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
     4981 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
     5041 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
     5101 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
     5161 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
     5221 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
     5281 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
     5341 tattgtccat gccccaagtc aatattgtca agaaaacaga agtccagaca ggcggattct
     5401 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
     5461 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
     5521 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
     5581 tcacaattct ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
     5641 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
     5701 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
     5761 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
     5821 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
     5881 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
     5941 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
     6001 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
     6061 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
     6121 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
     6181 gaggtgacta ataaaagctt gatcagatct cagctttttt ttgaagtgcc tggcagtaag
     6241 ttgaaaaaat ttgaaataaa cttggactat gttaataaaa aaatacaaat taacgaacaa
     6301 aaagatttga attattatat aatttagtta aaagcaattt taaatgaggg aggtttccat
     6361 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     6421 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     6481 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     6541 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     6601 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     6661 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     6721 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     6781 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     6841 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     6901 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     6961 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     7021 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     7081 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     7141 gtcggcaaat aatgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7201 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7261 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7321 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7381 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7441 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7501 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7561 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7621 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7681 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7741 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7801 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7861 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7921 atgttctttc ctgcactaga tccccggcaa gttcatcctt agggttttca tatctcttaa
     7981 aaagtcaatt tctgcttgat taatttcttg tggattattg tttttgcggt ttttcatttt
     8041 ttatctcctt gagtattgaa ttttttctta tatataaata tatagcaaaa actatttttg
     8101 ccaacttttt tacaaaaaat tttttattaa aacacttagg gctttgctaa attctctttt
     8161 aaaagaactt agtaaagccc tatggattta agaactgatt atacttacgt agtaaaaata
     8221 ctacagatat taatagtata gaccatatca aaatcataat caatttatat ttatctaaaa
     8281 ttgtagtcca tacaaaatac aaaaaaatta ggtttttcta aattctattt taaaagaact
     8341 tagtaacacc taatctctaa attacttaca aaattctaaa attagatttt tagttcttca
     8401 tatttcttta aaagtttttt aagaaaatct ttttgatttt caaaaatttc atgcatcata
     8461 aaactagtaa atttggattt ttttttgtaa aaatcataac tttcttgggt tttaagttga
     8521 aatcttaatg gttttattgg attttgccta gattttttta acgccggact ttctttatct
     8581 tttataaaat tataagaatt tttaatacca ttttctatta aataatcttc ttttataagc
     8641 ccctcttgca gtgcagttgc taatcttaaa tacttatacg cttgagtttt tgctacatca
     8701 aagtctttta taaaatcaag aaaagtttta taaccatcaa atttataata ttgattatct
     8761 tttatttctt ttaaaatttt aatattttcc accctgtgga atatttcttt tttgaaattg
     8821 tcatttaact tttcctttag tgtcttgtaa taagaaattt tactttcatt aatcattaaa
     8881 ttttgttcaa cataagtatc tatctttttt gttaaaactt taacaacacg ttttttttcc
     8941 ataaaaattt tcctttattt ttgcaacatt ttccttaatt gatataattg ttactatttt
     9001 agatttttta atatccactt aagaattaat ccctcactag aatatttttt attatagaat
     9061 tcatatacat gtttttctaa atctataaat ttatcaacaa ttgctttatt atagggtgta
     9121 tctatctttt cttttcttaa taatcgtctc atattcttta aataacaaca aacactgccg
     9181 gttttaaacc taaattctat gtaatatatt tttgaaaaag tatatacatt ttcaaccccg
     9241 tttatttcat actttacaaa aacattattt attggttttc tataaccata gtaaatgcct
     9301 aaaaatttgt ccccgtcttt cataggatat agataaagtt tttgaatctc tgatttatct
     9361 aataattttt caaaaaaaat tatacaccgc ttttgtttgt tactaactac tctatgcaat
     9421 gcctttaata tttttgtgtg atatatattt ttatcacctt ttttttcttt cagcaggaag
     9481 atactttctt ttggaaattt aattttttgt tcatttattc ttgcagcctt aagtccgatt
     9541 ttcatattta atccttatat agcctttcta tttaattctt ttaaactcaa taaacttttt
     9601 tggtttttca ttagttctaa taattcgcaa taatatgtgc aaaacacttt attgtattct
     9661 attttctttt ggttattcaa atattctttt ataattggct ttagaagttc aatatttact
     9721 tcttttttta gttgttcaat aaggatattg aaaatgtttg tttttaaatc ttgataattt
     9781 tgttgagaat tttctttttt tctttcaatt gacttctcta atttacgctt tatattgttt
     9841 aaatcgctat atttatgact ttcaataata aaatgaggct taaatttgta attttcgtat
     9901 attttttgga aacttgtttc taattgttta gaattatatt catttttttc aaattctttt
     9961 tgagtgttgc atagaatttc ctttaatttt tcttgttttt ctttcataca agatttagta
    10021 aaatgtatat ttttattttt tagtaattta atttctatta tttttatgat tttaagtatt
    10081 ttgattatat tatctttatc aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga
    10141 atttttttac ataaaaagtt acatttattg aagtaatttc ttacttgata cttttctatt
    10201 tcattaatct ttctttcttc ttttatatta tttttattat ttaaacactc ctttaaatct
    10261 acactcccat ttttagtaaa tctatcttta aggccccttg aaactctatt ttgaaatctt
    10321 aaatcttttt tttcttgaaa gtatttatag attttttggt aacattcttt ttttgaaaaa
    10381 ttaagttgat agtatatttc ggtccccaaa tttaccccta agtgtttgtg ataattagtt
    10441 gtgactttaa attctttttc taatttgtaa aggtaatttt gcagtgtttt tagtttaacg
    10501 ggtgtttgac tatttctttt aaggttttca ttaaagtaat agagtatgtt tttttgtgtg
    10561 tatttcttat atgttttatt tatgtacgct agtgttgaga taaggactac taatttatac
    10621 tgtaatttat tgtagcaagt tgtggtttta ctggttttgt ttatattaga taagttttcc
    10681 atttatagct ccttgaatac cggatttttt atctataaag tatatatatc acaattttga
    10741 tttaaaagta aattcttttt ttaaaaatac aaaaattttt tataaattat atagaccatg
    10801 atattgtatt aatactaatt gattttaatt taaggttgaa ctaaaataaa catagttttg
    10861 tagaaaataa tttttcatta ttttgctact tgatattgga tcgtaaaaat attagggctt
    10921 tacaaagttc ttttaaaaga gaatttagta aagccctaag tcttttaata aaaaattttt
    10981 tgtaaaaaag ttggcaaaaa tagtttttgc t
//
