LOCUS       sequence-307138-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        470..504
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        526..601
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2044..2058,2059..2064,2065..2081,2082..2088)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2064..2080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2096..2112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2131..2142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2149..2175,2176..2178,2179..4665,4666..4668,
                     4669..6255)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6425..7216
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7339..7927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7828..7847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaattatat agaccatgat attgtattaa tactaattga ttttaattta aggttgaact
       61 aaaataaaca tagttttgta gaaaataatt tttcattatt ttgctacttg atattggatc
      121 gtaaaaatat tagggcttta caaagttctt ttaaaagaga atttagtaaa gccctaagtc
      181 ttttaataaa aaattttttg taaaaaagtt ggcaaaaata gtttttgcta tatatttata
      241 tataagaaaa aattcaatac tcaaggagat aaaaaatgga aaacttaaac aataatccac
      301 aaaaaattaa tcaaataaat aagcaaacta attttattgg ttttgaaaat tttatagaac
      361 ttgataatca agttaatcaa actaaagaaa aaagtaaatt aagtaagata ggtaagaaat
      421 tacccggaat aagcagtcaa gtctagtgcg gccggaattc taaagatctt tgacagctag
      481 ctcagtccta ggtataatac tagtgagtgt tattaagcat tctttgtttt agagctagaa
      541 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      601 ctttttttgg cgcgccaaat aatgcccgtg tccctgttga atagggacac ggttttttaa
      661 caattttttt attcctaggc cttagtggcc ggtacccggg gatccttatt actggccgct
      721 ttctagcctg gatacttgtc tggctagttg cattagagag tcagctagag ccctagggct
      781 agctgtttgt gtattcggag ccagtgtagt ttttcttttt actaacgata cgggtaataa
      841 ttgattgcct tttacagcct gaccttgaga caattgcaaa agtctgtcca cagaggtttg
      901 acctaataat ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc aactagaatc
      961 ttctgtatca tcataaccca caactgaaat atcagcccca actctaagtc cactttcagt
     1021 aatagctcgc atagctccta aagccatttg atcattagca actaacatag cagtagggac
     1081 aatgccttca tttaacattt gcattgtttg ctgaaatcct gacatagcag accaatctcc
     1141 ttctctttct gctattggtt gtatttgatt tcttgttaaa tacttgtgcc atcctgctaa
     1201 tctcaatctg gcacttactg aacttaaggg acctgcaagt agtgcaattt gttgatggcc
     1261 taatgcaaca aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa aaattataga
     1321 gtttattggg gtttggtccg acacatccaa aaacaatgct ggaacattag tacatgctgc
     1381 ttcaactgca atcgcatctt ggtcatcaag aggataatta attataagtc cactaacacg
     1441 ttgagctaat aaattatgta ctgctgcctt acaagcctct acacctgatc tctcaaccat
     1501 tgatacaaca acgcttgcac caagttgatc agctctcgat ttaattgcgg ctacaatttg
     1561 actaggcgca tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg attgtttccc
     1621 cgcaagttgc tgagcaacgc gatttggtat ataattcaac tctgccatag ctgcttcaac
     1681 cttctctctt gtttttgcag atacatgact tgcttggttc acaactctcg aaaccgtttg
     1741 gtagctaacc ccggcatatt cagcaacatc atacaaagta acaggtttca tggatcattc
     1801 ctccatgata aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt aaaaagaata
     1861 gcacaaaaat taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata attattatta
     1921 tttatttctt tgattaaaat caaaagcctt taatgtattt tcttgatctt tcatgcgctt
     1981 aacggtaaaa tccaaggtat taagttcacc ttccactttt ctctctctat cttctctaga
     2041 aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg
     2101 agcggataac aatttcacac agaattcatt aaagaggaga aattacatat ggataagaaa
     2161 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
     2221 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
     2281 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
     2341 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     2401 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     2461 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     2521 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     2581 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     2641 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     2701 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     2761 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     2821 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     2881 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     2941 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     3001 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     3061 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     3121 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     3181 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     3241 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     3301 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     3361 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     3421 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     3481 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     3541 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     3601 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     3661 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     3721 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     3781 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     3841 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     3901 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     3961 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     4021 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     4081 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     4141 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     4201 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     4261 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     4321 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     4381 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     4441 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     4501 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     4561 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     4621 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
     4681 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     4741 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     4801 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     4861 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     4921 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     4981 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     5041 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     5101 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     5161 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     5221 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     5281 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     5341 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     5401 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtcca gacaggcgga
     5461 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     5521 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     5581 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     5641 gggatcacaa ttctggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     5701 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     5761 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     5821 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     5881 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     5941 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     6001 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     6061 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     6121 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     6181 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     6241 ctaggaggtg actaataaaa gcttgatcag atctcagctt ttttttgaag tgcctggcag
     6301 taagttgaaa aaatttgaaa taaacttgga ctatgttaat aaaaaaatac aaattaacga
     6361 acaaaaagat ttgaattatt atataattta gttaaaagca attttaaatg agggaggttt
     6421 ccatatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     6481 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     6541 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     6601 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     6661 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     6721 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     6781 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     6841 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     6901 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     6961 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     7021 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     7081 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     7141 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     7201 ggtagtcggc aaataatgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7441 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7981 tcacatgttc tttcctgcac tagatccccg gcaagttcat ccttagggtt ttcatatctc
     8041 ttaaaaagtc aatttctgct tgattaattt cttgtggatt attgtttttg cggtttttca
     8101 ttttttatct ccttgagtat tgaatttttt cttatatata aatatatagc aaaaactatt
     8161 tttgccaact tttttacaaa aaatttttta ttaaaacact tagggctttg ctaaattctc
     8221 ttttaaaaga acttagtaaa gccctatgga tttaagaact gattatactt acgtagtaaa
     8281 aatactacag atattaatag tatagaccat atcaaaatca taatcaattt atatttatct
     8341 aaaattgtag tccatacaaa atacaaaaaa attaggtttt tctaaattct attttaaaag
     8401 aacttagtaa cacctaatct ctaaattact tacaaaattc taaaattaga tttttagttc
     8461 ttcatatttc tttaaaagtt ttttaagaaa atctttttga ttttcaaaaa tttcatgcat
     8521 cataaaacta gtaaatttgg attttttttt gtaaaaatca taactttctt gggttttaag
     8581 ttgaaatctt aatggtttta ttggattttg cctagatttt tttaacgccg gactttcttt
     8641 atcttttata aaattataag aatttttaat accattttct attaaataat cttcttttat
     8701 aagcccctct tgcagtgcag ttgctaatct taaatactta tacgcttgag tttttgctac
     8761 atcaaagtct tttataaaat caagaaaagt tttataacca tcaaatttat aatattgatt
     8821 atcttttatt tcttttaaaa ttttaatatt ttccaccctg tggaatattt cttttttgaa
     8881 attgtcattt aacttttcct ttagtgtctt gtaataagaa attttacttt cattaatcat
     8941 taaattttgt tcaacataag tatctatctt ttttgttaaa actttaacaa cacgtttttt
     9001 ttccataaaa attttccttt atttttgcaa cattttcctt aattgatata attgttacta
     9061 ttttagattt tttaatatcc acttaagaat taatccctca ctagaatatt ttttattata
     9121 gaattcatat acatgttttt ctaaatctat aaatttatca acaattgctt tattataggg
     9181 tgtatctatc ttttcttttc ttaataatcg tctcatattc tttaaataac aacaaacact
     9241 gccggtttta aacctaaatt ctatgtaata tatttttgaa aaagtatata cattttcaac
     9301 cccgtttatt tcatacttta caaaaacatt atttattggt tttctataac catagtaaat
     9361 gcctaaaaat ttgtccccgt ctttcatagg atatagataa agtttttgaa tctctgattt
     9421 atctaataat ttttcaaaaa aaattataca ccgcttttgt ttgttactaa ctactctatg
     9481 caatgccttt aatatttttg tgtgatatat atttttatca cctttttttt ctttcagcag
     9541 gaagatactt tcttttggaa atttaatttt ttgttcattt attcttgcag ccttaagtcc
     9601 gattttcata tttaatcctt atatagcctt tctatttaat tcttttaaac tcaataaact
     9661 tttttggttt ttcattagtt ctaataattc gcaataatat gtgcaaaaca ctttattgta
     9721 ttctattttc ttttggttat tcaaatattc ttttataatt ggctttagaa gttcaatatt
     9781 tacttctttt tttagttgtt caataaggat attgaaaatg tttgttttta aatcttgata
     9841 attttgttga gaattttctt tttttctttc aattgacttc tctaatttac gctttatatt
     9901 gtttaaatcg ctatatttat gactttcaat aataaaatga ggcttaaatt tgtaattttc
     9961 gtatattttt tggaaacttg tttctaattg tttagaatta tattcatttt tttcaaattc
    10021 tttttgagtg ttgcatagaa tttcctttaa tttttcttgt ttttctttca tacaagattt
    10081 agtaaaatgt atatttttat tttttagtaa tttaatttct attattttta tgattttaag
    10141 tattttgatt atattatctt tatcaatatt taaattgaat aaaattgtaa gaaaaattga
    10201 aagaattttt ttacataaaa agttacattt attgaagtaa tttcttactt gatacttttc
    10261 tatttcatta atctttcttt cttcttttat attattttta ttatttaaac actcctttaa
    10321 atctacactc ccatttttag taaatctatc tttaaggccc cttgaaactc tattttgaaa
    10381 tcttaaatct tttttttctt gaaagtattt atagattttt tggtaacatt ctttttttga
    10441 aaaattaagt tgatagtata tttcggtccc caaatttacc cctaagtgtt tgtgataatt
    10501 agttgtgact ttaaattctt tttctaattt gtaaaggtaa ttttgcagtg tttttagttt
    10561 aacgggtgtt tgactatttc ttttaaggtt ttcattaaag taatagagta tgtttttttg
    10621 tgtgtatttc ttatatgttt tatttatgta cgctagtgtt gagataagga ctactaattt
    10681 atactgtaat ttattgtagc aagttgtggt tttactggtt ttgtttatat tagataagtt
    10741 ttccatttat agctccttga ataccggatt ttttatctat aaagtatata tatcacaatt
    10801 ttgatttaaa agtaaattct ttttttaaaa atacaaaaat ttttta
//
