LOCUS       sequence-30714-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus.
  JOURNAL   Pflugers Arch. 2007 Apr . 454(1):155-63.
  PUBMED    17146677
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers 
            Arch. 2007 Apr . 454(1):155-63."
COMMENT     Sequence Label: pUNIV backbone
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          168..300
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     283..302
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        345..363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     3'UTR           402..527
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     polyA_signal    622..743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(633..652)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagcgagctg ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
       61 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
      121 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      181 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      241 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      301 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
      361 aggctagcct cgagaattca cgcgtgatct ggttaccact aaaccagcct caagaacacc
      421 cgaatggagt ctctaagcta cataatacca acttacactt tacaaaatgt tgtcccccaa
      481 aatgtagcca ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa
      541 aaaaaaaaaa aaaaaaaanc cccccccccc tgcaggcggc cgcttccctt tagtgagggt
      601 taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      661 tgcagtgaaa aaaatgcttt atttgtgaaa tttgngatgc tattgcttta tttgtaacca
      721 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      781 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc
      841 gataaggatc gatccgggct ggcgtaatag cgaa
//
