LOCUS       sequence-307142-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        22..97
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1540..1554,1555..1560,1561..1577,1578..1584)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1560..1576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1592..1608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1627..1638
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1645..1671,1672..1674,1675..4161,4162..4164,
                     4165..5751)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5921..6712
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6835..7423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7324..7343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10812..10846
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
ORIGIN
        1 gaaaatttaa atttctgact tgttttagag ctagaaatag caagttaaaa taaggctagt
       61 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttggcgcg ccaaataatg
      121 cccgtgtccc tgttgaatag ggacacggtt ttttaacaat ttttttattc ctaggcctta
      181 gtggccggta cccggggatc cttattactg gccgctttct agcctggata cttgtctggc
      241 tagttgcatt agagagtcag ctagagccct agggctagct gtttgtgtat tcggagccag
      301 tgtagttttt ctttttacta acgatacggg taataattga ttgcctttta cagcctgacc
      361 ttgagacaat tgcaaaagtc tgtccacaga ggtttgacct aataatctaa aatcttgttt
      421 gatagtagta agtggaggta tgtagcaact agaatcttct gtatcatcat aacccacaac
      481 tgaaatatca gccccaactc taagtccact ttcagtaata gctcgcatag ctcctaaagc
      541 catttgatca ttagcaacta acatagcagt agggacaatg ccttcattta acatttgcat
      601 tgtttgctga aatcctgaca tagcagacca atctccttct ctttctgcta ttggttgtat
      661 ttgatttctt gttaaatact tgtgccatcc tgctaatctc aatctggcac ttactgaact
      721 taagggacct gcaagtagtg caatttgttg atggcctaat gcaacaaggt gttcaactcc
      781 taaccttgtt ccatcttcgt gagaaaaaat tatagagttt attggggttt ggtccgacac
      841 atccaaaaac aatgctggaa cattagtaca tgctgcttca actgcaatcg catcttggtc
      901 atcaagagga taattaatta taagtccact aacacgttga gctaataaat tatgtactgc
      961 tgccttacaa gcctctacac ctgatctctc aaccattgat acaacaacgc ttgcaccaag
     1021 ttgatcagct ctcgatttaa ttgcggctac aatttgacta ggcgcatgta atgcaagtga
     1081 tgatgtggca actccaatta aaagtgattg tttccccgca agttgctgag caacgcgatt
     1141 tggtatataa ttcaactctg ccatagctgc ttcaaccttc tctcttgttt ttgcagatac
     1201 atgacttgct tggttcacaa ctctcgaaac cgtttggtag ctaaccccgg catattcagc
     1261 aacatcatac aaagtaacag gtttcatgga tcattcctcc atgataaaat ttaaatttct
     1321 gactttggca aatccttttg cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa
     1381 cgcaaatagc aatagcatta aaaataatta ttattattta tttctttgat taaaatcaaa
     1441 agcctttaat gtattttctt gatctttcat gcgcttaacg gtaaaatcca aggtattaag
     1501 ttcaccttcc acttttctct ctctatcttc tctagaaaat cataaaaaat ttatttgctt
     1561 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
     1621 ttcattaaag aggagaaatt acatatggat aagaaatact caataggctt agctatcggc
     1681 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     1741 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     1801 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     1861 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     1921 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     1981 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     2041 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     2101 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     2161 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     2221 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     2281 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     2341 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     2401 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     2461 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     2521 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     2581 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     2641 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     2701 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     2761 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     2821 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     2881 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     2941 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     3001 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     3061 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     3121 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     3181 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     3241 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     3301 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     3361 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     3421 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     3481 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     3541 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     3601 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     3661 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     3721 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     3781 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     3841 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     3901 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     3961 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     4021 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     4081 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     4141 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata
     4201 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     4261 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     4321 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     4381 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     4441 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     4501 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     4561 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     4621 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     4681 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     4741 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     4801 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     4861 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     4921 aatattgtca agaaaacaga agtccagaca ggcggattct ccaaggagtc aattttacca
     4981 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     5041 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     5101 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattct ggaaagaagt
     5161 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     5221 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     5281 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     5341 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     5401 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     5461 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     5521 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     5581 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     5641 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     5701 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacta ataaaagctt
     5761 gatcagatct cagctttttt ttgaagtgcc tggcagtaag ttgaaaaaat ttgaaataaa
     5821 cttggactat gttaataaaa aaatacaaat taacgaacaa aaagatttga attattatat
     5881 aatttagtta aaagcaattt taaatgaggg aggtttccat atgagggaag cggtgatcgc
     5941 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     6001 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     6061 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     6121 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     6181 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     6241 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     6301 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     6361 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     6421 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     6481 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     6541 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     6601 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     6661 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgacgtcg
     6721 ccggagcggt cgagttctgg accgaccggc tcgggttctc atgaccaaaa tcccttaacg
     6781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcactaga
     7501 tccccggcaa gttcatcctt agggttttca tatctcttaa aaagtcaatt tctgcttgat
     7561 taatttcttg tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa
     7621 ttttttctta tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat
     7681 tttttattaa aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc
     7741 tatggattta agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata
     7801 gaccatatca aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac
     7861 aaaaaaatta ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa
     7921 attacttaca aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt
     7981 aagaaaatct ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt
     8041 ttttttgtaa aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg
     8101 attttgccta gattttttta acgccggact ttctttatct tttataaaat tataagaatt
     8161 tttaatacca ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc
     8221 taatcttaaa tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag
     8281 aaaagtttta taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt
     8341 aatattttcc accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag
     8401 tgtcttgtaa taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc
     8461 tatctttttt gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt
     8521 ttgcaacatt ttccttaatt gatataattg ttactatttt agatttttta atatccactt
     8581 aagaattaat ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa
     8641 atctataaat ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa
     8701 taatcgtctc atattcttta aataacaaca aacactgccg gttttaaacc taaattctat
     8761 gtaatatatt tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa
     8821 aacattattt attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt
     8881 cataggatat agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat
     8941 tatacaccgc ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg
     9001 atatatattt ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt
     9061 aattttttgt tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat
     9121 agcctttcta tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa
     9181 taattcgcaa taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa
     9241 atattctttt ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat
     9301 aaggatattg aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt
     9361 tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact
     9421 ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc
     9481 taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc
     9541 ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt
     9601 tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc
     9661 aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt
     9721 acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc
     9781 ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa
     9841 tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa
     9901 gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc
     9961 ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc
    10021 taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt
    10081 aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt
    10141 tatgtacgct agtgttgaga taaggactac taatttatac tgtaatttat tgtagcaagt
    10201 tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct ccttgaatac
    10261 cggatttttt atctataaag tatatatatc acaattttga tttaaaagta aattcttttt
    10321 ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt aatactaatt
    10381 gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa tttttcatta
    10441 ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc ttttaaaaga
    10501 gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag ttggcaaaaa
    10561 tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag ataaaaaatg
    10621 gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac taattttatt
    10681 ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga aaaaagtaaa
    10741 ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtg cggccggaat
    10801 tctaaagatc tttgacagct agctcagtcc taggtataat actagt
//
