LOCUS       sequence-307144-        2135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: pLV 4xSTAR-mTFP-NLS-blast
FEATURES             Location/Qualifiers
     source          1..2135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1088..1108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1158..1710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1302..1319)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1529..1548
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1731..1740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1737..2135
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gtgaaaaggc aggtgcaaag caaatcctat cccagcttct agctaggtga aaaggcaggt
       61 gcaaagcaaa tcctatccca gcttctagct agggtgaaaa ggcaggtgca aagcaaatcc
      121 tatcccagct tctagctagg tgaaaaggca ggtgcaaagc aaatcctatc ccagcttcta
      181 gctagcggcg cgccggatcc ggaggggaca agtggaggga aagtggggcc gggcgggggg
      241 tgcctgggaa gccaggctgc gctgacgtca ctgggcgcgc aattcgggct ggagcgcttt
      301 aaaaaacgag cgtgcaagca gagaggctgc tccacaccgc tcaggccgcg gtttaaacat
      361 cgatatggcc gcctcaaaag gagaagaaac gacaatgggt gttatcaagc cagacatgaa
      421 gatcaagctt aaaatggagg gtaatgttaa tggtcacgcc ttcgtcattg agggagaggg
      481 tgaaggaaag ccatacgatg gaactaatac tattaacttg gaagtgaagg agggtgcccc
      541 acttccattc tcttatgata tccttactac agccttcgca tatggaaatc gagcattcac
      601 aaaataccca gacgacattc caaattactt caagcagtca tttccagagg gatattcttg
      661 ggagagaaca atgacattcg aggacaaggg tatagttaaa gtgaaatctg atattagtat
      721 ggaagaagat tcattcatct atgagataca cctgaagggc gagaactttc ctccgaatgg
      781 accagtgatg cagaaaaaga caacaggatg ggacgcaagt actgaacgta tgtatgtcag
      841 agatggagtt ttgaaaggtg acgtgaagca taaactgttg ttggaaggag gtggacatca
      901 tcgagtggac tttaagacaa tctacagagc taagaaagct gtgaaattgc cagactatca
      961 ctttgtggat caccgaattg aaattttgaa ccatgataaa gattacaata aagtgactgt
     1021 gtatgagtca gcagtagcta gaaactcaac tgatggtatg gacgagctct acgtaagcgc
     1081 ttccaggcca aaaaagaaaa gaaaggtcat cgattagtta attaatggcc cgggaattcc
     1141 gcccctctcc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     1201 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     1261 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     1321 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     1381 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     1441 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     1501 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     1561 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     1621 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     1681 ggttttcctt tgaaaaacac gatgataata tggatatggc cacaacggtc gccaccatgg
     1741 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     1801 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     1861 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     1921 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     1981 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     2041 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     2101 aattgctgcc ctctggttat gtgtgggagg gctaa
//
