LOCUS       sequence-307145-        8389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CckA FRET Sensor with DITE motif mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Duvall SW, Childers WS
  TITLE     Design of a Histidine Kinase FRET Sensor to Detect Complex Signal 
            Integration within Living Bacteria.
  JOURNAL   ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 
            10.1021/acssensors.0c00008. Epub 2020 Jun 16.
  PUBMED    32495620
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.0c00008"; journalName: "ACS Sens"; date: 
            "2020-06-26- 26"; volume: "5"; issue: "6"; pages: "1589-1596"
COMMENT     Sequence Label: pSWD95D293A
FEATURES             Location/Qualifiers
     source          1..8389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4026..4047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5647..6351
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIA
                     LKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAK
                     YSNLGGGMDELYK"
     primer_bind     complement(6370..6386)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6397..6414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6397..6413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6406..6428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6611..7199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6691..6710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7608..8267
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7674..7693)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 cgctaccgga ctcagatcca gatcgaaatg taccggctga actggtggaa gcacgttgag
      541 cctcgtcaag gactcatgaa gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag
      601 gtgtcaaaac accgtctgtt catttggcta gacgctagag ttcttccgga tcacaagctc
      661 caggtcgtta cactcgatga cgactgctca ttcggagttt tacactcaag gttccacgag
      721 gtgtgggctc ttgcagcggg gtcgtggcac ggctcaggca atgatccgcg ctacaccatc
      781 tccaccacct tcgagacctt ccccttcccg gaaggcctga cgcccaacat agccgccgtc
      841 gactatgagg gcgacccgcg cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg
      901 ctgcgggaag cctggctcaa tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc
      961 ggctatcccg accgcgtgct gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc
     1021 accctgacca acctctacaa ccagcgcccg gcctggctgg acatggccca ccagcgcctg
     1081 gacgccgccg tcgccgccgc ctacggctgg cccgacggcc tgaccgacga cgagatcctg
     1141 gagcgcctgt tcgccctcaa ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc
     1201 caaaaccccg acctccccgg cgaaggccgg ggtccagtta catccggaac gcttggagtg
     1261 ggcccgccta cggcctcgac caacccgcag tcggcaaggg gttcgatctg gaccccggca
     1321 ttcgccgggg aggtcggcag agaggtcaga accgcgtctt gcagccctgc ttcagatgaa
     1381 ccccgcgcag gaagccgcgg cgggccaggc cggcgtcgac ggcggcgttc atctggcggt
     1441 cggcgggggc agcgccggtg acttcgcgca ccaggccccg gaacgggatg atgctgttga
     1501 tcagggtctt gccaccggcc tcggccagct tgccggctcg gttttcggcg tactcggcgc
     1561 cgaccttgta gtcgggcccc agcgccgcgt tcagcgcctt cacctcattg gccagggcct
     1621 ggcaggactt cagacccgcg aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg
     1681 gcacctcgcg cttggacagg ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct
     1741 tcttgcccgc ctcgccccag cggccggacg gttcctcggg cttggccggc ggcttgacct
     1801 gggcgtgggc gctggaggcc agcagcacgg cggcggctcc ggccatgatc agcagcttgt
     1861 tcggcatcaa aagcgccctc ccgacaaaac acgccacggg aacggcaagc ctcgccgccg
     1921 gttcccgccc gcaagtccct cggcacgaac tctaaggcct gcgcacgatc catcgccacc
     1981 acaaagcgtg tcatcccggc cgaagcgcag cggagagccg ggacccaggg ccacaagcac
     2041 ggcgcttcgt caccccctgg gtcccggata gcgctccgcg cttccgggat gacacgggtt
     2101 taagcacttg ggtgagaagc cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc
     2161 gtcgaaggcg tcgtcgccag gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag
     2221 cgatgaccag atgaaggcca tctgctccat gatcgtcggg acgctggccg gatcgaagcc
     2281 ggggtagagg tcttccacca gccacaggcc cgtgccggga tcgaaggcga agcggcagcc
     2341 gatcaggcgg aactgggcgc gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc
     2401 gtccaggtcg tcgtggtcca gcaccaggca gcggatctgc agccagccgt ggtcgggcag
     2461 caggtagaag gcgccctcgt cctgatcctc gcccgaaacc tccagccccc ggtcgatggc
     2521 ttcgacgaca tagccggccg cgcggcaggt gtcggtgagc gcggccagca gggcggcttc
     2581 ctggtcaggg gtcaggtcgg tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt
     2641 ctagcatgga ccgcccgcgc ccgtgaggcc gaggatttcg cgctggtcag acaacctact
     2701 tgccgtcccc acatgttagc gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg
     2761 gcgcttcaga cgctcgagtt ttggggagac gaccatatgg ccgacttgca gctccaggac
     2821 aaggtttcga ccggcgcccc gcgtcggcgg tttgatccat ggctggtcgg cgcggcggtg
     2881 tttttcgtgg ctgcggcggc cctctcggcg gcgccggcgc tcaaggccgg accgaccacc
     2941 ctggccggcc tgctgctgct tctgggcgtc gcgggcgtgg ctgtgttggg ccttgtcgcc
     3001 attcgcggct cagcgctttc cggcggcgac gccgaccagg ctgaggggtt catcgaggcg
     3061 ctggccgagc cggccgccct ggccgccgcc gacggtcgcg tcctggccgc caacgggccc
     3121 tggcgcgaag tcatgggcga acagcgccgc ctgcccaagg gcgtggcggg ctccagcctg
     3181 tttgcggccc tggtccaggc gcgccagggg cagatggccg agggcatgct gagcgctgga
     3241 ggaaccgact ataccgccaa ggtctcgcgc cttgcgggcg gacggctgat gatccggctt
     3301 gcgcccatcg ttgtcgctga gccggttgtg gaagacgcgt cgtcgaacgt tacgcgtcac
     3361 cggtcggcca ccgagatgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga gggcgagggc
     3481 gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3541 ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag ccgctacccc
     3601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3661 cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt gaagttcgag
     3721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3781 atcctggggc acaagctgga gtacaacttc aacagccact acgtctatat cacggccgac
     3841 aagcagaaga actgcatcaa ggctaacttc aagatccgcc acaacgttga ggacggcagc
     3901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3961 cccgacaacc actacctgag ccatcagtcc aagctgagca aagaccccaa cgagaagcgc
     4021 gatcacatgg tcctgctgga gttcgtgacc gccgccctgg aaagcggcgg cgaagatccg
     4081 atggtgagcc cggccccggt cgccgagcgc gccgccccgc cacccagctc gctggacgcc
     4141 ttcgccggag cctcgccgtt cggcgcggcc ctgctggaag gcctggagcc gttcacctcg
     4201 cgggtgctgg agaccaatcc ggcgctgacc acgatgaccg gcgccaaggc cggtgtgctg
     4261 ttcggcgatc tgatcgacgc cgcctcgcgc gccgaggccg agacgcgcct gaacgaaggc
     4321 cgcgccggtc cctacgaggt gcggttggcg cgcgatccgt cgcgcatcgc tcacctttac
     4381 ctctatcgcg ccgaaggccg cctggtggcc tacatgatcg ccgtgtccga gcagaagcag
     4441 atcgagctgc agctgtccca ggcccagaag atgcaggcca tcggccagct ggccggcggc
     4501 gtcgcgcacg acttcaacaa cctcttgacc gccatccagc tgcgtctgga cgagttgctg
     4561 catcgccatc ccgtcggtga tccgtcgtac gagggtctca acgagatccg tcagacgggc
     4621 gtgcgcgccg ccgacctcgt gcgcaagctc ttggctttct cgcgcaagca gaccgtgcag
     4681 cgcgaggtgc tggatctggg cgagctgatc agcgagttcg aggtcttgct gcgccgcctg
     4741 ctgcgcgaag acgtcaagct gatcaccgac tatggccgcg acctgccgca ggtgcgcgcc
     4801 gacaagagcc agctcgagac ggcggtcatg aacctggccg tcaacgcccg cgacgccgtg
     4861 cgcgcggcca agggcggcgg cgtcgtgcgc atccgcaccg cgcgcctgac ccgcgacgag
     4921 gcgatccagc tgggtttccc ggccgccgac ggcgacacgg ccttcattga ggtcagtgac
     4981 gatggtccgg gcattccgcc cgacgtcatg ggcaagatct tcgacccgtt cttcaccacc
     5041 aagccggtgg gcgagggtac gggcctaggc ctagccacgg tctatggcat cgttaagcag
     5101 agcgacggct ggattcacgt ccacagccgt ccgaacgaag gcgcggcctt ccgcatcttc
     5161 ctgccggtct atgaagcgcc cgccggcgcg gtcgccgtcc aggccgtcgc cgagcccgcc
     5221 aagccgcgcg ccgctcgcga cctgtcgggc gccggccgca tcctgttcgt cgaggacgag
     5281 gacgccgtgc gcagcgtcgc cgcccgcctg ctgcgcgccc gtggctacga ggtgcttgag
     5341 gcggccgacg gcgaagaggc cctgatcatc gccgaggaaa acgccggcac gatcgacctc
     5401 ttgatcagcg acgtgatcat gcccggcatc gacggcccga ccttgctgaa gaaggcgcgt
     5461 ggctatctgg ggaccgcgcc ggtgatgttc atctccggct atgccgaggc cgagttcagc
     5521 gacctcttgg aaggcgagac gggcgtgacc ttcctcccca agccgatcga catcaagacc
     5581 ctggccgagc gcgtcaagca gcagctgcag gcggcgtcga acgttacgcg tcaccggtcg
     5641 gccacctcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
     5701 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag
     5761 ggagtgcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
     5821 attcttgcca cgtcgttcat gtatggcagc cgtaccttta tcaagtaccc ggccgacatc
     5881 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
     5941 gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg cgagctcgtc
     6001 tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
     6061 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagagga
     6121 tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa cttcgtgaca
     6181 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca tgccgttgat
     6241 caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca aagagaagtg
     6301 gcagttgcca aatacagcaa ccttggtggt ggcatggacg agctgtacaa gtaaagctgc
     6361 agcccggggg atccactagt tctagagcgg ccattcactg gccgtcgttt tacaacgtcg
     6421 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6481 cagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gggggggggg
     7321 gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc
     7381 atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca
     7441 gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt
     7501 gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa
     7561 gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgaatg gagaaaaaaa
     7621 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     7681 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     7741 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     7801 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     7861 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     7921 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     7981 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     8041 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     8101 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     8161 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     8221 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaac accccttgta
     8281 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     8341 atgtaacatc agagattttg agacacaacg tggctttccc ccccccccc
//
